Expression of ND3 in 5 Tissues of Hereditary Diabetic Mongolian Gerbils

doi:10.3969/j.issn.1674-5817.2017.01.002

Abstract

Abstract: Objective To investigate the mRNA and protein expression level of NADH dehydrogenase 3(ND3) gene in 5 different tissues of diabetic gerbils, to understand the location and target tissue of ND3 in diabetic gerbil model. Methods Six gerbils for control and diabetic groups were selected respectively.The kidney, liver, skeletal muscle, brain and heart from each group were collected. The mRNA and protein expression of ND3 were evaluated by Real time-PCR and Western blotting in both groups. Results The mRNA expression of ND3 in diabetic group was extremely significantly increased in kidney, and significantly decreased in liver and skeletal muscle compared with those of control animal. While it just showed decreasing tendency in brain and increasing tendency in heart tissue. The detection of protein expression showed that the protein level was consistent with mRNA expression in all tissues except heart. Conclusions The kidney may be the target tissue of ND3 in diabetic gerbil. In addition, ND3 also exhibits effect in other 4 tissues of diabetic gerbil.

Key words: Mongolian gerbils, Type 2 diabetes, ND3, Real-time PCR, Western blotting

CLC Number:

Q95-33

LI Yin-yin, GONG Jing-jing, WU Shao-liang, LI Xiao-hong, WANG Cun-long, HUO Xue-yun, LU Jing, LV Jian-yi, LIU Xin, GUO Meng, LI Chang-long, CHEN Zhen-wen, DU Xiao-yan. Expression of ND3 in 5 Tissues of Hereditary Diabetic Mongolian Gerbils[J]. Laboratory Animal and Comparative Medicine, 2017, 37(1): 6-10.

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