Abstract: Objective To establish an the welfare impaired mice model and investigate the effect of nutritional intervention. Methods Mice undergoing poor welfare due to repeated restraint ( simulate the procedures of laboratory routine) were used to measure the biological value when they were adapting to this procedure by testing welfare related indices (neuroendocrine parameters, immune parameters, biochemical parameters，body weight and food consumption ),and the effect of nutrient agar stick was assessed by using this model. Result Restraint mice showed higher basal serum CORT, ACTH, EPI, NE and lower basal serum [3-EP, IL-2 as well as lower body weight gain and FER compared with control group. Meanwhile，WBC, spleen index and splenocyte proliferation of restraint ones decreased significantly. Compared with model group, nutrient stick group showed lower levels of CORT, ACTH, EPI, NE, higher levels of [3-EP，IL-2, WBC, spleen index and splenocytes proliferation. All the difference was significant. Conclusion The study established successfully an animal model that display the neuroendocrine disorder，immune suppression and growth suppression due to impaired welfare, and suggested that nutritional intervention do benefit to improve the neuroendocrine disorder and immune suppression.

Key words: Animal welfare, Model, Biological value, Nutritional intervention, Mice