Exploration and Practice of Building a One-Stop Service Platform for Gene-Edited Mice in University Animal Centers: A Case Study of Shanghai Jiao Tong University

doi:10.12300/j.issn.1674-5817.2025.036

Abstract

Abstract:

Gene-edited mouse models, as a core tool in modern biomedical research, play an irreplaceable role in fields such as disease mechanism analysis, drug development, and gene function studies. However, universities often face challenges including technical complexity, dispersed resources, and low management efficiency during the preparation, breeding, and data analysis of gene-edited mice. To address these issues, establishing a one-stop service platform for gene-edited mice that integrates full-chain technical services and an intelligent management system has become an inevitable choice for university laboratory animal centers to break through current difficulties. This platform must possess core functions including gene-edited mouse construction, breeding and colony expansion, biological purification, strain preservation, and endangered strain rescue, forming a whole-process technical service system covering "target design - model construction - population expansion - genetic quality control - biological sample preservation", to promote the strategic transformation of university laboratory animal centers from providing "single technical services" to providing "whole-process solutions". Taking the Laboratory Animal Center of Shanghai Jiao Tong University as an example, through technical optimization, the technical defects such as low microoperation success rate and insufficient standardization of genotype identification were solved. Through traceability management and information sharing, problems such as chaotic management of mouse strains in research groups and waste of research funds caused by repeated constructions were solved. Through systematic integration and optimization of technical services, problems such as low efficiency, resource waste, and collaboration difficulties that may be caused by fragmented service systems were solved. This paper systematically discusses the construction path and practical experience of the gene-edited mouse service platform from three perspectives: intensive management, service capacity building, and support system construction, aiming to provide referable management models and implementation paths for the construction of similar platforms in other universities.

Key words: Gene-edited mice, One-stop service platform, University laboratory animal center, Intensive management, Whole-process technical service system

CLC Number:

Q95-33

NIE Yongqiang,WANG Zhaoxia. Exploration and Practice of Building a One-Stop Service Platform for Gene-Edited Mice in University Animal Centers: A Case Study of Shanghai Jiao Tong University[J]. Laboratory Animal and Comparative Medicine, 2025, 45(5): 642-648. DOI: 10.12300/j.issn.1674-5817.2025.036.

Figures/Tables 1

References 17

[1]	VAN DER WEYDEN L, JONKERS J, ADAMS D J. The use of CRISPR/Cas9-based gene editing strategies to explore cancer gene function in mice[J]. Curr Opin Genet Dev, 2021, 66:57-62. DOI:10.1016/j.gde.2020.12.005 .
[2]	OH J, PARK C, KIM S, et al. High levels of intracellular endotrophin in adipocytes mediate COPII vesicle supplies to autophagosome to impair autophagic flux and contribute to systemic insulin resistance in obesity[J]. Metabolism, 2023, 145:155629. DOI:10.1016/j.metabol.2023.155629 .
[3]	WAYNE BARBEE R, TURNER P V. Incorporating laboratory animal science into responsible biomedical research[J]. ILAR J, 2019, 60(1):9-16. DOI:10.1093/ilar/ilz017 .
[4]	刘苗苗, 谢忠忱, 唐倩倩, 等. 清华大学实验动物中心[J]. 实验动物与比较医学, 2024, 44(2):238-239.DOI:10.12300/j.issn.1674-5817.2024.020 .
	LIU M M, XIE Z C, TANG Q Q, et al. Tsinghua University experimental animal center[J]. Lab Anim Comp Med, 2024, 44(2):238-239. DOI:10.12300/j.issn.1674-5817.2024.020 .
[5]	梅露丹, 任艳蓉, 李卓霏, 等. 云南大学实验动物中心[J]. 实验动物与比较医学, 2024, 44(3):339-340.DOI:10.12300/j.issn.1674-5817.2024.082 .
	MEI L D, REN Y R, LI Z F, et al. Yunnan university experimental animal center[J]. Lab Anim Comp Med, 2024, 44(3):339-340. DOI:10.12300/j.issn.1674-5817.2024.082 .
[6]	张彩勤. 空军军医大学实验动物中心[J]. 实验动物与比较医学, 2023, 43(2):225-226. DOI:10.12300/i.issn.1674-5817.2023.049 .
	ZHANG C Q. Air Force Medical University laboratory animal center[J]. Lab Anim Comp Med,2023, 43(2):225-226. DOI:10.12300/i.issn.1674-5817.2023.049 .
[7]	张倩,邓青秀,蔡林. 国内普通高校实验动物从业人员职业健康风险防控概述[J]. 实验动物与比较医学, 2025, 45(2):206-213. DOI:10.12300/j.issn.1674-5817.2024.126 .
	ZHANG Q, DENG Q X, CAI L. Review on occupational health risk prevention and control for laboratory animal practitioners in Chinese general universities[J]. Lab Anim Comp Med, 2025, 45(2):206-213. DOI:10.12300/j.issn.1674-5817.2024.126 .
[8]	马倩, 戚梦婷. 上海交通大学实验动物中心[J]. 实验动物与比较医学, 2023, 43(4):457-458. DOl: 10.12300/j.issn.1674-5817.2023.084.
	MA Q, QI M T. Shanghai Jiaotong university experimental animal center[J]. Lab Anim Comp Med, 2023, 43(4):457-458. DOl: 10.12300/j.issn.1674-5817.2023.084.
[9]	EVERETTE K A, NEWBY G A, LEVINE R M, et al. Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice[J]. Nat Biomed Eng, 2023,7(5):616-628. DOI: 10.1038/s41551-023-01026-0 .
[10]	邹爽, 孟德豪, 李贺年, 等. 基于CRISPR/Cas9技术的哺乳动物细胞基因敲入的策略优化与效率提升[J]. 生命的化学, 2024, 44(8):1433-1441. DOI: 10.13488/j.smhx.20230761 .
	ZOU S, MENG D H, LI H N, et al. Strategy optimization and efficiency improvement of gene knock-in in mammalian cells based on CRISPR/Cas9 technology[J]. Chem Life, 2024, 44(8):1433-1441. DOI: 10.13488/j.smhx.20230761 .
[11]	GAJ T, GERSBACH C A, C F Ⅲ BARBAS. ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering[J]. Trends Biotechnol, 2013,31(7):397-405. DOI: 10.1016/j.tibtech.2013.04.004 .
[12]	GUAN M, BOGANI D, MARSCHALL S, et al. In vitro fertilization in mice using the MBCD-GSH protocol[J]. Curr Protoc Mouse Biol, 2014, 4(2):67-83. DOI:10.1002/9780470 942390.mo140059 .
[13]	王芊芊, 刘迪文, 洪胜辉, 等. 小鼠生物净化技术平台的设计、运行及维护[J]. 中国比较医学杂志, 2023,33(8):122-126. DOI:10.3969/j.issn.1671-7856.2023.08.016 .
	WANG Q Q, LIU D W, HONG S H, et al. Design, operation, and maintenance of the technology platform of mouse rederi vation[J]. Chin J Comp Med, 2023,33(8):122-126. DOI:10.3969/j.issn.1671-7856.2023.08.016 .
[14]	李娜, 杨秋龙, 李思璧, 等. 模型动物生物净化方法的探讨[J]. 上海交通大学学报(农业科学版), 2014, 32(5):84-88, 94. DOI: 10.3969/J.ISSN.1671-9964.2014.05.015 .
	LI N, YANG Q L, LI S B, et al. Study on biological re-derivation for model animal[J]. J Shanghai Jiao Tong Univ Agric Sci, 2014, 32(5):84-88, 94. DOI: 10.3969/J.ISSN.1671-9964.2014.05.015 .
[15]	朱莲, 孔彭成, 王美珊, 等. 剖宫产与体外授精技术在小鼠生物净化中的应用[J]. 实验动物与比较医学, 2013, 33(3):218-221. DOI:10.3969/j.issn 1674-5817.2013.03.011 .
	ZHU L, KONG P C, WANG M S, et al. Application of caesarean and in vitro fertilization for bio-cleansing in mice[J]. Lab Anim Comp Med, 2013, 33(3):218-221. DOI:10.3969/j.issn 1674-5817.2013.03.011 .
[16]	AGCA Y, AGCA C. Cryopreservation of mouse sperm for genome banking[J]. Methods Mol Biol, 2021, 2180:401-412. DOI:10.1007/978-1-0716-0783-1_17 .
[17]	LARMAN M G, GARDNER D K. Ultrarapid vitrification of mouse oocytes and embryos[J]. Methods Mol Biol, 2014, 1092:153-165. DOI:10.1007/978-1-60327-292-6_10 .