Literature Analysis of the Preparation Elements of Animal Models of Skin Photoaging and the Data of Subjects

doi:10.12300/j.issn.1674-5817.2023.026

Abstract

Abstract:

Objective To analyze the modeling elements and subjects of the animal model of skin photoaging, and to provide a reference for the preparation and improvement of the model and a basis for the scientific evaluation of the subject. Methods By searching and collecting relevant literature on the preparation of animal models of skin photoaging from 2010 to 2022 in the China National Knowledge Infrastructure, Wanfang Database, and PubMed database, the model animal species, gender, modeling method, modeling cycle, radiation source and its distance from the modeling site, cumulative radiation volume, detection indicators, and subjects (drugs or treatments) recorded in the literature were collated and summarized, and a database was established for statistical analysis. Results 257 articles that met the inclusion criteria were selected. Among them, the most common animal model was SKH-1 hairless mice, followed by SD rats and KM mice; the gender of animals was mainly female, medium-wave ultraviolet B (UVB) was often used as the radiation source, the distance between the radiation source and the modelling site was mostly 30 cm, and the modelling period was usually 40-60 days. The cumulative dose of long-wave ultraviolet A (UVA) was between 100-150 J/cm², and the cumulative dose of UVB was between 5-10 J/cm². The tests used after model establishment were skin histopathological examination, skin tissue homogenization, fibre staining, immunoblotting, etc. Subjects included Chinese herbal medicines, Chinese herbal extracts, Chinese patent medicines, Chinese herbal compound medicines, chemical drugs, biological agents and other treatments, while the animal model of skin photoaging was also used for clinical efficacy studies of external Chinese medicine, physiotherapy and positive control drugs. Conclusion In skin photoaging animal experiments, female SKH-1 hairless mice are often used, and UVB is used as the radiation source. The modeling period is usually 40-60 days, and the minimum erythema dose (MED) is incremented week by week. The cumulative UVB irradiation dose ranges from 0 to 10 J/cm², which has the advantages of high success rate, good reproducibility and high similarity with clinical disease.

Key words: Skin photoaging, Animal model, Modeling element, Subject, SKH-1 hairless mice, Ultraviolet B (UVB)

CLC Number:

Q95-33

Yasheng DENG,Jiang LIN,Chiling GAN,et al. Literature Analysis of the Preparation Elements of Animal Models of Skin Photoaging and the Data of Subjects[J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 406-414. DOI: 10.12300/j.issn.1674-5817.2023.026.

Figures/Tables 3

References 45

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动物种类 Animal species	品种品系 Breed or strain	频次（所占百分比） Frequency (percentage/%)
小鼠Mouse	SKH-1无毛小鼠	60 (23.34)
	KM小鼠	52 (20.23)
	BALB/c小鼠	23 (8.95)
	HR-1无毛小鼠	22 (8.56)
	ICR小鼠	20 (7.78)
	C57BL/6小鼠	3 (1.17)
	Swiss小鼠	3 (1.17)
	HRM-2无毛小鼠	2 (0.78)
	未知种类无毛小鼠	2 (0.78)
大鼠Rat	SD大鼠	53 (20.62)
	Wistar大鼠	8 (3.11)
	F344大鼠	2 (0.78)
	HWY/Slc无毛大鼠	1 (0.39)
豚鼠Guinea pig	未知种类	4 (1.56)
猪Pig	广西巴马小型猪	2 (0.78)

动物种类 Animal species	品种品系 Breed or strain	频次（所占百分比） Frequency (percentage/%)
小鼠Mouse	SKH-1无毛小鼠	60 (23.34)
	KM小鼠	52 (20.23)
	BALB/c小鼠	23 (8.95)
	HR-1无毛小鼠	22 (8.56)
	ICR小鼠	20 (7.78)
	C57BL/6小鼠	3 (1.17)
	Swiss小鼠	3 (1.17)
	HRM-2无毛小鼠	2 (0.78)
	未知种类无毛小鼠	2 (0.78)
大鼠Rat	SD大鼠	53 (20.62)
	Wistar大鼠	8 (3.11)
	F344大鼠	2 (0.78)
	HWY/Slc无毛大鼠	1 (0.39)
豚鼠Guinea pig	未知种类	4 (1.56)
猪Pig	广西巴马小型猪	2 (0.78)

辐射光源与造模部位距离/cm Distance between the radiation source and the moulding site/cm	频次（所占百分比/%） Frequency (percentage/%)	累计照射剂量/（J·cm²） Cumulative exposure dose/ （J·cm²）	频次（所占百分比/%） Frequency (percentage/%）
2	1 (0.92)	UVA≤50	6 (9.09)
6.5	1 (0.92)	50＜UVA≤100	13 (19.70)
10	4 (3.67)	100＜UVA≤150	20 (30.30)
15	6 (5.50)	150＜UVA≤200	13 (19.70)
18	2 (1.84)	200＜UVA≤250	0 (0.00)
20	11 (10.09)	250＜UVA≤300	5 (7.58)
22	1 (0.92)	UVA＞300	9 (13.63)
23	2 (1.83)	UVB≤5	12 (15.58)
25	3 (2.75)	5＜UVB≤10	24 (31.17)
30	36 (33.03)	10＜UVB≤15	8 (10.39)
35	10 (9.17)	15＜UVB≤20	2 (2.60)
40	21 (19.27)	20＜UVB≤25	10 (12.99)
42	2 (1.84)	25＜UVB≤30	1 (1.30)
50	6 (5.50)	UVB＞30	20 (25.97)
100	3 (2.75)

辐射光源与造模部位距离/cm Distance between the radiation source and the moulding site/cm	频次（所占百分比/%） Frequency (percentage/%)	累计照射剂量/（J·cm²） Cumulative exposure dose/ （J·cm²）	频次（所占百分比/%） Frequency (percentage/%）
2	1 (0.92)	UVA≤50	6 (9.09)
6.5	1 (0.92)	50＜UVA≤100	13 (19.70)
10	4 (3.67)	100＜UVA≤150	20 (30.30)
15	6 (5.50)	150＜UVA≤200	13 (19.70)
18	2 (1.84)	200＜UVA≤250	0 (0.00)
20	11 (10.09)	250＜UVA≤300	5 (7.58)
22	1 (0.92)	UVA＞300	9 (13.63)
23	2 (1.83)	UVB≤5	12 (15.58)
25	3 (2.75)	5＜UVB≤10	24 (31.17)
30	36 (33.03)	10＜UVB≤15	8 (10.39)
35	10 (9.17)	15＜UVB≤20	2 (2.60)
40	21 (19.27)	20＜UVB≤25	10 (12.99)
42	2 (1.84)	25＜UVB≤30	1 (1.30)
50	6 (5.50)	UVB＞30	20 (25.97)
100	3 (2.75)

分类 Classification	检测指标 Testing indicators	频次（所占百分比/%） Frequency（percentage/%）
皮肤外观特征 Skin appearance features	皮肤外观观察	80 (9.06)
	皮肤（表皮、真皮）厚度	46 (5.21)
	皱纹测定	31 (3.51)
	皮肤弹性	9 (1.02)
皮肤含水量变化 Skin moisture content change	经皮水分损失	41 (4.64)
	皮肤（角质层）水合作用测定	14 (1.58)
	皮肤含水量测定	5 (0.57)
皮肤病理检查 Dermatologic examination	皮肤组织病理检查	173 (19.59)
	纤维染色	99 (11.21)
	免疫组化	64 (7.26)
	免疫荧光	9 (1.02)
皮肤组织匀浆检测 Skin tissue homogenisation	丙二醛、羟脯氨酸、过氧化氢酶活性、透明质酸、超氧化物歧化酶活性、谷胱甘肽过氧化酶活性、皮肤组织总抗氧化能力	108 (12.23)
免疫检测 Immunity testing	免疫印迹	81 (9.17)
免疫检测 Immunity testing	酶联免疫吸附试验	26 (2.94)
PCR分析 PCR analysis	反转录PCR	30 (3.40)
PCR分析 PCR analysis	实时荧光PCR	19 (2.15)
血液检查 Blood examination	血清	19 (2.15)
血液检查 Blood examination	全血或血浆	6 (0.68)
其他 Others	β-半乳糖苷酶染色	8 (0.91)
	体重变化	9 (1.02)
	活性氧定量分析/髓过氧化物酶活性测定	6 (0.68)