Safety Evaluation on Adjuvant Heparan Sulfate in Tree Shrew

doi:10.3969/j.issn.1674-5817.2017.05.009

Abstract

Abstract: Objective To explore the feasibility of tree shrew as an animal model for safety evaluation, through the detection of adjuvant heparan sulfate (HS) of the tree shrew skin irritation and adjuvant with Hepatitis A virus attenuated live vaccine (HepA-l) combined with immune pathological analysis of main organs on tree shrews. Methods According to the dose frequency and skin integrity, the tree shrews were randomly divided into two groups, the skin irritation test performed by tree shrew own left and right contrast. According to the vaccine and adjuvant type the tree shrews randomly divided, subcutaneous immunization. Twenty weeks after immunization, major organs are harvested to make pathological slice and perform HE staining analysis. Results The skin irritation test of adjuvant HS on tree shrews were no erythema, edema and other irritating changes of single and multiple dosing group on integrity skin or damaged skin. There is no significant pathological change between HepA-l and HepA-l combined with HS group on heart, liver, spleen, lung and kidney and the brain biopsy. Conclusion The experiment proved that the tree shrew as an animal model for vaccine safety evaluation is feasible, provide the basical data for future vaccine and drug evaluation platform.

Key words: Tree shrew, Heparan sulfate(HS), Hepatitis A virus attenuated live vaccine(HepA-l), Skin irritation test, Pathological analysis, Safety evaluation

CLC Number:

Q95-33

LI Yan-han, LI Jian-fang, SUN Jing, WU Mei-ni, WANG Hai-xuan, HU Yun-zhang. Safety Evaluation on Adjuvant Heparan Sulfate in Tree Shrew[J]. Laboratory Animal and Comparative Medicine, 2017, 37(5): 390-394.

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