A Novel Mouse Model for Ulcerative Colitis-associated Carcinogenesis Induced by Azoxymethane and Dextran Sulfate Sodium

doi:10.3969/j.issn.1674-5817.2012.01.012

Abstract

Abstract: Objective To develope an ulcerative colitis-associated carcinogenesis model in mice. Methods Female BALB/c mice were randomly divided into 4 groups: the control group, the azoxymethane (AOM) group, the dextran sulfate sodium (DSS) group and the AOM + DSS group. AOM was intraperitoneally injected. DSS was fed for 1 week then with ordinary water for 2 weeks, such 3 weeks formed a cycle and 9 weeks in all. The body weight was detected weekly and the mental status, stool character, hairs glossy and mortality were daily observed and recorded. Levels of TNF-α was tested by ELISA. Inflammation and pathology were observed by HE staining. Results ①After drinking DSS,the mice showed diarrhea, bloody stool, weight loss, depressed and hairs messy. Post-oral tumor were seen anal prolapse. Non-DSS groups had no more performance. AOM + DSS group appeared one death. The body weight was significantly decreased at 4,7,8,9 week after treatment compared with the control group (P <0.05). ②The TNF-α was significantly increased in DSS groups (P<0.05). ③HE staining: DSS groups showed infiltration of inflammatory cells and (or) the formation of lymphoid follicles. The tumors were induced in all of the AOM + DSS mice (9/9), which were most located in distal colon and the pathology were showed high atypical hyperplasia and (or) carcinoma in situ. Dysplasia or cancer was not observed in the other three groups. Conclusion Ulcerative colitis-associated carcinogenesis model in mice can prepared with a single intraperitoneal injection of azoxymethane prior to three repetitive oral administrations of dextran sulfate sodium. This method have the advantages with shorter time, high formation of tumor and stability of histological type.

Key words: Ulcerative colitis, Colorectal cancer, Azoxymethane, Dextran sulfate sodium, BALB/c mice

CLC Number:

R-332
Q95-33

ZHENG Hai-ming, ZHAO Hang, ZHENG Ping. A Novel Mouse Model for Ulcerative Colitis-associated Carcinogenesis Induced by Azoxymethane and Dextran Sulfate Sodium[J]. Laboratory Animal and Comparative Medicine, 2012, 32(1): 47-50.

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