Influence of Strain, Gender and Hair Coat of Mice on Establishing Bioluminescent Imaging Pseudovirus Mouse Model

doi:10.3969/j.issn.1674-5817.2019.06.001

Abstract

Abstract: Objective To investigate the effects of strain, gender, and hair coat of mice upon establishing a bioluminescent imaging based pseudovirus mouse model. Methods C57BL/6J and BALB/c mice were infected with pseudotyped Marburg virus by intraperitoneal (ip) injection. The bioluminescent signal of mice was collected before and after removing hair to show the influence of hair upon the bioluminescent imaging signal. Meanwhile, C57BL/6J and BALB/c mice of different gender were infected with pseudotyped Ebola virus by ip and the dynamic changing process of bioluminescence over time was observed. Results The black hair coat of C57BL/6J mice blocked 82% of the bioluminescent signal while the white hair coat of BALB/c mice blocked 32%, the blocking rates are statistically different between C57BL/6J mice and BALB/c mice (P<0.001). The bioluminescent signal of male mice was stronger (P<0.05) and more homogeneous than the females. The dynamic changing process of bioluminescence showed that the peak of signals of BALB/c mice were reached on the fourth day after infection while those of C57BL/6J mice were observed on the sixth day after infection. Conclusion The strain, gender and hair of mice have significant influence upon the bioluminescent imaging based pseudovirus mouse model.

Key words: BALB/c mice, C57BL/6J mice, Gender, Hair color, Bioluminescence imaging, Pseudotyped virus

CLC Number:

Q95-33

LEI Shan, LIU Qiang, HUANG Wei-jin, WANG You-chun. Influence of Strain, Gender and Hair Coat of Mice on Establishing Bioluminescent Imaging Pseudovirus Mouse Model[J]. Laboratory Animal and Comparative Medicine, 2019, 39(6): 423-428.

References

[1] Haque A, Hober D, Blondiaux J.Addressing therapeutic options for ebola virus infection in current and future outbreaks[J]. Antimicrob Agents Chemother, 2015, 59(10): 5892-5902.
[2] Yuan S.Possible FDA-approved drugs to treat Ebola virus infection[J]. Infect Dis Poverty, 2015, 4:23.
[3] Avci P, Karimi M, Sadasivam M, et al.In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging[J]. Virulence, 2018, 9(1):28-63.
[4] Basu A, Mills DM, Bowlin TL. High-throughput screening of viral entry inhibitors using pseudotyped virus[J]. Curr Protoc Pharmacol, 2010, Chapter 13: Unit 13B 3.
[5] 王佑春, 聂建辉, 吴雪伶, 等. 假病毒检测平台的建立以及应用[J]. 中华微生物学和免疫学杂志, 2013, 33(1):40-46.
[6] Zhang L, Li Q, Liu Q, et al.A bioluminescent imaging mouse model for marburg virus based on a pseudovirus system[J]. Hum Vaccin Immunother, 2017, 13(8):1811-1817.
[7] Liu Q, Fan C, Li Q, et al.Antibody-dependent-cellular-cytotoxicity-inducing antibodies significantly affect the post-exposure treatment of Ebola virus infection[J]. Sci Rep, 2017, 30(7):45552.
[8] 吴雪伶, 何红伟, 张春涛, 等. 人乳头状瘤病毒16、45和58型假病毒小鼠感染模型的建立[J]. 中华微生物学和免疫学杂志, 2008, 28(9):791-794.
[9] 刘强, 黄维金, 聂建辉, 等. 利用活体成像技术对5型腺病毒在小鼠体内生物分布和表达的研究[J]. 中华微生物学和免疫学杂志, 2012, 32(11):925-929.
[10] 柴凡, 周耘裔, 肖庚富. 活体生物发光成像技术及其在病毒感染研究中的应用[J]. 微生物学报, 2011, 51(4):431-437.
[11] Warren TK, Warfield KL, Wells J, et al.Antiviral activity of a small-molecule inhibitor of filovirus infection[J]. Antimicrob Agents Chemother, 2010, 54(5):2152-2159.
[12] 刘燕. 炎症微环境下雌激素对大鼠BMSCs炎性因子表达及骨向分化的调控作用研究[D]. 西安: 第四军医大学, 2014.