Effect of Helicobacter hepaticus Infection on Chronic Colitis Induced by Dextran Sulfate Sodium

doi:10.12300/j.issn.1674-5817.2020.136

Abstract

Abstract: Objective To investigate the effect of Helicobacter hepaticus (H.h) infection on chronic colitis induced by dextran sulfate sodium (DSS). Methods Forty male interleukin (IL)-10^-/- mice were randomly divided into control, H.h, DSS, and H.h + DSS groups. Mice in the H.h group and H.h + DSS group were infected with H.h by gavage. Five days later, the mice in DSS group and H.h + DSS group received two 7-day cycles of a 2% DSS drink with a 5-day withdrawal time between them, while the other groups received sterilized water. At the end of the experiment, all groups of mice were sacrificed and the colon of each mouse was excised, the colon length was measured, and the colon divided into three parts for histopathological examination, cytokine mRNA transcription detection, and phosphorylation detection of signal transducer and activator of transcription 3 (STAT3). Results The pathological results showed that lymphocytes infiltration, loss of epithelium, degree of fibrosis, and crypt atrophy were more obvious in the H.h + DSS group than those in the DSS group. In addition, the transcription levels of the cytokines IL-6 and tumor necrosis factor-α (TNF-α) were upregulated, while STAT3 was activated. Conclusion H.h infection can aggravate DSS-induced chronic colitis in mice.

Key words: Helicobacter hepaticus, Chronic colitis, Dextran sulfate sodium, Mice

CLC Number:

R-332
Q95-33

WU Zhihao, SHEN Chen, YIN Jun, ZHU Liqi, ZHANG Quan. Effect of Helicobacter hepaticus Infection on Chronic Colitis Induced by Dextran Sulfate Sodium[J]. Laboratory Animal and Comparative Medicine, 2021, 41(3): 226-231.

Figures/Tables 7

References

[1] 季尚玮, 王颂, 王江滨, 等. 我国不同品系实验室小鼠肝螺杆菌感染状况调查[J]. 中华消化杂志, 2010, 30(9):597-601. DOI:10.3760/cma.j.issn.0254-1432.2010.09.006.
[2] WEHKAMP J, GÖTZ M, HERRLINGER K, et al. Inflammatory bowel disease[J]. Dtsch Arztebl Int, 2016, 113(5):72-82. DOI:10.3238/arztebl.2016.0072.
[3] KULLBERG M C, WARD J M, GORELICK P L, et al.Helicobacter hepaticus triggers colitis in specific-pathogen-free interleukin-10 (IL-10)-deficient mice through an IL-12- and gamma interferon-dependent mechanism[J]. Infect Immun, 1998, 66(11):5157-5166. DOI:10.1128/iai.66.11.5157-5166.1998.
[4] 农辉, 黄雪. 葡聚糖硫酸钠诱导结肠炎模型的研究进展[J]. 世界华人消化杂志, 2014, 22(22): 3245-3250. DOI:CNKI:SUN:XXHB.0.2014-22-013.
[5] 冯洁, 张泉, 钱淼, 等. 肝螺杆菌LAMP快速检测方法的建立及初步应用[J]. 中国动物传染病学报, 2019, 27(4):50-55.
[6] 王珏, 朱礼国, 唐幕湘, 等. 改良Masson三色染色法在胶原纤维中的应用[J]. 郧阳医学院学报, 2006(1): 44. DOI:10.3969/j.issn.1006-9674.2006.01.020.
[7] CAO S, ZHU C, FENG J, et al.Helicobacter hepaticus infection induces chronic hepatitis and fibrosis in male BALB/c mice via the activation of NF-κB, Stat3, and MAPK signaling pathways[J]. Helicobacter, 2020, 25(2):e12677. DOI:10.1111/hel.12677.
[8] NG S C, SHI H Y, HAMIDI N, et al.Worldwide incidence and prevalence of inflammatory bowel disease in the 21st century: a systematic review of population-based studies[J]. Lancet, 2017, 390(10114):2769-2778. DOI:10.1016/s0140-6736(17)32448-0.
[9] NI J, SHEN T-C D, CHEN E Z, et al. A role for bacterial urease in gut dysbiosis and Crohn's disease [J]. Sci Transl Med, 2017, 9(416):eaah6888. DOI:10.1126/scitranslmed.aah6888.
[10] C TAN C S, BERINDAN NEAGOE I, COZMA V, et al. Contribution of the IL-17/IL-23 axis to the pathogenesis of inflammatory bowel disease[J]. World J Gastroenterol, 2015, 21(19):5823-5830. DOI:10.3748/wjg.v21.i19.5823.
[11] BURKETT P R, MEYER ZU HORSTE G, KUCHROO V K. Pouring fuel on the fire: Th17 cells, the environment, and autoimmunity[J]. J Clin Invest, 2015, 125(6):2211-2219. DOI:10.1172/jci78085.
[12] GATTU S, BANG Y J, PENDSE M, et al.Epithelial retinoic acid receptor ß regulates serum amyloid A expression and vitamin A-dependent intestinal immunity[J]. Proc Natl Acad Sci USA, 2019, 116(22):10911-10916. DOI:10.1073/pnas.1812069116.
[13] BAIN C C, OLIPHANT C J, THOMSON C A, et al.Proinflammatory role of monocyte-derived CX3CR1 macrophages in Helicobacter hepaticus-induced colitis[J]. Infect Immun, 2018, 86(2): e00579-00517. DOI:10.1128/IAI.00579-17.
[14] WANG Y, YIN Y, CHEN X, et al.Induction of intestinal Th17 cells by flagellins from segmented filamentous bacteria[J]. Front Immunol, 2019, 10:2750. DOI:10.3389/fimmu.2019.02750.
[15] LUO C, ZHANG H.The role of proinflammatory pathways in the pathogenesis of colitis-associated colorectal cancer[J]. Mediators Inflamm, 2017, 2017:5126048. DOI:10.1155/2017/5126048.
[16] MATSUMOTO S, NAGAOKA M, HARA T, et al.Fucoidan derived from Cladosiphon okamuranus Tokida ameliorates murine chronic colitis through the down-regulation of interleukin-6 production on colonic epithelial cells[J]. Clin Exp Immunol, 2004, 136(3):432-439. DOI:10.1111/j.1365-2249.2004.02462.x.
[17] ALA A, DHILLON A P, HODGSON H J.Role of cell adhesion molecules in leukocyte recruitment in the liver and gut[J]. Int J Exp Pathol, 2003, 84(1):1-16. DOI:10.1046/j.1365-2613.2003.00235.x.
[18] YU H, LEE H, HERRMANN A, et al.Revisiting STAT3 signalling in cancer: new and unexpected biological functions[J]. Nat Rev Cancer, 2014, 14(11):736-746. DOI:10.1038/nrc3818.