Abstract: Objective To observe the changes of blood gas，electrolytes and energy metabolism in WHBE rabbits among different ages. Methods One hundred and thirty-three WHBE rabbits were divided into 3 groups, infant，young or senile groups respectively, the blood sample were taken from center artery in ear, and the blood gas and electrolytes indexes were detected by NOVA blood gas and electrolytes analyzers. Results (1) Effect of gender，the Glu index was lower in male infant rabbits than that of female ones(P＜0.05)，and SO₂，Hb, K+ indexes were higher in male young rabbits than those of female ones(P＜0.05)，no sexual difference was shown in the blood gas of senile group(p>0.05). (2) Effect of age, the PCO₂，BE-ECF，BE-B，SBC，HCCV，TCO₂，a/A indexes were higher in young group than those of infant group (P＜0.05，P＜0.01)，while PO₂ SO₂, O₂Ct，A, PO₂/FI indexes were lower in young group than those of infant group (P＜0.05，P＜0.01)，pH, PCO₂，BE-ECF, BE-B, SBC, HCCV, TC02, a/A, Osm indexes were higher in senile group than those of infant and young groups (P＜0.05，P＜0.01)，while PO₂, SO₂，Hct，Hb，O₂Ct，A，PO₂/FI indexes were lower in senile group than those of infant and young groups (P＜0.05，P＜0.01). Conclusion Gender has effect on Glu in infant group and on SO₂, Hb, K+ in young group. The blood gas, electrolytes and energy metabolism in WHBE rabbits were changed with increasing age, and those related with its physiological function.

Key words: WHBE rabbit, Blood gas, Electrolytes, Gender, Age