Evaluation of Proficiency Testing Results of Illuminance for animal in Laboratory Animal Facilities

doi:10.12300/j.issn.1674-5817.2025.040

Abstract

Abstract:

Objective Illuminance for animals is an important condition for maintaining circadian rhythms and safeguarding animal welfare in laboratory animal facilities. By means of open enrolment, the participating laboratories are organised to carry out on-site testing, to assess the accuracy of the testing data of the participating laboratories and to make suggestions on the testing methods. Methods The samples to be tested in this proficiency testing activity were two adjacent guinea pig rearing rooms in the laboratory animal experimental facilities. The animal lighting used COB lamps (Chip On Board Light) powered by regulated power supply as the light source, and the brightness of the light source was changed by linearly adjusting the voltage output. Split-level test sample pairs were used to set Illuminance for animals in both rooms, and the reference laboratory refers to the method required by GB 14925-2023 Laboratory Animals-Environment and Facilities for testing. Sample uniformity and stability meet the requirements of CNAS-RL02:2023 Proficiency Testing Rules, CNAS-GL002:2018 Guidelines for Statistical Treatment and Evaluation of Proficiency Testing Results, and CNAS-GL003:2018 Guidelines for Homogeneity and Stability Assessment of Proficiency Testing Samples. Each reference laboratory was assigned a 3-digit random code to conduct 2 tests for each rearing compartment respectively, and submit the test report, original records and the certificate of calibration or verification of the illuminometer on site. The assigned value for proficiency testing was the median value of each participating laboratory. The robust Z-score method was used to evaluate the test results of each laboratory, and the factors affecting the results were analysed in terms of personnel, equipment and operating environment. Results A total of 35 participating laboratories participated in the proficiency testing, completed the on-site testing and submitted the reports. 34 laboratories were rated as 'satisfactory' and 1 laboratory was rated as 'unsatisfactory'. Conclusion It was found that the number and range of illuminance measurement points of some participating laboratories were insufficient, and the test results failed to fully reflect the authenticity of the illuminance of the facilities. Illuminance for animal testing environment is dim, it is recommended to use illuminance meter with wireless probe and storage function to improve testing efficiency.

Key words: Laboratory animal, Illuminance for animal, Proficiency testing, Illuminance Meter, Individual Ventilated Cages

CLC Number:

Q95-33

LIU Wei,XU Zhongkan,HOU Fengtian,et al. Evaluation of Proficiency Testing Results of Illuminance for animal in Laboratory Animal Facilities[J]. Laboratory Animal and Comparative Medicine. DOI: 10.12300/j.issn.1674-5817.2025.040.

Figures/Tables 6

Table 1 Grubbs Test Results

项目 Item	房间1 Room 1	房间2 Room 1
实验室数量/家 Number of Laboratories	35	35
最大值/lx Maximum /lx	20.08	24.50
最小值/lx Minimum/lx	15.00	15.90
平均值/lx Average/lx	17.71	18.91
极值/lx Extreme Value/lx	15.00	24.50
标准差 Standard Deviation	1.03	1.89
G₃₅	-2.63	2.95
判定结果 Judgment Result	无离群值	无离群值

Figure 1 Distribution chart of illuminance for animal frequency resultsNote：A and B represent the distribution of illuminance data for Room 1 and Room 2， respectively. The x-axis represents the illuminance data （lx）， and the y-axis represents the frequency of the data.

Table 3 The summary statistics of the illuminance data from the 35 laboratories

统计量

Statistics

参加实验室数/个

Number of laboratories

中位值/lx

Median/lx

平均值/lx

Mean/lx

NIQR

Normalized Interquartile Range

稳健CV/%

Robust CV/%

极差/lx

Range/lx

房间1

Room 1

房间2

Room 2

Figure 2 The ZB and ZW score values tested by 35 laboratoriesNote：A represents the inter-laboratory score （ZB）， where ZB stands for the inter-laboratory score used to evaluate the consistency of measurements among the 35 different laboratories； B represents the intra-laboratory score （ZW）， where ZW stands for the intra-laboratory score used to assess the stability of measurements within the same room subjected to be tested. The numerical values are arranged from left to right in ascending order.

Figure 3 Deviation plot of illuminance for animal repeated measurement resultsNote：A and B represent the measurement results of Room 1 and Room 2 provided by the 35 different laboratories in site. The X-axis represents laboratory numbers， while the Y-axis displays the two test results （blue） and their average （yellow） obtained from participating laboratories.

Figure 4 Number of illuminance meter calibration pointsNote：The X-axis represents the quantity of test points during calibration， and the Y-axis indicates the number of calibration certificates.

References 18

1	国家市场监督管理总局, 国家标准化管理委员会. 实验动物　环境及设施: [S]. 中国标准出版社, 2023.
	Standardization Administration Of China State Administration For Market Regulation. Laboratory animal——Environment and housing facilities: [S]. Beijing: Standards Press of China, 2023.
2	孙艳艳, 林燕燕, 孙雯, 等. 光照对雌性幼鼠下丘脑—垂体—性腺轴发育的影响[J]. 中国中西医结合杂志, 2024, 44(4): 463-468. DOI: 10.7661/j.cjim.20240108.220 .
	SUN Y Y, LING Y Y, SUN W, et al. Effect of Light on Hypothalamic-Pituitary-Gonadal Axis in Young Female Golden Hamster[J]. Chinese Journal of Integrated Traditional and Western Medicin, 2024, 44(4):463-468. DOI: 10.7661/j.cjim.20240108.220 .
3	吴宪文, 陈烨, 徐国恒. 光照对大小鼠繁育性能影响的研究进展[J]. 实验动物科学, 2023, 40(4): 83-88. DOI:10.3969/j.issn.1006-6179.2023.04.016 .
	WU X W, CHEN Y, XU G H. Research Progress on Effects of Light on Reproductive Performance of Rats and Mice[J]. Laboratory Animal Science, 2023, 40(4): 83-88. DOI:10.3969/j.issn.1006-6179.2023.04.016 .
4	杜文豪, 高富敏, 李小龙, 等. 持续光照和熬夜对雌性生殖功能的影响[J]. 国际生殖健康/计划生育杂志, 2023, 42(3):245-249. DOI:10.12280/gjszjk.20230010 .
	DU W H, GAO F M, LI X L, et al. Effects of continuous light and staying up late on female reproductive function[J]. Journal of International Reproductive Health/Family Planning, 2023, 42(3):245-249. DOI:10.12280/gjszjk.20230010 .
5	李丛艳, 李钰莹, 郑洁, 等. 光照强度对四川白兔同期发情和繁殖性能的影响[J]. 畜牧与兽医, 2022, 54(8):40-44.
	LI C Y, LI Y Y, ZHENG J, et al. Effect of light intensity on estrus synchronization and reproduction performance of Sichuan white rabbit[J].Animal Husbandry & Veterinary Medicine, 2022, 54(8):40-44.
6	程伶俐. 不同光照对皮质酮注射联合慢性束缚抑郁模型小鼠的影响及其机制研究[D]. 广州: 广州医科大学, 2023. DOI: 10.27043/d.cnki.ggzyc.2023.000267 .
	CHENG L L. Effects of different light parameter on CorticosteroneChronic Restraint Stress depression model mice and its mechanism[D]. GUANGZHOU: Guangzhou Medical University, 2023. DOI:10.27043/d.cnki.ggzyc.2023.000267 .
7	宋亚玲, 边专. 生物节律在牙体硬组织发育中的作用[J]. 中华口腔医学杂志, 2023, 58(1):11-16. DOI:10.3760/cma.j.cn112144-20220930-00514 .
	SONG Y L, ZHUAN B. Effects of circadian rhythm on the development of dental hard tissues[J]. Chinese Journal of Stomatology, 2023, 58(1):11-16. DOI:10.3760/cma.j.cn112144-20220930-00514 .
8	陈莉莉. 生物节律调控口腔颌面组织生理活动及其相关疾病进程的研究进展[J]. 中华口腔医学杂志, 2022, 57(5):481-489. DOI:10.3760/cma.j.cn112144-20220228-00082 .
	CHEN L L. Advances in circadian rhythms in oral maxillofacial tissues and oral-related diseases[J]. Chinese Journal of Stomatology, 2022, 57(5):481-489. DOI:10.3760/cma.j.cn112144-20220228-00082 .
9	张凯雪, 方文卿, 陈娟, 等. 约束视距下照度诱导近视动物模型的研究[J]. 眼科, 2023, 32(4):294-298. DOI:10.13281/j.cnki.issn.1004-4469.2023.04.005 .
	ZHANG K X, FANG W Q, CHEN J, et al. Study of myopic animal model induced by illuminance under constrained visual range[J]. Ophthalmology in China, 2023, 32(4):294-298. DOI:10.13281/j.cnki.issn.1004-4469.2023.04.005 .
10	刘嘉昊, 王金兴, 胡俊杰, 等. 罗汉果甜苷V对持续光照小鼠脂肪积累的缓解作用[J]. 南方农业学报, 2022, 53(9):2624-2633. DOI:10.3969/j.issn.2095-1191.2022.09.025 .
	LIU J H, WANG J X, HU J J,et al. Mogroside V attenuated constant light exposure-induced accumulation of body fat mass in mice[J]. Journal of Southern Agriculture, 2022, 53(9):2624-2633. DOI:10.3969/j.issn.2095-1191.2022.09.025 .
11	HAWKINSHDR GOLLEDGE P..The 9 to 5 Rodent - Time for Change? Scientific and animal welfare implications of circadian and light effects on laboratory mice and rats[J]. JOURNAL OF NEUROSCIENCE METHODS, 2018, 300(20-25). DOI:10.1016/j.jneumeth.2017.05.014 .
12	National Research Council.Guide for the Care and Use of Laboratory Animals: Eighth Edition[M].Washington, DC,The National Academies Press, 2011.
13	環境省自然環境局総務課動物愛護管理室. 実験動物の飼養及び保管並びに苦痛の軽減に関する基準の解説[EB/OL].(2017-10)[2025-02-15]. .
14	On guidelines for the accommodation and care of animals used for experimental and other scientific purposes [S/OL]. (2007-06-18) 2025-02-01]. .
15	刘巍, 张心妍, 侯丰田, 等. 实验动物设施换气次数检测能力验证结果评价[J]. 实验动物与比较医学, 2025, 1(45):87-95. DOI:10.12300/j.issn.1674-5817.2024.101 .
	LIU W, ZHANG X Y, HOU F T, et al. Evaluation of the proficiency testing results of the air change rate testing capacity for laboratory animal facility[J]. Laboratory Animal and Comparative Medicine, 2025, 1(45):87-95. DOI:10.12300/j.issn.1674-5817.2024.101 .
16	住房和城乡建设部,国家市场监督管理总局. 建筑照明设计标准: [S]. 北京:中国建筑工业出版社, 2024.
	Ministry of Housing and Urban-Rural Development, State Administration for Market Regulation, Standard for lighting design of buildings: [S].Beijing: China Architecture & Building Press, 2024.
17	中国合格评定国家认可委员会. 实验室认可规则: CNAS—RL01 [S/OL]. (2019-12-30)[2025-02-15]. .
	China National Accreditation Service for Conformity Assessment. Rules for the Accreditation of Laboratories: CNAS-RL01 [S/OL]. (2019-12-30)[2025-02-15]. .
18	中国合格评定国家认可委员会. 检测和校准实验室能力认可准则:CNAS-CL01:2018 [S/OL]. (2019-02-20)[2025-02-15] .
	China National Accreditation Service Assessment. Accreditation criteria for the competence of testing and calibration laboratories:CNAS-CL01:2018 [S/OL]. (2019-02-20)[2025-02-15].

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