Abstract: Objective To select the effective microsatellite primers and analyze the genetic circumstance of individual Beagle dogs. Methods Twenty-four pair of primers with the heterozygotic degree between 0.4?0.8 were selected from the literature. The primers were authenticated used the mix-genome of 10 Beagle dogs. Results Among the 24 pair primers, 7 pair of primers were polymorphism and reproducibility. The 7 pair of primers used the mix-genome of 10 Beagle dogs of MaSi corporation were also tested，The results showed obviously different from Beagle dogs of our plant. The genetic circumstance of 6 individual Beagle dogs were also analyzed used the same 7 pair primers, four of them showed obviously different. Conclusion The 7 pair primers were suitable for analyzed the genetic circumstance of individual Beagle dogs primarily.

Key words: Beagle dogs, Closed colong, Microsatellite