Abstract: Objective To observe the fluctuation of uric acid in rat serum with hypoxanthine, uricase inhibitor, and to establish a new method to replicate rat hyperuricemia model. Method Adopted the combination of hypoxanthine and uricase inhibitor to establish rat hyperuricemia model, and compared the uric acid contents in serum when using hypoxanthine singly with that of using the combination of hypoxanthine and allantoxanic acid. Simultaneously those factors of research methods have been evaluated, such as those proposed model-leading drug’s dose-effect and time-effect relationship. Results The combination of hypoxanthine and uricase inhibitor could increase the content of uric acid in rat serum by means of appropriate dosage and method of administration (1000 )amol/L). It could create the rat hyperuricemia model successfully. Conclusion The combination of hypoxanthine and allantoxanic acid could increase uric acid contents in rats rather than using hypoxanthine singly. Meanwhile, by repeatedly dosing regimen，uric acid contents in serum of rats could increase during rather longer time (P<0.01). So it has made some progress in replicating steady hyperuricemia model at present experiment.

Key words: Hyperuricemia/gout, Hypoxanthine, Uricase inhibitor/ allantoxanic acid, Animal model