Abstract: Objective To establish a metastatic animal model of human glioblastoma by surgical tumor resection in nude mice, so as to provide an ideal animal model for experimental and clinical studies. Methods Histological intact tumor lesions of human glioblastoma U87MG were transplanted subcuta-neously into twenty-seven nude mice. When the tumor reached 20 mm in diameter, eighteen of these nude mice were randomly selected as experimental group and had their tumors resected to establish the postoperative metastatic animal model. The rest were assigned to the control group. All mice were sacrificed when the animals were obviously slender，the tumor metastasis rates and pathological changes were observed. Results The postoperative metastatic animal model of human glioblastoma were successfully established. In the experimental group，the average survival time of nude mice was 67.3 days, the average lung weight was 0.83士0.30 g，the metastasis rates in lung, lymph node, and cardia was 100% (18/18)，66.67% (12/18) and 5.56%(1/18), respectively. While in the control group, the average survival time of nude mice was 56 days，the metastasis rate of lung was 11.11% (1/9)，the average lung weight was 0.41±0.12 g. Conclusions This animal model simulates the metastasis after clinical operation, it is an ideal animal model for pathogenesis exploration and anti-metastasis treatment of human glioblastoma.

Key words: Human glioblastoma, Metastasis, Disease animal model, Tumor resection