Observation on Metastasis-related Genes of Highly Metastatic Human Lung Cancer Cell Line

doi:10.3969/j.issn.1674-5817.2011.02.001

Abstract

Abstract: Objective To observe the metastasis-associated genes expression in the highly metastatic human lung cancer cell line SPC-A-1sci and validate the highly metastatic characteristics of SPC-A-1sci in vivo. Methods The expression levels of several metastasis promoting genes concluding CD44, OPN, MMP-9, EGFR were analyzed between the high and low metastatic cell lines by RT-PCR, Western blotting, FICC, IHC and so on. The metastatic characteristics was investigated by injection the cell lines into the tail vein of nude mice. Results Compared with the low metastatic cell lines, the metastasis-associated genes CD44, OPN, MMP-9, EGFR in highly metastatic cells were significantly increased. After 8 weeks in vivo experiments, 100% (12/12) of mice injected with highly metastatic cell lines developed lung metastases, while low metastatic cell lines was 16.67%(2/12). Conclusions The metastasis-associated genes are highly expressed in SPC-A-1sci, and the SPC-A-1sci shows the typical characteristics of highly metastatic in vivo, and may be served as a reference for the application of the cell lines.

Key words: Human lung cancer, Cell lines, Cancer metastasis, Metastasis-associated genes

CLC Number:

R734.2Q95-33

YAO Ming, LI Jin, JIA De-shui, ZHANG Fan-lin, YAN Ming-xia, LIU Lei, ZHANG Shu-xia. Observation on Metastasis-related Genes of Highly Metastatic Human Lung Cancer Cell Line[J]. Laboratory Animal and Comparative Medicine, 2011, 31(2): 79-82.

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