Abstract: Objective To evaluate the effect of in vitro fertilization (IVF) on early embryo development and serum biochemistry in sexual maturity mouse. Methods The 2-cell embryos from IVF were transferred into pseudo-pregnant recipient mice. The fetus were weighed afler the embryo developed to 14 days (El4).Meanwhile，several pregnant mice were maintained until the pups were bom and weighed. The newborns were raised until sexual maturity, and the serum biochemistry of the mice was analyzed. Result The weight of E14 fetus from IVF group was 16.5% higher than that of the control group (P＜0.001)，the birth weight of IVF group was 13.2% higher than that of control group (P＜0.001)，but the differences of serum biochemistry in sexual maturity mouse between IVF group and control group were not significant. Conclusion The in vitro fertilization can affect the mouse early-embryo development, but it doesn’t affect the serum biochemistry in sexual maturity mouse.