Determination and Comparative Analysis of Hematological Indexes and Percentage of Lymphocyte Subsets in Several Immunodeficient Mice

doi:10.12300/j.issn.1674-5817.2020.123

Abstract

Abstract: Objective To determine and compare the hematological indexes (blood biochemical and physiological indexes) and the percentage of lymphocyte subsets in immunodeficient mice, providing basic data for tumor and immunology research applications. Methods (1) A total of 4 immunodeficient mouse strains, BALB/cA-nude, SCID, NOD-SCID, and NPSG, were included. Twenty mice (6-week-old, half male and half female) were analyzed. Blood biochemical (25 items) and physiological indexes (30 items) were determined and compared with those of BALB/cA-nude mice of the same age. (2) Four strains of immunodeficient mice, 4 mice (6-week-old, half male and half female) were randomly selected and analyzed with flow cytometry. Their symphocyte subsets were compared with those of BALB/cA-nude mice of the same age. Results (1) The white blood cell (WBC) count, lymphocyte (LYMPH) count, lymphocyte percentage (LYMPH%), and neutrophil count of immunodeficient mice were lower than those of BALB/cA mice (P<0.01). In contrast, the neutrophil percentage (NEUT%) and reticulocyte count were higher than those of BALB/cA mice (P<0.05, P<0.01). Furthermore, the levels of WBC, LYMPH and LYMPH% of SCID, NOD-SCID, and NPSG mice were significantly lower than those of BALB/cA-nude mice, while NEUT% was higher than that of BALB/cA-nude mice (P<0.05). (2) The blood glucose and triglyceride levels in NOD-SCID and NPSG mice were lower than those in BALB/cA mice (P<0.01), while uric acid level was higher than that of BALB/cA mice (P<0.05). (3) BALB/cA mice had no T cells and low activity of B cells and natural killer (NK) cells. SCID and NOD-SCID mice had no T cells and lower activity of B cells and NK cells than BALB/cA-nude mice. NPSG mice had no T, B, and NK cells. Conclusion The blood biochemical and physiological indexes and the percentage of blood lymphocyte subsets in BALB/cA-nude, SCID, NOD-SCID and NPSG mice are consistent with the characteristics of corresponding immunodeficient mouse strains.

Key words: Immunodeficient mice, Hematology, Serum biochemistry, Lymphocyte subsets

CLC Number:

Q95-33
R-332

TAN Xiaoqian, QU Wei, LI Liang, QIAN Zhen, GU Jianzhong, XU Ping. Determination and Comparative Analysis of Hematological Indexes and Percentage of Lymphocyte Subsets in Several Immunodeficient Mice[J]. Laboratory Animal and Comparative Medicine, 2021, 41(2): 155-160.

Figures/Tables 3

References

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