The Single Nucleotide Polymorphism Genotyping of Inbred Mice from Shanghai

doi:10.3969/j.issn.1674-5817.2017.01.006

Abstract

Abstract: Objective To analyze the single nucleotide polymorphism (SNP) genotyping of inbred mice from Shanghai. Methods 44 SNP loci on 21 chromosomes of 10 inbred mice strains from 2 laboratory animal companies of Shanghai were detected by the PCR-LDR genotyping panels, and the other 2 inbred mice strains were selected as the double blind samples for verification. And discrepancy locus were verified by sequencing. Results The SNP genotyping results of all the samples from the same inbred mice strains were homomorphism, but 6 inbred mice strains from different populations(BALB/c, FVB, DBA/2 and C3H/He) were polymorphism with 7 and 2 discrepancy locus respectively. The sequencing results to verify the discrepancy locus were the same as the SNP genotyping results. Conclusions The SNP genotyping was very stable and consistent in inbred mice strains from the same population in Shanghai, and the SNP genotyping of the same inbred mice strains from different populations was polymorphism.

Key words: Inbred mice, Genetic monitoring, Single nucleotide polymorphism (SNP) genotyping

CLC Number:

Q95-33

HAN Lin, XIE Jian-yun, YANG Fei, HU Ying, TIAN Li-li, BAO Shi-min. The Single Nucleotide Polymorphism Genotyping of Inbred Mice from Shanghai[J]. Laboratory Animal and Comparative Medicine, 2017, 37(1): 25-31.

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