Laboratory Animal and Comparative Medicine ›› 2016, Vol. 36 ›› Issue (4): 301-306.DOI: 10.3969/j.issn.1674-5817.2016.04.011

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Effects of Different Drugs and Disinfectants on Sporulation of Coccidian Oocysts in Rabbit

WEN Fu-li1, ZHANG Jia2, ZHENG He-ping1, MA Lei1, ZHANG Shi-lan1, WANG Shou-kun2   

  1. 1. Department of Comparative Medicine, Fuzhou General Hospital of Nanjing Command, PLA, Fuzhou 350025, China;
    2. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2016-05-30 Online:2016-08-25 Published:2016-08-25

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Abstract: Objective To study inhibitory effect of different drugs and disinfectants on coccidian oocyst sporulated in rabbit, and to provide reference for the prevention and treatment of rabbit coccidiosis. Methods Selected 5 kinds of drugs and disinfectant for gradient test, respectively. To observe their effects on sporulation process of rabbit coccidia oocysts in vitro. Results (1) Inhibition test was performed under 5 kinds of anticoccidial drugs.The drugs of inhibition rate for coccidian oocyst sporulated were shown in turns: Toltrazuril Solution > Decoquinate Premix > Dinitolmide Premix > Diclazuril Premix > Amprolium Hydrochloride and sulfaquioxaline Sodium. The median effect concentration (EC50) were shown as follows: Amprolium Hydrochloride and sulfaquioxaline Sodium > Diclazuril Premix > Dinitolmide Premix > Decoquinate Premix > Toltrazuril Solution. Take 15 mL/L Toltrazuril Solution effect of 8h has the strongest inhibitory on the sporulation of rabbit coccidian oocysts. (2) The rate of 5 kinds of disinfectants which inhibit rabbit coccidian oocyst from being sporulated was shown as follows:Carbon disulfide > Phenol > Ammonia > Symclosene > Tincture of iodine. The EC50 were shown as follows:Tincture of iodine > Symclosene > Ammonia > Phenol > Carbon disulfide. The hours for 50% of maximal effect (EH50) were shown as follows:Carbon disulfide > Phenol > Ammonia > Symclosene > Tincture of iodine. (3) The drug resistance of rabbit coccidiosis oocysts with strong toxicity were shown as follows: E.magna > E.stieda > E.irresidu > E.intestinal. Conclusions The 15 mL/L toltrazuril solution with effect of 8 h has the strongest inhibitory on the sporulation of rabbit coccidian oocysts. Carbon disulfide at concentration of 6% with effect of 4 h can completely inhibit rabbit coccidian oocysts from being sporulated. Drug resistance to rabbit coccidia oocysts was associated with body size of the parasite, and the larger body size with high sensitivity.

Key words: Rabbit, Coccidiosis, Anticoccidial drugs, Disinfectants, Sporulated oocysts

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