Abstract: Objective To evaluate the left ventricular diastolic function in healthy New Zealand White rabbits by multi-parameter echocardiography. To obtained normal values and analysis their correlation. Methods Left ventricular diastolic function can be measured by Pulsed Doppler, Tissue Doppler imaging and color M-mode Doppler. twenty-five rabbits were used in this study. Valves of mitral flow spectrum (E,A) and E/A were detected by Pulsed Doppler. Early and late diastolic peak velocity of mitral annulus (e’,a’) and e’/a’, E/e’ were measured by Tissue Doppler imaging (DTI). Isovolumic relaxation time (IVRT) was measured by Pulsed Doppler. Early diastolic flow propagation velocity (Vp) was measured by color M-mode Doppler. And we obtainednormal values of left ventricular diastolic function. Results The peak of E and A ,the peak e’ and a’ mixed together in three cases of all. Heart rate range was 180-324 beats/min. The normal values of left ventricular diastolic function were: E=0.44~1.05 m/s,A=0.23~0.47m/s,E/A=1.47~2.67.e’=6.96~19.92cm/s, a’=3.82~12.77cm/s, e’/a’=0.88~2.58, E/e’=2.70~8.80. IVRT=22.90~58.06 ms, Vp=38.90~81.52 respectively. There were significant correlation between E/A and e’/a’. No statistically significant among E/A, e’/a’, IVRT, Vp. Conclusions Left ventricular function of rabbits in cardiovascular disease can be evaluated by Pulsed Doppler and Tissue Doppler imaging.

Key words: Echocardiography, Multi-parameter, Diastolic function, Rabbit, Normal reference values