A New Mouse Model of Acute Hepatitis B Virus Infection

doi:10.3969/j.issn.1674-5817.2012.01.010

Abstract

Abstract: Objective To establish an acute HBV mouse model by tail vein injection of infectious sera. Methods Mice sera at 3 days after hydrodynamics-based transfection was collected and injected into the tail vein of BALB/c mice with volume of 0.1ml and 0.2ml respectively. The serum was slowly administrated within 10-12s. DNA levels of HBV e antigen (HBeAg), HBV surface antigen (HBsAg), HBV core antigen (HBcAg) and HBV were determined and compared before and after infectious sera injection. Results The levels of serum HBsAg, HBeAg, HBV DNA and liver HBcAg in mice injected with 0.1ml viral sera were higher than that injected with 0.2ml and hydrodynamic injected pBule-HBV. Conclusions The method of intravenous injection with low dose of infectious sera could be used to establish a mouse model of acute HBV infection.

Key words: Mouse model, Hepatitits B Virus, Infectious serum, Intravenous injection

CLC Number:

Q95-33

TANG Hong-bin, WU Kai-lang, CHENG La-ying, JIANG Ming-sen. A New Mouse Model of Acute Hepatitis B Virus Infection[J]. Laboratory Animal and Comparative Medicine, 2012, 32(1): 38-42.

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