Establishment of Atopic Dermatitis Mouse Model

doi:10.3969/j.issn.1674-5817.2018.04.005

Abstract

Abstract: Objective To explore a novel method for atopic dermatitis (AD) model in mouse induced by ovalbumin (OVA).Method The 6-weeks BALB/c female mice were subcutaneously injected with 0.5 mL of 200 µg/mL OVA solution on 1st day and subcutaneously injected with 0.5 mL of 100 µg/mL OVA solution on 6th day to induce systemic sensitization again.On 18th day and 39th day,1.5 cm² sterile gauze with 50 µL,1 000 µg/mL OVA solution were attached on the back of the mice and replaced every day for consecutive 7 days for the second local sensitization.On 46th day,the pathological changes in mouse skins were observed to judge whether mouse AD model was successfully established and the rates of being model and the mortality rate was calculated.Result The dermatitis changes,such as scab,flushing,desquamation,and so on,were shown in AD model mice.Also,the epidermal cells were thickened and the dermis was infiltrated with lymphocytes in AD model mice.The total serum IgE level in the AD model mice [(6 296.17±1 021.28) ng/Lsignificantly increased as compared with blank control mice [(1 280.97±501.24) ng/L](P<0.01) and the mice injected with saline [(1 327.57±629.71) ng/L(P<0.01).The rates of AD model and the mortality were respectively 94% and 6%.Conclusion Our study introduces a simple and stable method for mouse AD model induced by OVA.

Key words: Mouse model, Atopic dermatitis (AD), Ovalbumin (OVA)

CLC Number:

Q95-33

JI Lian, MA Tie, DI Zheng-hong, Liu Dong-yan. Establishment of Atopic Dermatitis Mouse Model[J]. Laboratory Animal and Comparative Medicine, 2018, 38(4): 267-271.

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