Survey Report Analysis on Parasitic and Microbial Quality of Laboratory Animals in Sichuan Province, 2017-2023

doi:10.12300/j.issn.1674-5817.2024.057

Abstract

Abstract:

Objective To provide reference for further strengthening the management of laboratory animals in Sichuan Province by reviewing and analyzing the results of parasitic and microbial quality inspections of laboratory animals from 2017 to 2023. Methods Sichuan Province has 31 licensed laboratory animal production units, with the main species including mice, rats, guinea pigs, rabbits, dogs, monkeys, and pigs. Sampling inspections and reports were conducted for units with laboratory animal production qualifications in accordance with current national and local standards for laboratory animal classification. The quality inspection results for various laboratory animals in Sichuan Province from 2017 to 2023 were analyzed. Results With the exception of 2018, annual quality inspections of laboratory animals were conducted every year between 2017 and 2023. Mice: In 2017, positive results for helminths, flagellates, Salmonella, murine hepatitis virus antibodies, and murine pneumonia virus antibodies were detected, with a pass rate of only 42.9%. In 2019, Staphylococcus aureus and Klebsiella pneumoniae were detected, with a pass rate of 86.7%. In 2021, Sendai virus antibodies were detected, yielding a pass rate of 85.7%.The pass rate in 2020, 2022, and 2023 was 100%. Rats: In 2017, positive results were found for helminths, mycoplasma antibodies, Staphylococcus aureus, Sendai virus antibodies, murine pneumonia virus antibodies, rat parvovirus RV strain antibodies, rat parvovirus (H-1) strain antibodies, and rat coronavirus antibodies, with a pass rate of 40.0%. In 2019, mycoplasma antibodies, Staphylococcus aureus and Klebsiella pneumoniae were detected, with a pass rate of 35.0%. No positive indicators were detected in 2020. In 2021, Sendai virus antibodies and rat parvovirus RV strain antibodies were detected, with a pass rate of 50.0%. In 2022, positive results for rat parvovirus RV strain antibodies were found, yielding a pass rate of 87.5%. In 2023, Pasteurella pneumotropica and Klebsiella pneumoniae were detected, with a pass rate of 85.7%. Dogs: In 2017 and 2019, the antibody titers for rabies virus and canine distemper virus were below the required standard, with pass rates of 33.3% and 20.0%, respectively. In 2020 and 2022, the pass rate was 100%. Guinea pigs (general grade): In 2019, positive results for Toxoplasma antibodies were detected, with a pass rate of 80.0%. In all other years, the pass rate was 100%. Monkeys: In 2019, positive results for Toxoplasma gondii and rhesus herpesvirus type I antibodies were found, with a pass rate of 87.5%.In 2020 and 2022, rhesus herpesvirus type I antibodies were positively detected, yielding pass rates of 93.3% and 97.5%, respectively. The pass rates in 2021 and 2023 were 100%. Clean guinea pigs, rabbits and pigs all passed the inspection each year. Conclusion Issues related to the parasitic and microbial quality of laboratory animals persist in Sichuan Province. Supervision and sampling inspections have proven to be effective in identifying these issues promptly, serving as a critical measure to ensure the quality of laboratory animals. The results of these inspections offer valuable data to support the healthy development of the laboratory animal industry in Sichuan Province.

Key words: Sichuan Province, Laboratory animals, Quality inspection, Parasites, Microorganisms

CLC Number:

S853.32

LIU Lida,CHEN Bing,XIE Na,et al. Survey Report Analysis on Parasitic and Microbial Quality of Laboratory Animals in Sichuan Province, 2017-2023[J]. Laboratory Animal and Comparative Medicine, 2024, 44(6): 654-660. DOI: 10.12300/j.issn.1674-5817.2024.057.

Figures/Tables 3

References 13

1	赵心刚, 卢凡, 程苹, 等. 我国实验动物资源建设的问题与展望[J]. 中国科学院院刊, 2019, 34(12):1371-1378. DOI: 10.16418/j.issn.1000-3045.2019.12.006 .
	ZHAO X G, LU F, CHENG P, et al. Problems and prospects of laboratory animal resources in China[J]. Bull Chin Acad Sci, 2019, 34(12):1371-1378. DOI: 10.16418/j.issn.1000-3045.2019.12.006 .
2	陈梅丽, 刘万策. 实验动物质量检测信息资源的建设与共享[J]. 实验动物科学, 2016, 33(1):33-37. DOI: 10.3969/j.issn.1006-6179.2016.01.008 .
	CHEN M L, LIU W C. Development and sharing of information resources for the quality inspection of laboratory animal[J]. Lab Anim Sci, 2016, 33(1):33-37. DOI: 10.3969/j.issn.1006-6179.2016.01.008 .
3	国家标准化管理委员会. 实验动物寄生虫学等级及监测: GB14922.1—2001[S]. 北京: 中国标准出版社, 2001.
	Standardization Administration of the People's Republic of China. Laboratory animal-Standards and monitoring for parasitology: GB 14922.1-2001[S]. Beijing: China Standards Press, 2001.
4	国家标准化管理委员会. 实验动物微生物学等级及监测: GB14922.2—2011[S]. 北京: 中国标准出版社, 2011.
	Standardization Administration of the People's Republic of China. Laboratory animal-Microbiological and parasitical standards and monitoring: GB 14922.2-2011[S]. Beijing: China Standards Press, 2001.
5	科学技术部. 实验动物微生物、寄生虫学等级及监测: [S]. 北京: 中国标准出版社, 2022.
	Ministry of Science and Technology of the People´s Republic of China. Laboratory animal-Microbiological and parasitical standards and monitoring: [S]. Beijing: China Standards Press, 2022.
6	四川省科学技术厅. 实验用猪微生物学和寄生虫学等级及监测: DB51/T 2757—2021[S/OL]. [2024-04-10]. https://dbba.sacinfo. org. cn/stdDetail/0646d899abc0e8232b57ebf3313f61884692e30fc4e333b4c770e8c8d2a9b559..
	Sichuan Provincial Department of Science and Technology. Experimental pig Standards and monitoring for microbiology and parasitology: DB51/T 2757-2021[S/OL]. [2024-04-10]. https://dbba. sacinfo. org. cn/stdDetail/0646d899abc0e8232b57ebf3313f61884692e30fc4e333b4c770e8c8d2a9b559.
7	李欣忆. 我省出台"十四五"生物经济发展规划生物经济规模到2025年超1.5万亿元[EB/OL]. (2021-10-07)[2024-04-02]. https://www. sc. gov. cn/10462/10464/10797/2021/10/7/f259500c38cb4a5896e8be7d8385196f.shtml.
	LI X Y. Our province issued the "14th Five-Year Plan" bioeconomy development plan, the scale of bioeconomy will exceed 1.5 trillion yuan by 2025[EB/OL]. (2021-10-07)[2024-04-02]. https://www. sc. gov. cn/10462/10464/10797/2021/10/7/f259500c38cb4a5896e8be7d8385196f.shtml.
8	陈兵, 邹弈星, 王敬东. 基于行政许可的四川省实验动物管理现状与对策分析[J]. 实验动物与比较医学, 2024, 44(5):560-566. DOI: 10.12300/j.issn.1674-5817.2024.055 .
	CHEN B, ZOU Y X, W J D. Analysis on current status and countermeasures for laboratory animal management in Sichuan Province based on administrative licensing[J]. Lab Anim Comp Med, 2024, 44(5):560-566. DOI:10.12300/j.issn.1674-5817.2024.055 .
9	刘丽达, 刘科亮, 何其励, 等. 2011—2015年四川省实验动物质量抽检结果分析[J]. 中国比较医学杂志, 2019, 29(2):102-105, 134. DOI: 10.3969/j.issn.1671-7856.2019.02.017 .
	LIU L D, LIU K L, HE Q L, et al. Results and analysis of sampling inspection for laboratory animal quality in Sichuan Province from 2011 to 2015[J]. Chin J Comp Med, 2019, 29(2):102-105, 134. DOI: 10.3969/j.issn.1671-7856.2019.02.017 .
10	魏杰, 黄健, 刘文菊, 等. 2017—2019年北京地区实验动物质量抽检结果分析[J]. 实验动物科学, 2021, 38(5):19-27, 58. DOI: 10.3969/j.issn.1006-6179.2021.05.005 .
	WEI J, HUANG J, LIU W J, et al. Analysis of random testing results of laboratory animals in Beijing Area from 2017 to 2019[J]. Lab Anim Sci, 2021, 38(5):19-27, 58. DOI: 10.3969/j.issn.1006-6179.2021.05.005 .
11	李晋文, 佟巍, 蔡鹃, 等. 猴B病毒抗体不同检测方法的比对[J]. 中国比较医学杂志, 2017, 27(7):29-33. DOI: 10.3969.j.issn.1671-7856.2017.07.006 .
	LI J W, TONG W, CAI J, et al. Comparison of different detection methods of monkey B virus antibody[J]. Chin J Comp Med, 2017, 27(7):29-33. DOI: 10.3969.j.issn.1671-7856.2017.07.006 .
12	蔡标, 戴陈伟, 李舜, 等. 实验猕猴B病毒抗体检测结果分析[J]. 安徽医学, 2018, 39(3):261-263. DOI: 10.3969/j.issn.1000-0399.2018.03.003 .
	CAI B, DAI C W, LI S, et al. Analysis on antibody testing of B virus(corcopithecine herpesvirus 1)[J]. Anhui Med J, 2018, 39(3):261-263. DOI: 10.3969/j.issn.1000-0399.2018.03.003 .
13	陈兵, 邹弈星, 姚凌云, 等. 四川省实验动物管理政策分析[J]. 实验动物与比较医学, 2024, 44(2): 209-213. DOI:10.12300/j.issn.1674-5817.2023.110 .
	CHEN B, ZOU Y X, YAO L Y, et al. Research on management policies of laboratory animals in Sichuan Province[J]. Lab Anim Comp Med, 2024, 44(2): 209-213. DOI:10.12300/j.issn.1674-5817.2023.110 .

年份 Year	抽检单位/家 Number of units	抽检动物数量/只^# Number of laboratory animals^#
年份 Year	抽检单位/家 Number of units	小鼠^a Mouse^a	大鼠^a Rat^a	豚鼠^b Guinea pig^b	豚鼠^c Guinea pig^c	兔^b Rabbit^b	犬^b Dog^b	猴^b Monkey^b	小型猪^b Miniature pig^b	总数 Total
2017	8	35 (7)	25 (5)	NA	10 (2)	NA	15 (3)	NA	NA	85
2019	13	30 (6)	20 (4)	10 (2)	10 (2)	10 (2)	10 (2)	40 (4)	NA	130
2020	9	25 (5)	25 (5)	NA	NA	NA	15 (3)	45 (3)	NA	110
2021	14	35 (7)	20 (4)	10 (2)	10 (2)	NA	NA	40 (4)	20 (4)	135
2022	17	50 (10)	40 (8)	NA	NA	NA	10 (2)	40 (4)	20 (4)	160
2023	14	50 (10)	35 (7)	10 (2)	10 (2)	5 (1)	NA	50 (4)	NA	160
Total	75	225	165	30	40	15	50	215	40	780

年份 Year	抽检单位/家 Number of units	抽检动物数量/只^# Number of laboratory animals^#
年份 Year	抽检单位/家 Number of units	小鼠^a Mouse^a	大鼠^a Rat^a	豚鼠^b Guinea pig^b	豚鼠^c Guinea pig^c	兔^b Rabbit^b	犬^b Dog^b	猴^b Monkey^b	小型猪^b Miniature pig^b	总数 Total
2017	8	35 (7)	25 (5)	NA	10 (2)	NA	15 (3)	NA	NA	85
2019	13	30 (6)	20 (4)	10 (2)	10 (2)	10 (2)	10 (2)	40 (4)	NA	130
2020	9	25 (5)	25 (5)	NA	NA	NA	15 (3)	45 (3)	NA	110
2021	14	35 (7)	20 (4)	10 (2)	10 (2)	NA	NA	40 (4)	20 (4)	135
2022	17	50 (10)	40 (8)	NA	NA	NA	10 (2)	40 (4)	20 (4)	160
2023	14	50 (10)	35 (7)	10 (2)	10 (2)	5 (1)	NA	50 (4)	NA	160
Total	75	225	165	30	40	15	50	215	40	780

动物 Animal	检出病原体 Pathogens		不同年份的检出阳性率/% Positive rate in different years/%
动物 Animal	类别 Category	名称 Name	2017	2019	2020	2021	2022	2023
SPF级小鼠 SPF mouse	寄生虫	蠕虫	17.1 (4a、2f)	0	0	0	0	0
		鞭毛虫	8.6 (3a)	0	NA	NA	0	0
	病原菌	沙门菌	5.7 (2d)	0	0	0	0	0
		金黄色葡萄球菌	0	10.0 (1b、2c)	0	NA	NA	NA
		肺炎克雷白杆菌	0	3.3 (1a)	0	0	NA	0
	病毒	小鼠肝炎病毒抗体	57.1 (5a、5c、5d、5f)	0	0	0	NA	0
		小鼠肺炎病毒抗体	20.0 (2a、5f)	0	0	0	0	0
		仙台病毒抗体	0	0	0	14.3 (5h)	0	0
SPF级大鼠 SPF rat	寄生虫	蠕虫	24.0 (1a、2c、3f)	0	0	0	0	0
	病原菌	支原体抗体	20.0 (5a)	25.0 (5h)	0	0	0	0
		金黄色葡萄球菌	16.0 (2b、2f)	35.0 (2b、5c)	0	NA	NA	NA
		嗜肺巴斯德杆菌	0	0	0	NA	NA	11.4 (4a)
		肺炎克雷白杆菌	0	5.0 (1a)	0	0	NA	2.9 (1l)
	病毒	仙台病毒抗体	4.0 (1a)	0	0	25.0 (5h)	0	0
		小鼠肺炎病毒抗体	20.0 (5a)	0	0	NA	0	NA
		大鼠细小病毒RV株抗体	48.0 (5a、5c、2f)	0	0	25.0 (5a)	13 (5a)	0
		大鼠细小病毒H-1株抗体	32.0 (4a、1c、3f)	0	0	NA	0	0
		大鼠冠状病毒抗体	20.0 (5a)	0	NA	0	NA	NA
普通级兔 Rabbit	寄生虫	弓形虫抗体	NA	0	NA	NA	NA	0
普通级犬 Dog	病毒	狂犬病毒抗体	40.0 (3c、3e、0g)	20.0 (1e、1g)	100	NA NA	100	NA NA
普通级犬 Dog	病毒	犬瘟热病毒抗体	73.3 (2c、5e、4g)	70 (3e、4g)	100	NA NA	100	NA NA
普通级豚鼠 Guinea pig	寄生虫	弓形虫抗体	NA	20.0 (2j)	NA	0	NA	0
普通级猴 Monkey	寄生虫	弓形虫抗体	NA NA	5.0 (1e、1i)	0	0	0	0
普通级猴 Monkey	病毒	猕猴疱疹病毒I型（B病毒）抗体	NA NA	12.5 (3e、2i)	6.7 (3e)	0	2.5 (1e)	0

动物 Animal	检出病原体 Pathogens		不同年份的检出阳性率/% Positive rate in different years/%
动物 Animal	类别 Category	名称 Name	2017	2019	2020	2021	2022	2023
SPF级小鼠 SPF mouse	寄生虫	蠕虫	17.1 (4a、2f)	0	0	0	0	0
		鞭毛虫	8.6 (3a)	0	NA	NA	0	0
	病原菌	沙门菌	5.7 (2d)	0	0	0	0	0
		金黄色葡萄球菌	0	10.0 (1b、2c)	0	NA	NA	NA
		肺炎克雷白杆菌	0	3.3 (1a)	0	0	NA	0
	病毒	小鼠肝炎病毒抗体	57.1 (5a、5c、5d、5f)	0	0	0	NA	0
		小鼠肺炎病毒抗体	20.0 (2a、5f)	0	0	0	0	0
		仙台病毒抗体	0	0	0	14.3 (5h)	0	0
SPF级大鼠 SPF rat	寄生虫	蠕虫	24.0 (1a、2c、3f)	0	0	0	0	0
	病原菌	支原体抗体	20.0 (5a)	25.0 (5h)	0	0	0	0
		金黄色葡萄球菌	16.0 (2b、2f)	35.0 (2b、5c)	0	NA	NA	NA
		嗜肺巴斯德杆菌	0	0	0	NA	NA	11.4 (4a)
		肺炎克雷白杆菌	0	5.0 (1a)	0	0	NA	2.9 (1l)
	病毒	仙台病毒抗体	4.0 (1a)	0	0	25.0 (5h)	0	0
		小鼠肺炎病毒抗体	20.0 (5a)	0	0	NA	0	NA
		大鼠细小病毒RV株抗体	48.0 (5a、5c、2f)	0	0	25.0 (5a)	13 (5a)	0
		大鼠细小病毒H-1株抗体	32.0 (4a、1c、3f)	0	0	NA	0	0
		大鼠冠状病毒抗体	20.0 (5a)	0	NA	0	NA	NA
普通级兔 Rabbit	寄生虫	弓形虫抗体	NA	0	NA	NA	NA	0
普通级犬 Dog	病毒	狂犬病毒抗体	40.0 (3c、3e、0g)	20.0 (1e、1g)	100	NA NA	100	NA NA
普通级犬 Dog	病毒	犬瘟热病毒抗体	73.3 (2c、5e、4g)	70 (3e、4g)	100	NA NA	100	NA NA
普通级豚鼠 Guinea pig	寄生虫	弓形虫抗体	NA	20.0 (2j)	NA	0	NA	0
普通级猴 Monkey	寄生虫	弓形虫抗体	NA NA	5.0 (1e、1i)	0	0	0	0
普通级猴 Monkey	病毒	猕猴疱疹病毒I型（B病毒）抗体	NA NA	12.5 (3e、2i)	6.7 (3e)	0	2.5 (1e)	0

[1]	LIU Yishu, CAI Liping. Advances and Challenges of Using Experimental Pigs in Da Vinci Surgical Robot Training [J]. Laboratory Animal and Comparative Medicine, 2024, 44(6): 667-674.
[2]	CHEN Bing, ZOU Yixing, WANG Jingdong. Analysis on Current Status and Countermeasures for Laboratory Animal Management in Sichuan Province Based on Administrative Licensing [J]. Laboratory Animal and Comparative Medicine, 2024, 44(5): 560-566.
[3]	ZHAO Yong. Three Dimensions of Animal Experiment Ethics: Analysis Based on Value of Life, Animal Welfare, and Risk Prevention [J]. Laboratory Animal and Comparative Medicine, 2024, 44(4): 445-454.
[4]	DENG Shaochang, LIN Danrong, LIANG Chujun, LEI Weiqiao, YANG Jinchun, ZHAO Weibo. Analysis of Institutional Characteristics and Implementation of Guangdong Province Laboratory Animals Ordinance in the Past Decade [J]. Laboratory Animal and Comparative Medicine, 2024, 44(4): 455-462.
[5]	SUN Qiang. History, Current Status, Challenges and Opportunities of Laboratory Monkey Industry in China [J]. Laboratory Animal and Comparative Medicine, 2024, 44(4): 343-356.
[6]	Lingzhi YU, Xiaofeng WEI, Ming LI, Zhihao KONG. Comparison of Methods between Soiled Bedding Sentinels and Exhaust Air Dust PCR for Health Monitoring of Rodent Laboratory Animals [J]. Laboratory Animal and Comparative Medicine, 2024, 44(3): 321-327.
[7]	Meitong LIU, Zhang CHEN, Zhaoqiang ZHANG, Di FAN, Zhan HU, Hailing MA. Formulation of Emergency Response Plan for Laboratory Animal Biosafety Emergencies in Hunan Province [J]. Laboratory Animal and Comparative Medicine, 2024, 44(3): 328-334.
[8]	Meitong LIU, Zhang CHEN, Zhaoqiang ZHANG, Di FAN, Zhan HU, Hailing MA. Investigation on Current Biosafety Management Status in Laboratory Animal Institutions in Hunan Province [J]. Laboratory Animal and Comparative Medicine, 2024, 44(2): 202-208.
[9]	Bing CHEN, Yixing ZOU, Lingyun YAO, Jingdong WANG. Research on Management Policies of Laboratory Animals in Sichuan Province [J]. Laboratory Animal and Comparative Medicine, 2024, 44(2): 209-213.
[10]	Yong ZHAO. Evolution and Prospects of Laboratory Animal Management: A Case Study of Shanghai's Development in the Past Decade [J]. Laboratory Animal and Comparative Medicine, 2023, 43(5): 492-503.
[11]	Lianxiang GUO. Revision of Standards for Microbiological and Parasitological Grades in Laboratory Animals and Its Comparison to Foreign Standards [J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 339-346.
[12]	Huan GOU, Xinying AN, Yujia TONG, Yan WANG, Shuang YANG. Analysis on the Development Status of Laboratory Animals in Japan [J]. Laboratory Animal and Comparative Medicine, 2023, 43(2): 194-204.
[13]	Chongyang QI, Lin CHEN, Man AI, Hang XU, Housen ZHANG. Safety Management Countermeasures Based on the Status of Laboratory Animal Administrative Licensing in Jiangsu Province [J]. Laboratory Animal and Comparative Medicine, 2023, 43(1): 79-85.
[14]	Xiaying LI, Yusheng WEI, Yonglu TIAN, Wanyong PANG. A Brief Interpretation of CCAC Guide on Humane Endpoints of Animals: 2022 Edition [J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 566-571.
[15]	Jian WANG, Jin LU, Lingyun TAO, Hongkun FU, Cheng GAO, Baojin WU, Yao LI, Yufeng TAO. Analysis on the Standardized Expression of the Accredited Ability Scope for Hematology, Blood Biochemistry and Urinalysis in Laboratory Animal Clinical Testing [J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 511-517.