Performance Test and Evaluation of Self-developed Biosafety Cage Changing Workbench

doi:10.12300/j.issn.1674-5817.2021.102

Abstract

Abstract:

Objective To test the function and evaluate the safety of the self-developed biosafety cage changing workbench.Methods According to the criteria of YY 0569-2011 "Class II Biological Safety Cabinet" and RB/T 199-2015 "Laboratory Equipment Biosafety Performance Evaluation Technical Specifications", we detected the various performance indicators of the cage changing workbench (HT-1303), which was independently developed and produced by Shenzhen Hongteng Biological Technology Co., LTD, and evaluated the safety and validity.Results The appearance integrity and stability of the main body of the biosafety cage changing workbench, the cleanliness of the working area, the velocity of the inflow airflow, the velocity of the descending airflow, the illuminance, the noise level, the airflow direction, the high-efficiency particulate air filter were at good condition, reaching the standards of level II biological safety cabinet. The airtightness of the contaminated goods recovery cabin and the sterilization chamber of utensil surface was good, but the size and quantity of the sterilized cage box need to be further improved.Conclusion The biosafety cage changing workbench developed by our team can be operated properly in whole. It can complete the replacement of individually ventilated cages in this workbench under negative pressure environment. Our workbench also can ensure the safety of operators, environment and experimental subjects.

Key words: Biosafety cage changing workbench, Performance test, Biosafety

CLC Number:

Q95-33

Shibo SU, Wenjie MA, Wei WANG, Huijuan GUO, He ZHANG, Zi WANG, Lei KANG, Shouqi LIU, Huairan LIU, Changde SI, Chuntian GOU, Wei ZHANG, Hongyan CHEN. Performance Test and Evaluation of Self-developed Biosafety Cage Changing Workbench[J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 102-110.

Figures/Tables 6

Figure1 Schematic diagram of self-developed biosafety mouse IVC cage changing workbench

Table 1 Number of particles missed of self-developed cage changer and some foreign cage changer and biosafety cabinet

检测项目	换笼台1	换笼台2	换笼台3	国外换笼台	安全柜
尘埃粒子浓度/ （10 $3$ 粒 $⋅$ L $- 1$ ）	51.55	51.70	51.56	51.69	51.39
尘埃粒子漏过量/ （粒 $⋅$ L $- 1$ ）	0.33	0.67	0.67	0.67	0.60

Table 1 Number of particles missed of self-developed cage changer and some foreign cage changer and biosafety cabinet

检测项目	换笼台1	换笼台2	换笼台3	国外换笼台	安全柜
尘埃粒子浓度/ （10 $3$ 粒 $⋅$ L $- 1$ ）	51.55	51.70	51.56	51.69	51.39
尘埃粒子漏过量/ （粒 $⋅$ L $- 1$ ）	0.33	0.67	0.67	0.67	0.60

Table 2 High efficiency filter leak detection of self-developed cage changer and some foreign cage changer and biosafety cabinet

检测项目	换笼台1	换笼台2	换笼台3	国外换笼台	安全柜
气溶胶浓度/ （μg·L $- 1$ ）	21.67	21.77	21.97	22.03	21.51
气溶胶漏过率/ ‱	0.08	0.07	0.07	0.07	0.10

Table 2 High efficiency filter leak detection of self-developed cage changer and some foreign cage changer and biosafety cabinet

检测项目	换笼台1	换笼台2	换笼台3	国外换笼台	安全柜
气溶胶浓度/ （μg·L $- 1$ ）	21.67	21.77	21.97	22.03	21.51
气溶胶漏过率/ ‱	0.08	0.07	0.07	0.07	0.10

Table 3 Gas flow rate of self-developed cage changer and some foreign cage changer and biosafety cabinet

检测项目	换笼台1	换笼台2	换笼台3	国外换笼台	安全柜
流入气流1	0.87	0.86	0.89	0.90	0.74
流入气流2	0.56	0.56	0.55	0.55	0.45
下降气流1	0.33	0.33	0.33	0.33	0.32
下降气流2	0.32	0.32	0.32	0.32	0.31
下降气流3	0.33	0.33	0.32	0.32	0.33

Table 4 Illuminance and noise value of self-developed cage changer and some foreign cage changer and biosafety cabinet

检测项目	换笼台1	换笼台2	换笼台3	国外换笼台	安全柜
背景照度/dB	324.00	320.25	320.00	324.50	342.00
照度/dB	715.00	733.00	738.50	718.75	760.00
背景噪声值/lx	53.67	53.67	54.33	53.33	55.00
噪声值/lx	64.00	64.33	65.33	65.33	66.00

Figure 2 Inspection chart of airflow pattern （A）， testing the air tightness of the contaminated goods recovery cabin （B） and the sterilization chamber of utensil surface （C） by soap bubble method

References 16

1	赵焱. 高级别生物安全实验室降低高致病性病毒实验生物安全防护水平的科研策略[J]. 病毒学报, 2021, 37(3):755-757. DOI:10.13242/j.cnki.bingduxuebao.003906 .
2	王宠, 郑琳琳, 王在峰. 生态环境监测实验室生物安全风险因子分析及防控措施[J]. 环境与发展, 2021, 33(2):157-164. DOI:10.16647/j.cnki.cn15-1369/X.2021.02.029 .
3	«实验动物与比较医学»编辑部. «中华人民共和国生物安全法»:病原微生物实验室生物安全[J]. 实验动物与比较医学, 2021, 41(5):465.
4	刘静, 李超, 柳金雄, 等. 高等级生物安全实验室在生物安全领域的作用及其发展的思考[J]. 中国农业科学, 2020, 53(1):74-80. DOI:10.3864/j.issn.0578-1752.2020.01.007 .
5	李鹏辉, 张宗兴, 徐新喜. 生物安全实验室初级防护设备和废弃物处理设备现状及发展建议[J]. 医疗卫生装备, 2021, 42(5):59-65. DOI:10.19745/j.1003-8868.2021100 .
6	张宗兴, 吴金辉, 衣颖, 等. 我国生物安全实验室关键防护技术与装备发展概况[J]. 中国卫生工程学, 2019, 18(5):641-646.
7	苏冠民, 孟蔚, 温宪芹, 等. 山东省Ⅱ级生物安全柜性能测试及分析[J]. 中国消毒学杂志, 2018, 35(11):872-873. DOI:10.11726/j.issn.1001-7658.2018.11.024 .
8	唐志坚, 赵生仓, 杨维雄, 等. 某单位Ⅱ级生物安全柜性能测试与分析[J]. 中国消毒学杂志, 2016, 33(8):723-725. DOI:10.11726/j.issn.1001-7658.2016.08.003 .
9	国家食品药品监督管理局. Ⅱ级生物安全柜: [S]. 北京: 中国标准出版社, 2013.
10	国家认证认可监督管理委员会. 实验室设备生物安全性能评价技术规范: RB/T 199—2015 [S]. 北京: 中国标准出版社, 2016.
11	中华人民共和国建设部. 生物安全柜: [S]. 北京: 中国标准出版社, 2005.
12	中华人民共和国国家质量监督检验检疫总局和中国国家标准化管理委员会. 实验室生物安全通用要求: 19489—2008 [S]. 北京:中国标准出版社, 2008.
13	中国国家认证认可监督管理委员会. 实验动物屏障和隔离装置评价通用要求: RB/T 010—2019 [S]. 北京: 中国标准出版社, 2020.
14	中华人民共和国住房和城乡建设部. 生物安全实验室建筑技术规范: [S]. 北京: 中国建筑工业出版社, 2012.
15	教霞, 刘越连, 杨舒筠, 等. 2019年疾控中心生物安全柜性能检测分析[J]. 中国药物与临床, 2020, 20(22):3838-3839. DOI:10.11655/zgywylc2020.22.062 .
16	杜丽雯. 生物安全柜高效过滤器完整性测试与分析[J]. 中国设备工程, 2020(4):157-159.

[1]	Wenjie MA, Wei ZHANG, Wei WANG, Shibo SU, Huijuan GUO, He ZHANG, Zi WANG, Huairan LIU, Hongyan CHEN. Performance Analysis of Class Ⅱ Biosafety Cabinetry in Veterinary-related Research Institutes [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 111-116.
[2]	Yong LI, Liang ZHOU, Yong HE, Zhifeng YAN, Haijiang WANG. Preliminary Exploration of Information Supervison System for Harmless Disposal of Laboratory Animal Carcasses and Related Wastes [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 122-126.
[3]	Lixiang CHEN, Boyin QIN, Hua YANG, Chunhua XU, Xiuhua PENG, Shun LI, Xiaohui ZHOU. Application Prospect of Identification and Traceability Technology of Laboratory Animals in Biosafety Laboratory [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 89-94.
[4]	Yuqin YANG. Biosafety Problems and Countermeasures in the Management of Barrier Facilities for Laboratory Animals [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 95-101.
[5]	CHEN Xiaojuan, LI Wei, WANG Lie. Exploration on Informatization of Laboratory Animal Management Process in Colleges and Universities: The Case of Zhejiang University [J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 554-558.
[6]	LUO Yinzhu, Min Fangui, WANG Jing, HE Lifang, PAN Jinchun. Prevention and Control of Pathogenic Microorganisms for the Management and Biosafety Control in Laboratory Animal Facilities [J]. Laboratory Animal and Comparative Medicine, 2021, 41(5): 443-449.
[7]	YANG Hua, ZHAO Yong, SONG Zhigang, LI Shun, ZHOU Xiaohui. A Brief Introduction About Key Points of Management in ABSL-2 Laboratory [J]. Laboratory Animal and Comparative Medicine, 2020, 40(2): 149-153.
[8]	ZHENG Mao-en, YANG Chun-hong, WANG Ke-zhou, LIU Tao, ZHAO Yuan-dong. Research Progress on Occupational Allergy of Laboratory Animal [J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 418-422.
[9]	XU Qing-hua, TONG Lin. Application of Analytic Hierarchy Process in Biosafety Management of Laboratory Animals [J]. Laboratory Animal and Comparative Medicine, 2018, 38(4): 299-303.
[10]	ZHAN Da-Wei-1, JIANG Qi-Hui-1, CHE Hong-2, HAO Zi-Qiang-3, SHANG Yu-Pu-1, HUANG Bin-1, SUN Yan-Song-1. Detection of Physical and Biological Parameters at Class Ⅱ Biosafety Cabinetry [J]. , 2006, 26(2): 94-97.