SLC24A5,SLC39A9基因在黑线仓鼠及其A: CHA品系表达水平的比较分析

doi:10.3969/j.issn.1674-5817.2014.06.002

实验动物与比较医学 ›› 2014, Vol. 34 ›› Issue (6): 438-442.DOI: 10.3969/j.issn.1674-5817.2014.06.002

SLC24A5,SLC39A9基因在黑线仓鼠及其A: CHA品系表达水平的比较分析

刘慧芳^1,2, 孙书芳³, 曾林², 曹玉华², 严翔², 范君文², 孙兆增², 王新¹

1.青岛农业大学,青岛 266109;
2.军事医学科学院实验动物中心,北京 100071;
3.山东省青岛市中心血站,青岛 266071

收稿日期:2014-05-22 出版日期:2014-12-25 发布日期:2014-12-25
作者简介:刘慧芳(1989-), 女, 硕士, 研究方向:中兽医学, E-mail:374278946@qq.com
基金资助:
国家自然科学基金项目(31272387)

Comparison of Expression Level of SLC24A5, SLC39A9 Genes in Cricetulus griseus and the Albino mutant

LIU Hui-fang^1,2, SUN Shu-fang³, ZENG Lin¹, CAO Yu-hua², YAN Xiang², FAN jun-wen², SUN Zhao-zeng², WANG Xin¹

1. Qingdao Agriculture University, Qingdao 266109,China;
2. Laboratory Animal Center of the Academy of Military Medical Science, Beijing 100071, China;
3. Qingdao Blood Bank, Qingdao 266071, China

Received:2014-05-22 Online:2014-12-25 Published:2014-12-25

摘要/Abstract

摘要： 目的比较可溶性物质运输蛋白SLC24A5(solute carrier family 24, member 5, SLC24A5)和SLC39A9(solute carrier family 39, member 9, SLC39A9)在黑线仓鼠及其白化突变系(A: CHA)皮肤组织的基因表达差异,分析这两种基因对白化性状产生的影响。方法提取黑线仓鼠和A: CHA的皮肤组织总RNA,利用普通PCR的方法筛选引物,利用实时荧光定量PCR技术,比较分析黑线仓鼠和A: CHA 皮肤组织SLC24A5、SLC39A9表达量的异同。结果黑线仓鼠皮肤中的SLC24A5、SLC39A9基因mRNA的相对表达量分别是A: CHA组织中的2倍和3.8倍。结论 SLC24A5、SLC39A9基因在A: CHA皮肤组织的表达量下调,说明SLC24A5和SLC39A9基因与A: CHA白化性状的产生存在一定的关联性。

关键词: 黑线仓鼠, A: CHA, SLC24A5, SLC39A9, 实时荧光定量PCR

Abstract: Objective To investigate the expression level of the soluble material transport protein SLC24A5 (solute carrier family 24, member 5, SLC24A5) and SLC39A9 (solute carrier family 39, member 9, SLC39A9) between the Cricetulus griseus and its albino mutation strain, A:CHA, and analyze the function on the albino phenotype. Methods The total mRNA of skin tissue in the Cricetulus griseus and A:CHA was extacted. The PCR method was used to select primers. The expression levels of SLC24A5, SLC39A9 was analyzed by real time PCR. Results The ratio of expression level of SLC24A5, SLC39A9 between Cricetulus griseus and A:CHA were 2 and 3.8 times. Conclusion The gene expression level of SLC24A5, SLC39A9 in the A:CHA were lower than that in the Cricetulus griseus. The study strongly supports the notion that the two genes may be contribute to the albino phenotype.

Key words: Cricetulus griseus, A:CHA, SLC24A5, SLC39A9, Real time quantitative PCR

中图分类号:

Q95-33

刘慧芳, 孙书芳, 曾林, 曹玉华, 严翔, 范君文, 孙兆增, 王新. SLC24A5,SLC39A9基因在黑线仓鼠及其A: CHA品系表达水平的比较分析[J]. 实验动物与比较医学, 2014, 34(6): 438-442.

LIU Hui-fang, SUN Shu-fang, ZENG Lin, CAO Yu-hua, YAN Xiang, FAN jun-wen, SUN Zhao-zeng, WANG Xin. Comparison of Expression Level of SLC24A5, SLC39A9 Genes in Cricetulus griseus and the Albino mutant[J]. Laboratory Animal and Comparative Medicine, 2014, 34(6): 438-442.

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SLC24A5,SLC39A9基因在黑线仓鼠及其A: CHA品系表达水平的比较分析

Comparison of Expression Level of SLC24A5, SLC39A9 Genes in Cricetulus griseus and the Albino mutant

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