洗洁精对鸡胚绒毛尿囊膜血管的刺激性评价

doi:10.3969/j.issn.1674-5817.2019.05.011

实验动物与比较医学 ›› 2019, Vol. 39 ›› Issue (5): 398-401.DOI: 10.3969/j.issn.1674-5817.2019.05.011

洗洁精对鸡胚绒毛尿囊膜血管的刺激性评价

陈梓灵, 徐颖愉, 刘香梅, 刘冬虹, 庞增雄

广州质量监督检测研究院, 广州 511447

收稿日期:2019-04-03 出版日期:2019-10-25 发布日期:2021-01-05
作者简介:陈梓灵, 女, 硕士研究生。研究方向:食品与化妆品安全性评价。E-mail: 107495302@qq.com

Study on Irritation of Dishwashing Liquid by Hen's Egg Test on Chorioallantotic Membrane

CHEN Zi-ling, XV Ying-yu, LIU Xiang-mei, LIU Dong-hong, PANG Zeng-xiong

Guangzhou Quality Supervison and Testing Instiute(GQT), Guangdong, Guangzhou 511447, China

Received:2019-04-03 Online:2019-10-25 Published:2021-01-05

摘要/Abstract

摘要： 目的探讨鸡胚尿囊膜试验(Het-Cam)在洗洁精刺激性评价中应用的可行性。方法将5款洗洁精样品配制成1.5%的水溶液,取100 μL滴加在SPF级鸡胚尿囊膜上,参照出入境检验检疫行业标准SN/T 2329-2009《化妆品眼刺激性/腐蚀性的鸡胚尿囊膜试验》的标准刺激评分(IS)法,对样品的刺激性进行评分。结果样品C和B刺激性评分较高,分别为10.07和8.84,而样品A和D刺激性评分较低,分别为5.69,6.75。上述4个样品相应的总活性物含量也分别为28.21%、15.34%和16.37%、17.14%。结论洗洁精刺激性与洗洁精中总活性物含量具有一定的相关性。Het-Cam在洗洁精刺激性评价方面既具有可行性。

关键词: 洗洁精, 鸡胚绒毛尿囊膜试验(Het-Cam), 刺激性

Abstract: Objective To explore the feasibility of hen's egg test on the chorioallantotic membrane (Het-Cam) in evaluating the irritation of dishwashing liquid. Methods Five dishwashing liquid samples were allocated into 1.5% solution, then add 100 μL to the chicken embryo allantoic membrane. Socred the irritation of the samples according to the irritation score (IS) of SN/T 2329-2009 Cosmetics ocular irritant and corrosive Het-Cam test. Results The irritation score of sample C and B are at the higher score for 10.07 and 8.84 respectively. The irritation score of sample A and D are at the lower score for 5.69 and 6.75 respectively. Their total active matter content are as follows: 15.34%,28.21%, 16.37% and 17.14%. Conclusion There is a certain correlation between the irritation of dishwashing liquid and the total active matter content in dishwashing liquid. Het-Cam test is not only feasible but also significant in evaluating the irritability of dishwashing liquid.

Key words: Sodium dodecyl sulfate, Hen's egg test on the chorioallantotic membrane (Het-Cam), Irritation

中图分类号:

Q95-33

陈梓灵, 徐颖愉, 刘香梅, 刘冬虹, 庞增雄. 洗洁精对鸡胚绒毛尿囊膜血管的刺激性评价[J]. 实验动物与比较医学, 2019, 39(5): 398-401.

CHEN Zi-ling, XV Ying-yu, LIU Xiang-mei, LIU Dong-hong, PANG Zeng-xiong. Study on Irritation of Dishwashing Liquid by Hen's Egg Test on Chorioallantotic Membrane[J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 398-401.

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洗洁精对鸡胚绒毛尿囊膜血管的刺激性评价

Study on Irritation of Dishwashing Liquid by Hen's Egg Test on Chorioallantotic Membrane

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