妊娠期L-NAME干预对子代大鼠学习认知能力的影响

›› 2009, Vol. 29 ›› Issue (4): 223-227.

妊娠期L-NAME干预对子代大鼠学习认知能力的影响

1.附属妇产科医院，上海，200011；2.实验动物科学部，上海，200032

收稿日期:2009-01-05 出版日期:2009-04-30 发布日期:2009-04-30

The Nitric Oxide Synthesis Inhibitor NW-Nitro-L-Arginie Methyl Ester(L-NAME) Causes Cognitive Ability Impairment in Offspring Rats

Fudan University 1. Hospital of Obstetrics and Gynecology, Shanghai 200011, China;2. Department of Laboratory Animal Science, Shanghai 200032, China

Received:2009-01-05 Online:2009-04-30 Published:2009-04-30

摘要/Abstract

摘要： 目的探讨妊娠期亚硝基左旋精氨酸甲酯（L-NAME）干预对子代大鼠学习认知能力的影响。方法通过给妊娠13 d大鼠连续7 d皮下注射L.NAME的方法建立妊娠期高血压疾病的大鼠模型，并根据剂量不同分为高剂量组（每天125 mg/kg）及低剂量组（每天75 mg/kg），对照组予生理盐水1 ml皮下注射。待自然分娩后，子代大鼠喂养至8周龄进行Morris水迷宫实验，分为定向导航试验研究智力和学习能力，空间探索试验研究记忆和空间定位能力。结果在妊娠期高血压疾病模型中，水迷宫定向导航试验第1天，高剂量组和低剂量组的逃避潜伏期（72.45±26.98 s，71.25±27.61 s）均明显长于对照组（56.07±20.30 s）（P＜0.05）；训练第4天，高剂量组逃避潜伏期（31.91±24.96 s）显著高于对照组（21.17±19.47 s）（P＜0.05）。在空间探索试验中，低剂量组子代大鼠在站台所在的IV象限路程百分组（26.65%±10.63%）显著低于对照组（33.73%±13.98%）（P＜0.05）。结论妊娠期L.NAME干预导致子代大鼠的智力受损，学习能力无明显差异。记忆和空间定位能力也有所降。

关键词: 妊娠期高血压疾病, 子代大鼠, L-NAME, 水迷宫, 学习认知能力

Abstract: Objective To explore the relationship between exposure to the nitric oxide synthesis inhibitor N①-nitro-L-arginine methyl ester (L-NAME) and the impairment of cognitive ability in offspring rats. Methods Maternal rats are randomly divided into 3 groups: (1) high dose group: maternal rats are subcutaneously injected with 125 mg/kg L-NAME in the 13th gestational day, and persisting in 7 days;(2) low dose group: maternal rats are subcutaneously injected with 75mg/kg L-NAME in the 13th gestational day，and persisting in 7 days;(3) control group: maternal rats are subcutaneously injected with 1 ml of 0.9% NaCl in the 13th gestational day, and persisting in 7 days. Afler the maternal rats is spontaneous delivery, the offspring rats are fed till they are 8 weeks old. The offspring rats were tested for visual-spatial learning and memory and spatial localization abilities in Morris water maze. Results In the animal model of preeclampsia, in the 1st day of place navigation test, the escape latent period of high dose group and low dose group is significant longer than that of control group (P＜0.05). In the 4th day, the escape latent period of high dose group is significant longer than that of control group (P＜0.05). In spatial exploration test, the percent of IV quadrant distance of low dose group is significant lower than that of control group(P＜0.05). Conclusion Exposure to the L-NAME results in the impairment of intelligence of the offspring rats. The study pattern has not significant difference. And the memory and space direction ability of offspring rats is impaired.

Key words: Hypertension disorder complicating pregnancy, offspring rat, L-NAME, Morris water maze, cognitive ability

朱蔚敏1，王春芳1，李笑天1，周光兴2，任芸芸1，潘华2. 妊娠期L-NAME干预对子代大鼠学习认知能力的影响[J]. , 2009, 29(4): 223-227.

ZHU Wei-min1，WANG Chun-fang1，LI Xiao-tian1，ZHOU Guang-xin2，REN Yun-yun1，PAN Hua2. The Nitric Oxide Synthesis Inhibitor NW-Nitro-L-Arginie Methyl Ester(L-NAME) Causes Cognitive Ability Impairment in Offspring Rats[J]. , 2009, 29(4): 223-227.

[1]	陈智玲, 吴华, 宋鑫磊. Ⅱ型糖尿病合并阿尔兹海默症小鼠模型的实验研究[J]. 实验动物与比较医学, 2018, 38(1): 29-35.
[2]	苗竞, 刘成全, 韩新民. 安神定志灵对注意力缺陷多动障碍模型大鼠自发活动、焦虑及空间学习能力的影响[J]. 实验动物与比较医学, 2014, 34(3): 210-213.
[3]	曾贵刚, 张申, 顾坚忠, 陈国强. SHR/SP与Wistar大鼠血管性痴呆模型的比较研究[J]. 实验动物与比较医学, 2012, 32(6): 461-466.
[4]	符鹏程，周琳，李旻，杨期东. ImageJ在Morris水迷宫视频行为分析中的应用[J]. , 2009, 29(3): 157-161.
[5]	刘艳霞1，胡晋红1，郑杰民2，朱全刚1，孙创斌1. 水迷宫水域调节方法的改良[J]. , 2008, 28(4): 243-245.