Table of Content

31 January 2009, Volume 29 Issue 1

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Variation of Myocardial Damage in Dilated Cardiomypathy Rabbit Model Induced by Adriamycin

MIAO Hai-wei1，LUO Xin-ping2，HUANG Cheng-lei2，WANG Yi-ping3，SUN Wei-kang3

2009, 29(1):  1-5. 

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Objective To observe the rule of variation myocardial damage in adriamycin-induced dilated cardiomyopathy (DCM ) in rabbit，and to determine the optimal time for further research. Methods Thrity-two rabbits were randomly assigned to control group(n=8) and model group (n=24). The DCM model was induced by adriamycin, 2 ml/kg body weight via ear vein for 8 weeks, the control group was given 2 ml/kg body weight of saline. Echocardiogram and hemodynamics parameters were assessed and plasma BNP was measuered at the 8th, 1 Oth and 12th week after the injections to model group ,and at 8th week to control group. Then the pathological change of the myocardium and microstructure were observed. Results The results of hemodynamics, ultrastructure and echocardiogram were coincident to the change of DCM. The myocardial damage occurred at 8th week in model group, and was most serious at 10th week, and lessened at 12th week (P＜0.05). Conclusions AT two weeks after the model established，the myocardial damage is most serious. It’s the optimal time for studying heart failure.

Sequence Analysis and Comparison on Olfactory Receptor Genes of German Shepherd Dog between Parents and the Filial Generation

MIAO Qin，ZHANG Hui-dong，GAO Yi-long

2009, 29(1):  6-11. 

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Objective To investigate the heredity the of olfactory receptor genes between the parents and the filial generation of German shepherd dog. Method Olfactory receptor genes of the parents and the filial generation of German shepherd dog were amplificated using the routine PCR method, and the related olfactory receptor genes were sequenced and analyzed. Result Olfactory receptor genes differed much more between the filial generation and the male parent than between the filial generation and the female parent. The T to C, G to C, A to G, C to T, G to T, T to A and G to A transitions were the main mutation type between parents and the filial generation. Some transitions resulting in the alteration in the protein sequence. Conclusion The results will be helpful to the research on the polymorphism and the heredity rule of canine olfactory receptor genes.

Research on Blood Lipid Regulating Effects of Soybean Isoflavones by Both apoE and LPL Gene Deficient (apoE-/-/LPL+/-) Mice

YU Xue-hui1，HAO Guo-xiang2，HU Pei-xin2，FAN Lin-lin2，LIU Chao1，ZHANG Cheng-mei1，ZHANG Shi-dong3

2009, 29(1):  12-16. 

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Objective To investigate the lipid regulating effects of soybean isoflavone extracts on genetic engineering mice with both hypercholesterolemia and hypertriglyceridemia. Method Under the western-type food conditioning，10(♂5，♀5) adult C57BL/6 mice were used as control, 40 adult mice with both aopE and LPL gene deficiency （apoE^-/-/LPL^+/-) were divided into four groups, i.e., the group of animal models of hyperlipidemia，three groups of intragastric administration respectively with dose of 200 mg·kg^-1·d^-1 of soybean isoflavone extracts，0.5 mg·kg^-1 of nylestrodial per week and 20 mg·kg^-1·d^-1 of lovastatin. After four weeks consecutive administration, concentration of serum total cholesterol, triacylglycerol，HDL cholesterol，LDL cholesterol and glucose were determined. Result Compared with the control mice of C57BL/6，the diet induced models of hyperlipidemia had a remarkable increase in triglyceride，total and LDL cholesterol(P<0.01). Compared with models of hyperlipidemia, administration of soybean isoflavones had a favorable reduction in triglyceride (p<0.05) and a 9.3% increase in HDL cholesterol, while administration of nylestrodial and lovastatin had 13.5% and 8.7% reduction in HDL cholesterol respectively. Probably due to the greatly increased total and LDL cholesterol in apoE^-/-/LPL^+/- mice, there is no effects of soybean isoflavone, nylestrodial and lovastatin on both the variables. Conclusion Soybean isoflavones had evident favorable lipid regulating effects.

Study on Original Culture Condition of Rabbit Embryonic Stem Cell

ZHANG Hui1，FANG Zhen-fu2，KUANG Ling1，CHEN Xue-jin2

2009, 29(1):  17-21. 

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Objective To investigate the effect of mouse embryonic endoderm cells and chick embryo extract(CEE) on the primary culture of rabbit embryonic stem cell, Methods A new method have been used to study the establishment of rabbit embryonic stem cell line. The condition systems were made up by feeder and medium. Mouse embryonic endoderm cells were inactivated by mitomycin c as feeder. Some different mediums were tested in this system. The inner cell masses separated from rabbit blastocyst were put into this condition system. After the inner cell mass attached to the feeder layer and formed outgrowth，the outgrowth with embryonic stem cell’s typical morphology were passaged. Results In the system consisted of mouse embryonic endoderm cells inactivated as feeder, 78% D-MEM/F-12+20% Knockout SR+2 mmol/L L-Glutamine+1 %MEM NAA+0.1 mmol/L p -mercapto ethanol+8 ng/ml hrbFGF+2%CEE as medium, the rabbit inner cell masses could grew into outgrowth with embryonic stem cell’s typical morphology. Conclusions Endoderm cells maybe were better than embryonic fibroblast for rabbit embryonic stem cell growth, and CEE maybe contained some growth factor which could inhibit rabbit embryonic stem cell differentiation.

Study on Experimental Infection of TTV in NEW Zeland Rabbits

CHEN Yan-ping，LIN Zhi-ming，WANG Chang-qi，ZHU Jin-ping

2009, 29(1):  22-24. 

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Objective To explore the sensitivy of rabbits to TTV infection. Methods Twelve rabbits were divided into three groups as three generations used fot infectious passag experiment of TTV. Rabbits of the first generation were infected with positive serum of TTV, the second generation were infected with serum from the first generation and those of the third generation with serum taken from the second generation. Result Various degrees of biochemical alteration in serum and pathological changes in liver were found in all experimental rabbits. By nest-PCR, detection on the serum of third generation showed positive. Three of the rabbits were positive with Dig-TTV DNA probe and other one negative. The nucleotide sequence analysis of the positive samples of strain(AB008394) CHI and JX strain TTV, showed the homology 96.7%, 98.3%, and 99.7% respectively, Conclusion Rabbits can be infected with TTV, and are sensitive animals for establishment of an animal model.

Changes of Oxygen Free Radicals in Pancreata of Rabbits

LI Cun-zhao，LI Yong，HUANG Qun

2009, 29(1):  25-27. 

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Objectives To study whether sodium nitroprusside may protect the ischemia-reperfusion injury in pancreata by measuring oxygen free radicals following hepatic blood-supply blockage in rabbits with portal hypertension. Method Twenty four rabbits were randomly divided into three groups, which were treated with sodium nitroprusside (S)，N-nitro-L-arginine methylester (L), and normal solution (C) respectively. Oxygen free radicals in pancreatic tissues, which had been taken 60 minutes after reperfusion following portal vein occlusion, were measured. Results Compared with group C, group S had no significant changes in nitric oxide synthase (NOS), but had significantly increased nitric oxide (NO), superoxide dismutase (SOD), and adenosine-triphosphatase (ATPase), and had remarkably reduced malondialdehyde (MDA). By contrast, compared with those in group C, NO, SOD, NOS, and ATPase in group L were significantly reduced, and MDA was obviously increased. Conclusion Sodium nitroprusside protects the ischemia-reperfusion injury in pancreata after hepatic blood-supply blockage.

Application of Bombyx mori as Model Organism in Modern Biology

ZHU Xiao-su，SONG yan，XU Shi-qmg

2009, 29(1):  61-65. 

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Silkworm，i.e. Bombyx mori, as the typical representative of lepidopteran insect and the ideal biological model，has played a significant role in promoting the development of animal science, which specific mutation has already been the optimum material for the research on modern genetic engineering. With the accomplishment of the draft of the silkworm genome and the fine map, silkworm will further become a comparatively ideal model organism in the field of research on life sciences in postgenome era. This text summarized the silkworm’s contributions to the modem genetics; again, analyzed the status quo of silkworm as the model organism in researching realms of developmental biology, bioreactor, and the construction of molecular platform of genome and so forth; and finally, discussed the application of silkworm being the model organism in the field of Modem Biology.

Progress on Animal Models for Non-insulin-dependent Diabetes Mellitus

LI Na, QU Hui, CAO Yu-li, ZHANG Zhou

2009, 29(1):  66-66. 

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Human diabetic mellitus is the complicated metabolic disease, especially of non-insulin-dependent diabetes mellitus (NIDDM). Then the appropriate diabetic mellitus animal model is the important basic of research. There are many NIDDM animal models, such as experimental NIDDM animal model，spontaneous NIDDM animal model, transgenic NIDDM animal model and anti-insulin cell model for NIDDM, and so on. This mini-review mainly focused on the advances of the animal models, which from the pathogenesis and clinic symptom,and also the prospect was indicated.