Laboratory Animal and Comparative Medicine

In Vivo Gene Transfer and Expression in Mice with Eukaryotic Expression Vector Containing Human mUK cDNA

CUI Ning-1, HAN Qin-Qin-1, CHEN Hao-Zhu-1, LIN Bing-2, SONG Hou-Yan-2

1995, 15(4): 193-197.

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The possibility of treating thrombosis diseases by gene transfer was studied with eukaryotic expression vector containing human mUK cDNA. Thirty Kunming mice were divided into 6 groups. The doses (ip) of pcDNA 3 mUK in group 1—4 were 20, 40, 60, 80 pig/mouse respectively. The other 2 groups were injected intraperitoneally with pcDNA 3 and transfection solution respectively as controls. The plasma mUK activity was measured with synthetic—peptide substrate S 2390. They increased significantly (P＜0.05) in group 2-4 from the 5th to the 60th day after the injection as compared with those before the injection (940±200 IU/L, n=30), and reached the peaks of 2200±220, 3050±340，3350±260 IU/L respectively 10 days after the injection. The result suggests that the method of injection with recombinant plasmid containing human mUK cDNA may be applied to the prevention and treatment of thrombosis diseases.

Changes of Endothelin Contents in Plasma and Tissues Induced by Acute Hypoxia in Rats

ZHOU Zheng-Mou, ZHAO Yun-Tao, SUN A-Cheng, SHI Xiang-Yun, LANG De-Juan, YU Song-Hai

1995, 15(4): 198-200.

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The concentrations of endothelin were measured by radioimmunoassay in 15 rats after being placed in low atmospheric pressure (36.971 kPa) hypoxia for 30 min to mimic the flying altitude of 7500 m. Compared with the control, the concentration of plasma endothelin of the experimental group increased markedly (P＜0.001)> and the concentrations of endothelin in lung, right atrial myocardium and thalamus decreased significantly (P＜0.001). The results suggest that the synthesis, release and metabolism of the endothelin in the experimental subjects developed serious disturbance by acute hypoxia, which may be significant in the damages of the body in hypoxia.

Effects of ⁶⁰Co-γ Radiation on Feed for Rats and Mice

ZHANG Zhou-1, ZHANG Su-Yin-1, YANG Qiu-Long-1, MAO Jia-Xian-2

1995, 15(4): 201-205.

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To determine the effective sterilizing dose of ⁶⁰Co-γ ray for rat and mouse feed, the granulated feed was radiated with ⁶⁰Co-γ ray in different doses. The result showed that the effective dose was 25 kGy. The storage term of the radiated feed was at least 90 d. When the feed was radiated with a dose of 30 kGy, its storage term exceeded 180 d. No marked distruction of the nutrients of the radiated feed was found except vitman B₁ which decreased about 20% , while in the autoclaved (121℃, 30 min) feed the crude protein, amino acids and vitmans decreased, especially the vitman B₁ decreased about 50%. The weanling nude mice fed with the radiated feed (25 kGy) for one month grew faster than those fed with the autoclaved feed (P＜0.05).

Histopathological Observation and DNA Image Analysis on Livers of HBV Transgenic Mice

WAN Shu-Guang-1, LI Yan-Feng-1, ZHANG Ping-Ping-1, WAN Da-Fang-1, QIAN Geng-Sun-1, GU Jian-Ren-1, CHENG Guo-Xiang-2, XU Shao-Fu-2

1995, 15(4): 206-209.

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"The histopathological observation on the livers of thirty two ICR/JCL HBV transgenic mice at 23—55 days old revealed that in 17 mice there were enlarged hepatocytes with round, oval or irregularly shaped nucleus, thick nuclear membrane, coarse and dark-stained chromatin, enlarged nucleoli and increased nucleus-cytoplasmic ratio. The structure of liver lobules was essentially normal. The proportion of such abnormal cells was relatively high in 7 of the 17 mice, but there were no typical pathological changes of hepatitis in these cases. Analysis of DNA images showed that there was an increase in DNA content of the abnormal hepatocytic nuclei. The proportion of the cells with the number of chromosomes over penta-ploid reached 12%—56%. "

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