Application Analysis of Animal Models for Pelvic Inflammatory Disease Based on Data Mining

doi:10.12300/j.issn.1674-5817.2024.012

Abstract

Abstract:

Objective To investigate the key elements for model establishment and determine the evaluation indicators of animal models for pelvic inflammatory disease (PID), providing a reference for improving modelling methods and optimizing the application of PID animal models. Methods The search query "Pelvic Inflammatory Disease" AND "Animal Model" OR "Rat" OR "Mouse" OR" Guinea Pig" OR "Rabbit" OR "Dog" OR "Pig" was used to retrieve relevant literature on PID animal models published from 2013 to 2023 in China Knowledge Network Infrastructure (CNKI), Wanfang, and PubMed databases. The studies were analyzed and categorized based on experimental animal types, modelling methods, modelling cycles, detection indicators, positive control drugs, and administration duration. A database was established for statistical analysis. Results A total of 214 research articles on PID animal models meeting the inclusion criteria were identified. The most commonly used model animals are Sprague Dawley (SD) rats, followed by Wistar rats. The most frequently employed modelling method is a combination of mechanical injury and bacterial infection, followed by the phenol mucilage method. The most common modelling cycles for acute pelvic inflammatory disease (APID) and chronic pelvic inflammatory disease (CPID)/sequelae of pelvic inflammatory disease (SPID) are 8 to 14 days, while for PID models without specific staging, the cycles are 7 days. High-frequency detection methods and indicators include histopathological observation using hematoxylin-eosin staining, enzyme-linked immunosorbent assay (ELISA) for serum-related indicators, morphological changes of tissues observed with the naked eye, and immunohistochemical detection of related protein expression in uterine tissues, and pathological scoring. The most frequently used positive control drugs are Fuke Qianjin Tablets, followed by Jingangteng Capsules. The most common administration duration for APID is 7 days, and for CPID/SPID models, it ranges from 15 to 21 days. Conclusion Currently, SD rats and Wistar rats are commonly used as experimental animals for PID models. The dual modelling method of mechanical injury combined with mixed bacterial infection aligns closely with clinical pathogenesis and can be used to establish a PID model that simulates postoperative uterine cavity infection. Depending on the research objectives, different positive drugs and detection indicators should be selected for comprehensive evaluation. Most existing PID animal model studies are based on western medical diagnosis, with fewer studies focusing on Traditional Chinese Medicine (TCM) syndromes. There is a need to integrate TCM theories of etiology and pathogenesis to construct PID animal models that are more in line with TCM clinical symptoms.

Key words: Data mining, Pelvic inflammatory disease, Animal models, Application analysis, Rats

CLC Number:

R-332

ZHENG Yiqing,DENG Yasheng,FAN Yanping,et al. Application Analysis of Animal Models for Pelvic Inflammatory Disease Based on Data Mining[J]. Laboratory Animal and Comparative Medicine, 2024, 44(4): 405-418. DOI: 10.12300/j.issn.1674-5817.2024.012.

Figures/Tables 6

References 82

1	谢幸, 孔北华, 段涛. 妇产科学[M]. 9版. 北京: 人民卫生出版社, 2018: 251-257.
	XIE X, KONG B H, DUAN T. Obstetrics and gynaecology[M]. 9th ed. Beijing: People's Health Press, 2018:251-257.
2	SAVARIS R F, FUHRICH D G, MAISSIAT J, et al. Antibiotic therapy for pelvic inflammatory disease[J]. Cochrane Database Syst Rev, 2020, 8(8):CD010285. DOI: 10.1002/14651858.CD010285.pub3 .
3	魏绍斌. 中医药防治盆腔炎性疾病及其后遗症的思路、方法及长期管理策略[J]. 北京中医药大学学报, 2023, 46(9):1204-1212. DOI: 10.3969/j.issn.1006-2157.2023.09.003 .
	WEI S B. Thoughts, methods, and long-term management strategies of traditional Chinese medicine in preventing and treating pelvic inflammatory diseases and their sequelae[J]. J Beijing Univ Tradit Chin Med, 2023, 46(9):1204-1212. DOI: 10.3969/j.issn.1006-2157.2023.09.003 .
4	周瑶瑶, 白明华, 李竹青, 等. 从中医体质学理论探讨女性盆腔炎性疾病的三级预防方案[J]. 中华中医药杂志, 2023, 38(1):243-246.
	ZHOU Y Y, BAI M H, LI Z Q, et al. Discussion on the three-level prevention scheme of female pelvic inflammatory disease from the theory of TCM constitution[J]. China J Tradit Chin Med Pharm, 2023, 38(1):243-246.
5	苗明三. 实验动物和动物实验技术[M]. 北京: 中国中医药出版社, 1997.
	MIAO M S. Laboratory animals and animal experimental techniques[M]. Beijing: China Press of Traditional Chinese Medicine, 1997.
6	黄慧, 邓亚胜, 梁天薇, 等. 卵巢储备功能减退动物模型的造模方法评价与分析[J]. 实验动物与比较医学, 2023, 43(4):422-428. DOI: 10.12300/j.issn.1674-5817.2023.032 .
	HUANG H, DENG Y S, LIANG T W, et al. Evaluation and analysis of modeling methods for animal models with diminished ovarian reserve[J]. Lab Anim Comp Med, 2023, 43(4):422-428. DOI: 10.12300/j.issn.1674-5817.2023.032 .
7	张岩雪, 李红艳, 孙军华, 等. 化瘀固本中药方对慢性盆腔炎大鼠盆腔粘连及TGF-β1/Smads信号通路的影响[J]. 中国优生与遗传杂志, 2022, 30(7):1162-1167. DOI: 10.13404/j.cnki.cjbhh.2022.07.030 .
	ZHANG Y X, LI H Y, SUN J H, et al. Effects of Huayu Guben Chinese herbal formula on pelvic adhesion and TGF-β1/Smads signaling pathway in rats with chronic pelvic inflammation[J]. Chin J Birth Health Hered, 2022, 30(7):1162-1167. DOI: 10.13404/j.cnki.cjbhh.2022.07.030 .
8	乔文艳, 邓克红, 张娟, 等. 基于TGF-β/Smads通路研究芍药苷对慢性盆腔炎大鼠的抗纤维化和抗炎作用[J]. 中医药信息, 2022, 39(5):45-50. DOI: 10.19656/j.cnki.1002-2406.20220508 .
	QIAO W Y, DENG K H, ZHANG J, et al. Anti-fibrosis and anti-inflammation of paeoniflorin in treating CPID rats based on TGF-β/smads pathway[J]. Inf Tradit Chin Med, 2022, 39(5):45-50. DOI: 10.19656/j.cnki.1002-2406.20220508 .
9	FAN L Y, LIU Z H, ZHANG Z, et al. Identifying the clinical presentations, progression, and sequela of pelvic inflammatory disease through physiological, histological and ultrastructural evaluation of a rat animal model[J]. Ann Transl Med, 2021, 9(23):1710. DOI: 10.21037/atm-21-3345 .
10	刘小月, 黄海涛, 卜晓玲. 参芪扶正汤对盆腔炎性疾病后遗症小鼠上生殖道炎症的影响[J]. 中医学报, 2021, 36(12):2615-2619. DOI: 10.16368/j.issn.1674-8999.2021.12.542 .
	LIU X Y, HUANG H T, BU X L. Effect of Shenqi fuzheng decoction on inflammatory of upper reproductive tract in mice with sequelae of pelvic inflammatory disease[J]. Acta Chin Med, 2021, 36(12):2615-2619. DOI: 10.16368/j.issn.1674-8999.2021.12.542 .
11	黄凤珍, 田贵华, 刘敏, 等. 细菌和激素共暴露建立大鼠盆腔炎模型的方法[J]. 绿色科技, 2018(8):222-226. DOI: 10.16663/j.cnki.lskj.2018.08.080 .
	HUANG F Z, TIAN G H, LIU M, et al. Simple establishment method of rat models of acute and chronic pelvic inflammation diseases[J]. J Green Sci Technol, 2018(8):222-226. DOI: 10.16663/j.cnki.lskj.2018.08.080 .
12	邓蒂斯, 黄叶芳, 龚道银. 银甲片对盆腔炎性疾病大鼠模型炎性因子及输卵管纤毛形态的影响研究[J]. 成都中医药大学学报, 2022, 45(2):69-74. DOI: 10.13593/j.cnki.51-1501/r.2022.02.069 .
	DENG D S, HUANG Y F, GONG D Y. Study of Yinjia Tablet regulating inflammatory factors and oviductal Cilia morphology in rats with pelvic inflammatory disease[J]. J Chengdu Univ Tradit Chin Med, 2022, 45(2):69-74. DOI: 10.13593/j.cnki.51-1501/r.2022.02.069 .
13	刘薇, 黄精俸, 江振洲, 等. 宁泌泰胶囊对大鼠大肠杆菌感染性盆腔炎的改善作用研究[J]. 药物评价研究, 2015, 38(6):612-616. DOI: 10.7501/j.issn.1674-6376.2015.06.006 .
	LIU W, HUANG J F, JIANG Z Z, et al. Experiment research on therapeutic effect of Ningmitai Capsule for pelvic inflammation[J]. Drug Eval Res, 2015, 38(6):612-616. DOI: 10.7501/j.issn.1674-6376.2015.06.006 .
14	刘莉, 李宗云, 刘志荣, 等. 黄芪甲苷对慢性盆腔炎大鼠子宫的保护作用及机制[J]. 中药药理与临床, 2023, 39(9):38-43. DOI: 10.13412/j.cnki.zyyl.20230404.003 .
	LIU L, LI Z Y, LIU Z R, et al. Protective effect and mechanism of astragaloside IV on uteri of rats with chronic pelvic inflammatory disease[J]. Pharmacol Clin Chin Mater Med, 2023, 39(9):38-43. DOI: 10.13412/j.cnki.zyyl.20230404.003 .
15	王英军, 孙英莲. 金丹平炎胶囊对慢性盆腔炎模型大鼠输卵管通畅率及炎性因子表达的影响[J]. 特产研究, 2021, 43(4):49-52. DOI: 10.16720/j.cnki.tcyj.2021.044 .
	WANG Y J, SUN Y L. The effect of jindanpingyan capsule on tubal patency and expression of inflammatory factors in chronic pelvic inflammatory disease model rats[J]. Spec Wild Econ Anim Plant Res, 2021, 43(4):49-52. DOI: 10.16720/j.cnki.tcyj.2021.044 .
16	肖萍, 林彩霞, 盘冰洁, 等. 岩黄连栓治疗慢性盆腔炎大鼠的药效学研究[J]. 中南药学, 2019, 17(12):2052-2058. DOI: 10.7539/j.issn.1672-2981.2019.12.008 .
	XIAO P, LIN C X, PAN B J, et al. Pharmacodynamics of Yanhuanglian suppository for rats with chronic pelvic inflammatory diseases[J]. Cent South Pharm, 2019, 17(12):2052-2058. DOI: 10.7539/j.issn.1672-2981.2019.12.008 .
17	徐阳美, 宋洋洋, 任弋, 等. 妇平胶囊对小鼠慢性盆腔炎模型的治疗作用研究[J]. 临床合理用药杂志, 2017, 10(5):15-18. DOI: 10.15887/j.cnki.13-1389/r.2017.05.008 .
	XU Y M, SONG Y Y, REN Y, et al. Study of the treatment effect of Fuping capsule on chronic pelvic inflammatory disease mice[J]. Chin J Clin Ration Drug Use, 2017, 10(5):15-18. DOI: 10.15887/j.cnki.13-1389/r.2017.05.008 .
18	马慧敏, 杨丽红, 金瑞林, 等. 慢性盆腔炎模型大鼠中miR-29及炎症信号通路分子的表达水平及其作用机制研究[J]. 现代检验医学杂志, 2022, 37(6):14-18, 109. DOI: 10.3969/j.issn.1671-7414.2022.06.003 .
	MA H M, YANG L H, JIN R L, et al. Study on the expression level and mechanism of miR-29 and inflammatory signal pathway molecules in chronic pelvic inflammatory disease model rats[J]. J Mod Lab Med, 2022, 37(6):14-18, 109. DOI: 10.3969/j.issn.1671-7414.2022.06.003 .
19	孙兰, 李家春, 王燕, 等. 18 F-FDG MicroPET技术评价桂枝茯苓胶囊对大鼠盆腔炎的抗炎作用[J]. 中成药, 2022, 44(11):3482-3488. DOI: 10.3969/j.issn.1001-1528.2022.11.013 .
	SUN L, LI J C, WANG Y, et al. Evaluation of anti-inflammatory effects of Guizhi Fuling Capsules on pelvic inflammatory disease in rats using 18 F-FDG MicroPET imaging system[J]. Chin Tradit Pat Med, 2022, 44(11):3482-3488. DOI: 10.3969/j.issn.1001-1528.2022.11.013 .
20	张锁, 贾瑞林, 陈晶. 薏苡附子败酱散加味对盆腔炎性疾病后遗症模型大鼠JNK/p38信号通路表达影响[J]. 中华中医药杂志, 2022, 37(6):3584-3589.
	ZHANG S, JIA R L, CHEN J. Effects of modified Yiyi Fuzi Baijiang Powder on JNK/p38 signaling pathway expression in rats with pelvic inflammatory disease sequelae[J]. China J Tradit Chin Med Pharm, 2022, 37(6):3584-3589.
21	肖志葵, 谭知浩, 罗弘杉, 等. 基于转录组学探讨夏枯草茎叶总多酚对慢性盆腔炎大鼠的药效学作用及机制[J]. 特产研究, 2023, 45(2):81-89. DOI: 10.16720/j.cnki.tcyj.2023.044 .
	XIAO Z K, TAN Z H, LUO H S, et al. Pharmacodynamic effect and mechanism of Prunella vulgaris stem and leaf total polyphenols on chronic pelvic inflammatory disease in rats based on transcriptomics[J]. Spec Wild Econ Anim Plant Res, 2023, 45(2):81-89. DOI: 10.16720/j.cnki.tcyj.2023.044 .
22	梁照, 鲁秋丹, 金哲. 丹枝饮对盆腔炎性疾病后遗症小鼠体内炎性因子的影响[J]. 天津中医药大学学报, 2017, 36(1):33-37. DOI: 10.11656/j.issn.1673-9043.2017.01.09 .
	LIANG Z, LU Q D, JIN Z. Effect of Danzhi Decoction on serum levels of inflammatory factors in murine model with sequelae of pelvic inflammatory disease[J]. J Tianjin Univ Tradit Chin Med, 2017, 36(1):33-37. DOI: 10.11656/j.issn.1673-9043.2017.01.09 .
23	蒲茜, 余蕾. 脐带间充质干细胞对于盆腔炎性后遗症大鼠的治疗作用及机制初探[J]. 中国计划生育和妇产科, 2022, 14(8):85-90. DOI: 10.3969/j.issn.1674-4020.2022.08.21 .
	PU Q, YU L. Therapeutic effect and mechanism of umbilical cord mesenchymal stem cells in rats with pelvic inflammatory sequelae[J]. Chin J Fam Plan Gynecotokology, 2022, 14(8):85-90. DOI: 10.3969/j.issn.1674-4020.2022.08.21 .
24	赵杰, 刘芳. 千金片对慢性盆腔炎大鼠Th1/2型细胞因子表达的影响[J]. 中国老年学杂志, 2017, 37(1):32-34. DOI: 10.3969/j.issn.1005-9202.2017.01.013 .
	ZHAO J, LIU F. Effect of Qianjin Tablet on expression of Th1/2 cytokines in rats with chronic pelvic inflammatory disease[J]. Chin J Gerontol, 2017, 37(1):32-34. DOI: 10.3969/j.issn.1005-9202.2017.01.013 .
25	刘俊宇, 曾元莲, 秦旭华, 等. 肿节风抗炎镇痛及对盆腔炎模型大鼠的影响[J]. 中药药理与临床, 2022, 38(3):135-140. DOI: 10.13412/j.cnki.zyyl.2022.03.024 .
	LIU J Y, ZENG Y L, QIN X H, et al. Anti-inflammatory and analgesic effect of SARCANDRAE HERBA and its influence on rat model of pelvic inflammation[J]. Pharmacol Clin Chin Mater Med, 2022, 38(3):135-140. DOI: 10.13412/j.cnki.zyyl.2022.03.024 .
26	张忠, 苏红宁, 刘姣, 等. 坤舒康颗粒抗大鼠棉球肉芽肿形成及其作用机制[J]. 中国医药导报, 2017, 14(2):12-15.
	ZHANG Z, SU H N, LIU J, et al. Inhibitive effect and mechanism of Kunshukang Granules on the formation of cotton ball granuloma in rats[J]. China Med Her, 2017, 14(2):12-15.
27	木则帕尔·太来提, 阿尼克孜·阿不都艾尼, 赛米热·艾斯拉. 当归芍药散对慢性盆腔炎模型大鼠免疫状态及NF-κB信号通路的影响[J]. 中国计划生育学杂志, 2022, 30(3):505-508. DOI: 10.3969/j.issn.1004-8189.2022.03.003 .
	MUZEPAL. T, ANANIZ. A, SEMIGE. I.Effects of Danggui Shaoyao San on immune status and NF-κB signal pathway of model rats with chronic pelvic inflammato-ry disease[J]. Chin J Fam Plan, 2022, 30(3):505-508. DOI: 10.3969/j.issn.1004-8189.2022.03.003 .
28	谷风, 沈祖泓, 谷周蓉, 等. 利湿化瘀法对湿瘀型慢性盆腔炎大鼠子宫组织NF-κBp65、IκKα蛋白表达影响的研究[J]. 中国中医药科技, 2018, 25(4):481-484.
	GU F, SHEN Z H, GU Z R, et al. Effects of removing dampness and blood stasis method on NF-κBp65 and IκKα protein expressions in uterine tissue of chronic pelvic inflammatory rats[J]. Chin J Tradit Med Sci Technol, 2018, 25(4):481-484.
29	杨静, 易刚, 王磊, 等. 甲连盆腔胶囊对盆腔炎性疾病后遗症大鼠的保护作用及机制探讨[J]. 现代中西医结合杂志, 2020, 29(20):2167-2173. DOI: 10.3969/j.issn.1008-8849.2020.20.001 .
	YANG J, YI G, WANG L, et al. Protective effect and mechanism of Jialian pelvic capsule on sequelae of pelvic inflammatory disease in rats[J]. Mod J Integr Tradit Chin West Med, 2020, 29(20):2167-2173. DOI: 10.3969/j.issn.1008-8849.2020.20.001 .
30	谷风, 曾远强, 谷周蓉, 等. 利湿化瘀法对湿瘀型慢性盆腔炎大鼠子宫组织Ⅰ型胶原纤维及IkKβ影响的研究[J]. 中国中医药科技, 2018, 25(6):808-811. DOI：CNKI:SUN:TJYY.0.2018-06-011 .
	GU F, ZENG Y Q, GU Z R, et al. Effects of removing dampness and stasis method on collagen type Ⅰ fibers and IκKβ expressions in uterus of rats with chronic pelvic inflammatory[J]. Chin J Tradit Med Sci Technol, 2018, 25(6):808-811. DOI： CNKI:SUN:TJYY.0.2018-06-011 .
31	王阳, 刘小月, 卜晓玲. 参芪扶正汤对盆腔炎性疾病后遗症小鼠上生殖道细胞自噬水平的影响[J]. 河北中医药学报, 2022, 37(2):1-5, 9. DOI: 10.16370/j.cnki.13-1214/r.2022.02.005 .
	WANG Y, LIU X Y, BO X L. Effect of Shenqi fuzheng decoction on autophagy level of upper reproductive tract cells in mice with sequelae of pelvic inflammatory disease[J]. J Hebei Tradit Chin Med Pharmacol, 2022, 37(2):1-5, 9. DOI: 10.16370/j.cnki.13-1214/r.2022.02.005 .
32	易丽贞, 刘欣, 吴雪芬, 等. 妇科千金片对盆腔炎大鼠TLR4、PI3K、AKT表达的影响[J]. 中国处方药, 2021, 19(1):31-34. DOI: 10.3969/j.issn.1671-945X.2021.01.017 .
	YI L Z, LIU X, WU X F, et al. Effect of Fuke Qianjin Tablets on TLR4, PI3K and AKT in pelvic inflammatory model rats[J]. J China Prescr Drug, 2021, 19(1):31-34. DOI: 10.3969/j.issn.1671-945X.2021.01.017 .
33	杨伟娜, 杨军娜, 姚伊. 五味消毒饮对急性盆腔炎模型大鼠JAK2/STAT3信号通路及炎性因子的影响[J]. 中医学报, 2019, 34(10):2138-2143. DOI: 10.16368/j.issn.1674-8999.2019.10.496 .
	YANG W N, YANG J N, YAO Y. Effects of Wuwei Xiaodu drink on JAK2/STAT3 signaling pathway and inflammatory factors in rats with acute pelvic inflammation[J]. Acta Chin Med, 2019, 34(10):2138-2143. DOI: 10.16368/j.issn.1674-8999.2019.10.496 .
34	杨伟娜, 杨军娜, 姚伊. 桃核承气汤通过JAK2/STAT1信号通路干预急性盆腔炎模型大鼠的研究[J]. 中国医院用药评价与分析, 2019, 19(9):1075-1078, 1082. DOI: 10.14009/j.issn.1672-2124.2019.09.010 .
	YANG W N, YANG J N, YAO Y. Research of the intervention of taohechengqi decoction on model rats with acute pelvic inflammatory disease through JAK2/STAT1 signal pathway[J]. Eval Anal Drug Use Hosp China, 2019, 19(9):1075-1078, 1082. DOI: 10.14009/j.issn.1672-2124.2019.09.010 .
35	岳秀永, 秦建设, 方应权, 等. 表没食子儿茶素没食子酸酯对慢性盆腔炎大鼠子宫组织的影响[J]. 中成药, 2018, 40(5):1182-1184. DOI: 10.3969/j.issn.1001-1528.2018.05.038 .
	YUE X Y, QIN J S, FANG Y Q, et al. Effect of epigallocatechin gallate on uterine tissue in rats with chronic pelvic inflammatory disease[J]. Chin Tradit Pat Med, 2018, 40(5):1182-1184. DOI: 10.3969/j.issn.1001-1528.2018.05.038 .
36	王霞, 桑飞, 郭鑫, 等. 赤芍总苷对慢性盆腔炎大鼠炎症抑制作用及NF-κB通路的调节作用研究[J]. 河北医药, 2022, 44(6):805-809. DOI: 10.3969/j.issn.1002-7386.2022.06.001 .
	WANG X, SANG F, GUO X, et al. Study on the inhibitory effects of total glucosides of paeony on inflammation and the regulation effects on NF-κB pathway in rats with chronic pelvic inflammation[J]. Hebei Med J, 2022, 44(6):805-809. DOI: 10.3969/j.issn.1002-7386.2022.06.001 .
37	陈晓强, 陆惠玲, 石乂丹, 等. 电针对盆腔炎性疾病后遗症大鼠子宫组织环氧合酶-2和转化生长因子-β1表达的影响[J]. 中华中医药杂志, 2022, 37(8):4688-4691.
	CHEN X Q, LU H L, SHI Y D, et al. Effects of electroacupuncture on expression of cyclooxygenase 2 and transforming growth factor-β1 in uterine tissue of sequelae of peivic inflammatory disease rats[J]. China J Tradit Chin Med Pharm, 2022, 37(8):4688-4691.
38	LI Y, LIU Y, YANG Q, et al. Anti-inflammatory effect of feiyangchangweiyan capsule on rat pelvic inflammatory disease through JNK/NF-κB pathway[J]. Evid Based Complement Alternat Med, 2018, 2018:8476147. DOI: 10.1155/2018/8476147 .
39	TANG P, DING Q, LIN J, et al. Pen Yan Jing tablets alleviates pelvic inflammatory disease by inhibiting akt/NF-κB pathway[J]. Int J Med Sci, 2023, 20(11):1386-1398. DOI: 10.7150/ijms.87433 .
40	WANG C M, LA L, FENG H X, et al. Aldose reductase inhibitor engeletin suppresses pelvic inflammatory disease by blocking the phospholipase C/protein kinase C-dependent/NF-κB and MAPK cascades[J]. J Agric Food Chem, 2020, 68(42):11747-11757. DOI: 10.1021/acs.jafc.0c05102 .
41	KONG D J, FU P, ZHANG Q, et al. Protective effects of Asiatic acid against pelvic inflammatory disease in rats[J]. Exp Ther Med, 2019, 17(6):4687-4692. DOI: 10.3892/etm.2019.7498 .
42	BU X L, LIU Y X, LU Q D, et al. Effects of "Danzhi decoction" on chronic pelvic pain, hemodynamics, and proinflammatory factors in the murine model of sequelae of pelvic inflammatory disease[J]. Evid Based Complement Alternat Med, 2015, 2015:547251. DOI: 10.1155/2015/547251 .
43	张岩雪, 李红艳, 孙军华, 等. 基于PD-1/PD-L1信号通路探讨妇炎汤对慢性盆腔炎小鼠Treg/Th17免疫平衡的影响[J]. 中国优生与遗传杂志, 2022, 30(5):744-749. DOI: 10.13404/j.cnki.cjbhh.2022.05.002 .
	ZHANG Y X, LI H Y, SUN J H, et al. Based on PD-1/PD-L1 signaling pathway investigate the influence of Fuyan Decoction on Treg/Th17 immune balance[J]. Chin J Birth Health Hered, 2022, 30(5):744-749. DOI: 10.13404/j.cnki.cjbhh.2022.05.002 .
44	LI Y, YANG Q, SHI Z H, et al. The anti-inflammatory effect of Feiyangchangweiyan capsule and its main components on pelvic inflammatory disease in rats via the regulation of the NF-κB and BAX/BCL-2 pathway[J]. Evid Based Complement Alternat Med, 2019, 2019:9585727. DOI: 10.1155/2019/9585727 .
45	罗娜, 孙蔚林, 宁静, 等. 骨髓间充质干细胞来源外泌体对盆腔炎模型大鼠氧化应激和炎症反应的影响[J]. 西部医学, 2022, 34(5):681-687. DOI: 10.3969/j.issn.1672-3511.2022.05.010 .
	LUO N, SUN W L, NING J, et al. Effects of exosomes derived from bone marrow mesenchymal stem cells on oxidative stress and inflammatory response in rats with pelvic inflammatory disease[J]. Med J West China, 2022, 34(5):681-687. DOI: 10.3969/j.issn.1672-3511.2022.05.010 .
46	宗利平, 侯思伟, 郝明玲, 等. 千金苇茎汤合桃红四物汤对慢性盆腔炎模型大鼠的影响[J]. 中医学报, 2023, 38(1):145-151. DOI: 10.16368/j.issn.1674-8999.2023.01.026 .
	ZONG L P, HOU S W, HAO M L, et al. Effect of Qianjin Weijing Decoction and Taohong Siwu Decoction on chronic pelvic inflammation model rats[J]. Acta Chin Med, 2023, 38(1):145-151. DOI: 10.16368/j.issn.1674-8999.2023.01.026 .
47	江利, 姜梦婕, 韩克. 桂枝茯苓丸对慢性盆腔炎大鼠血清炎症因子水平及子宫组织caspase-3、caspase-8表达的影响[J]. 中成药, 2021, 43(10):2846-2850. DOI: 10.3969/j.issn.1001-1528.2021.10.046 .
	JIANG L, JIANG M J, HAN K. Effect of Guizhi Fuling pill on serum inflammatory factor level and expression of caspase-3 and caspase-8 in uterine tissue of rats with chronic pelvic inflammation[J]. Chin Tradit Pat Med, 2021, 43(10):2846-2850. DOI: 10.3969/j.issn.1001-1528.2021.10.046 .
48	邹学红, 汪俊, 王芳, 等. 金刚藤颗粒对急性盆腔炎模型大鼠NLRP3炎症小体通路及免疫功能的影响[J]. 中国中医急症, 2019, 28(8):1362-1365, 1382. DOI: 10.3969/j.issn.1004-745X.2019.08.012 .
	ZOU X H, WANG J, WANG F, et al. Effects of Jingangteng Granule oEffects of Jingangteng Granule on NLRP3 inflammatory body pathway and immunologic function of in rats with acute pelvic inflammation[J]. J Emerg Tradit Chin Med, 2019, 28(8):1362-1365, 1382. DOI: 10.3969/j.issn.1004-745X.2019.08.012 .
49	李茂雅, 魏绍斌, 黄利. 经PBEF/Caspase-3通路研究妇炎舒胶囊治疗盆腔炎性疾病模型大鼠的作用机制[J]. 中华中医药学刊, 2022, 40(9):128-131. DOI: 10.13193/j.issn.1673-7717.2022.09.029 .
	LI M Y, WEI S B, HUANG L. Mechanism of fuyanshu capsules in treatment of pelvic inflammatory disease model rats via PBEF/caspase-3 pathway[J]. Chin Arch Tradit Chin Med, 2022, 40(9):128-131. DOI: 10.13193/j.issn.1673-7717.2022.09.029 .
50	谷风, 谷周蓉, 由春玲, 等. 利湿化瘀中药对慢性盆腔炎大鼠输卵管组织Fas/FasL通路的影响[J]. 中国中医药科技, 2020, 27(4):515-519.
	GU F, GU Z R, YOU C L, et al. Effects of removing dampness and blood stasis Chinese medicine on fas/FasL pathway of fallopian tubal tissue of rats with chronic pelvic inflammatory[J]. Chin J Tradit Med Sci Technol, 2020, 27(4):515-519.
51	ZHANG L J, ZHU J Y, SUN M Y, et al. Anti-inflammatory effect of Man-Pen-Fang, a Chinese herbal compound, on chronic pelvic inflammation in rats[J]. J Ethnopharmacol, 2017, 208:57-65. DOI: 10.1016/j.jep.2017.06.034 .
52	齐进, 崔颖娜. 金刚藤多糖对慢性盆腔炎大鼠炎症介质、细胞凋亡及免疫细胞功能的影响[J]. 海南医学院学报, 2018, 24(13):1219-1221, 1225. DOI: 10.13210/j.cnki.jhmu.20180515.002 .
	QI J, CUI Y N. Effects of smilax bockii warb polysaccharide on inflammatory mediators, apoptosis and immune cell function in rats with chronic pelvic inflammatory disease[J]. J Hainan Med Univ, 2018, 24(13):1219-1221, 1225. DOI: 10.13210/j.cnki.jhmu.20180515.002 .
53	刘凤萍, 张民英. 妇乐颗粒对家兔盆腔炎模型炎症细胞因子及 ICAM-1的影响[J]. 陕西中医, 2015, 36(8):1090-1091. DOI: 10.3969/j.issn.1000-7369.2015.08.075 .
	LIU F P, ZHANG M Y. Effect of Fule Granule on inflammatory cytokines and ICAM-1 in rabbit pelvic inflammatory disease model[J]. Shaanxi J Tradit Chin Med, 2015, 36(8):1090-1091. DOI: 10.3969/j.issn.1000-7369.2015.08.075 .
54	秦翠梅, 于洪建, 陈建梅, 等. 三棱-莪术有效组分配伍液对慢性盆腔炎大鼠盆腔粘连的影响[J]. 中成药, 2018, 40(6):1233-1237. DOI: 10.3969/j.issn.1001-1528.2018.06.001 .
	QIN C M, YU H J, CHEN J M, et al. Effects of active components in compatible solution of Sparganii Rhizoma-Curcumae Rhizoma on pelvic adhesion in rats with chronic pelvic inflammatory disease[J]. Chin Tradit Pat Med, 2018, 40(6):1233-1237. DOI: 10.3969/j.issn.1001-1528.2018.06.001 .
55	谷风, 陶红星, 苗久旺, 等. 泽丹冲剂对盆腔炎性大鼠子宫 TGF-β1mRNA 、CTGFmRNA的干预研究[J]. 陕西中医, 2014, 35(5):623-625. DOI: 10.3969/j.issn.1000-7369.2014.05.062 .
	GU F, TAO H X, MIAO J W, et al. Intervention study of Zedan Granule on TGF-β1mRNA and CTGFmRNA in uterus of rats with pelvic inflammatory disease[J]. Shaanxi J Tradit Chin Med, 2014, 35(5):623-625. DOI: 10.3969/j.issn.1000-7369.2014.05.062 .
56	李筠, 陈刚. 金刚藤胶囊对慢性盆腔炎大鼠盆腔粘连的作用及其药理机制[J]. 中药药理与临床, 2020, 36(1):144-149. DOI: 10.13412/j.cnki.zyyl.2020.01.021 .
	LI J, CHEN G. Effect and pharmacological mechanism of Jingangteng capsule on pelvic adhesion in rats with chronic pelvic inflammation[J]. Pharmacol Clin Chin Mater Med, 2020, 36(1):144-149. DOI: 10.13412/j.cnki.zyyl.2020.01.021 .
57	安琪, 郑建华. 康妇消炎栓对盆腔炎大鼠子宫VEGF、EGF和MUC-1水平的影响[J]. 基因组学与应用生物学, 2018, 37(9):4200-4207. DOI: 10.13417/j.gab.037.004200 .
	AN Q, ZHENG J H. Effect of Kangfu Xiaoyan suppository on the levels of VEGF, EGF and MUC-1 in uterus of rats with pelvic inflammatory disease[J]. Genom Appl Biol, 2018, 37(9):4200-4207. DOI: 10.13417/j.gab.037.004200 .
58	吕耀中, 宗绍波, 李芳, 等. 散结镇痛胶囊对慢性盆腔炎大鼠抗炎及抗纤维化研究[J]. 中草药, 2019, 50(20):5011-5017. DOI: 10.7501/j.issn.0253-2670.2019.20.024 .
	LÜ Y Z, ZONG S B, LI F, et al. Anti-inflammatory and anti-fibrosis effects of Sanjie Zhentong Capsule on chronic pelvic inflammatory disease in rats[J]. Chin Tradit Herb Drugs, 2019, 50(20):5011-5017. DOI: 10.7501/j.issn.0253-2670.2019.20.024 .
59	许浩, 丁渊. 红藤煎剂对盆腔炎模型大鼠子宫内膜整合素αvβ3表达影响的实验研究[J]. 中国中医药科技, 2017, 24(2):159-160, 184. DOI：CNKI:SUN:TJYY.0.2017-02-012 .
	XU H, DING Y. Experimental study of Hongteng Decoction on endometrial integrin αvβ3 expression of rats with pelvic inflammation[J]. Chin J Tradit Med Sci Technol, 2017, 24(2):159-160, 184. DOI：CNKI:SUN:TJYY.0.2017-02-012 .
60	许浩, 丁渊. 红藤煎剂对盆腔炎大鼠子宫内膜HOXA10表达影响的实验研究[J]. 中国中医药科技, 2016, 23(6):668-670. DOI：CNKI:SUN:TJYY.0.2016-06-014 .
	XU H, DING Y. Experimental study on effect of Hongteng Decoction on endometrial HOXA10 expression of rats with pelvic inflammation[J]. Chin J Tradit Med Sci Technol, 2016, 23(6):668-670. DOI：CNKI:SUN:TJYY.0.2016-06-014 .
61	张秋慧, 匡继林, 李萍. 盆炎方对盆腔炎性疾病后遗症大鼠卵巢、子宫组织TGF-β1、TβRⅡ蛋白的影响[J]. 上海中医药杂志, 2016, 50(2):84-88. DOI: 10.16305/j.1007-1334.2016.02.026 .
	ZHANG Q H, KUANG J L, LI P. The influence of "Penyan Fang" on the TGF-β1 and TβR Ⅱ expression of the sequelae of pelvic inflammatory disease rats' ovary and uterus[J]. Shanghai J Tradit Chin Med, 2016, 50(2):84-88. DOI: 10.16305/j.1007-1334.2016.02.026 .
62	张志鹏, 高升, 任存霞. 当归芍药散对慢性盆腔炎模型大鼠分子免疫调控的影响[J]. 中华中医药学刊, 2015, 33(11):2684-2686. DOI: 10.13193/j.issn.1673-7717.2015.11.037 .
	ZHANG Z P, GAO S, REN C X. Effect of Danggui Shaoyao Powder on molecular immune regulation of chronic pelvic inflammatory disease model rats[J]. Chin Arch Tradit Chin Med, 2015, 33(11):2684-2686. DOI: 10.13193/j.issn.1673-7717.2015.11.037 .
63	张嘉晔, 许丽绵. 坤复康胶囊对盆腔炎性疾病后遗症大鼠红细胞免疫功能的影响[J]. 中成药, 2016, 38(10):2253-2256. DOI: 10.3969/j.issn.1001-1528.2016.10.032 .
	ZHANG J Y, XU L M. Effect of kunfukang capsule on erythrocyte immune function in rats with pelvic inflammatory disease sequela[J]. Chin Tradit Pat Med, 2016, 38(10):2253-2256. DOI: 10.3969/j.issn.1001-1528.2016.10.032 .
64	朱平, 李秀玲, 王思磊, 等. 盆炎汤对急性盆腔炎模型大鼠细胞炎症因子、免疫功能及自由基代谢的影响[J]. 中国中医急症, 2019, 28(9):1590-1593, 1618. DOI: 10.3969/j.issn.1004-745X.2019.09.022 .
	ZHU P, LI X L, WANG S L, et al. Effect of penyan decoction on cellular inflammatory factor, immune function and free radical metabolism in rats with acute pelvic inflammation[J]. J Emerg Tradit Chin Med, 2019, 28(9):1590-1593, 1618. DOI: 10.3969/j.issn.1004-745X.2019.09.022 .
65	王春梅, 康燕, 靳紫薇, 等. TLR4抑制剂Tak-242对慢性盆腔炎大鼠炎症指标及病理形态学改变的影响[J]. 中国老年学杂志, 2016, 36(5):1053-1055. DOI: 10.3969/j.issn.1005-9202.2016.05.013 .
	WANG C M, KANG Y, JIN Z W, et al. Effect of TLR4 inhibitor Tak-242 on inflammatory indexes and pathomorphological changes in rats with chronic pelvic inflammatory disease[J]. Chin J Gerontol, 2016, 36(5):1053-1055. DOI: 10.3969/j.issn.1005-9202.2016.05.013 .
66	高升, 高飞, 闫亚楠, 等. 当归芍药散对慢性盆腔炎大鼠外周血Th、Tc细胞和NO、IL-4、IL-10的影响[J]. 中药新药与临床药理, 2016, 27(4):528-533. DOI: 10.19378/j.issn.1003-9783.2016.04.013 .
	GAO S, GAO F, YAN Y N, et al. Effect of Danggui Shaoyao San on Th, Tc cells and nitric oxide, interleukin-4, interleukin-10 levels in peripheral blood of rats with chronic pelvic inflammatory disease[J]. Tradit Chin Drug Res Clin Pharmacol, 2016, 27(4):528-533. DOI: 10.19378/j.issn.1003-9783.2016.04.013 .
67	潘震宇, 李勇敏. 妇炎宁片对细菌性盆腔炎大鼠免疫功能及盆腔炎症的影响[J]. 湖南中医杂志, 2013, 29(10):124-126. DOI: 10.16808/j.cnki.issn1003-7705.2013.10.070 .
	PAN Z Y, LI Y M. Effects of Fuyanning Tablets on immune function and pelvic inflammation in rats with bacterial pelvic inflammatory disease[J]. Hunan J Tradit Chin Med, 2013, 29(10):124-126. DOI: 10.16808/j.cnki.issn1003-7705.2013.10.070 .
68	李冀红, 何延浩, 肖云芳, 等. 康妇炎胶囊对盆腔炎性疾病后遗症模型大鼠免疫功能的影响[J]. 中国妇产科临床杂志, 2017, 18(3):230-232. DOI: 10.13390/j.issn.1672-1861.2017.03.013 .
	LI J H, HE Y H, XIAO Y F, et al. Effect of Kangfuyan capsule on immune function of rats with pelvic inflammatory disease sequelae[J]. Chin J Clin Obstet Gynecol, 2017, 18(3):230-232. DOI: 10.13390/j.issn.1672-1861.2017.03.013 .
69	程乐, 曹耀丹, 陈慧. 丹栀逍遥散对大肠杆菌感染致慢性盆腔炎大鼠模型治疗作用的实验研究[J]. 中国现代中药, 2013, 15(2):93-96. DOI: 10.13313/j.issn.1673-4890.2013.02.014 .
	CHENG L, CAO Y D, CHEN H. The experimental study of Danzhi Xiaoyao power on the therapeutic effect of chronic pelvic inflammatory disease rat model reduced by E.coli infection[J]. Mod Chin Med, 2013, 15(2):93-96. DOI: 10.13313/j.issn.1673-4890.2013.02.014 .
70	汪明德, 叶芳建, 林亚平, 等. 盆宁颗粒对盆腔炎模型鼠血液流变学和镇痛作用的影响[J]. 中华中医药学刊, 2012, 30(8):1719-1722. DOI: 10.13193/j.archtcm.2012.08.25.wangmd.036 .
	WANG M D, YE F J, LIN Y P, et al. Effect of penning granule on hemorheology and analgesia of pelvic inflammatory disease model rats[J]. Chin Arch Tradit Chin Med, 2012, 30(8):1719-1722. DOI: 10.13193/j.archtcm.2012.08.25.wangmd.036 .
71	陈十昔, 高慧, 王楚然, 等. 高氏盆炎方四号方对慢性盆腔炎大鼠抗炎作用及对免疫指标、MCP-1因子影响[J]. 四川中医, 2022, 40(2):45-51.
	CHEN S X, GAO H, WANG C R, et al. Anti-inflammatory effect of Gao's Penyan Formula No.4 on chronic pelvic inflammatory disease rats and its influence on immune index and MCP-1 factor[J]. J Sichuan Tradit Chin Med, 2022, 40(2):45-51.
72	LI X H, LIU Y R, JIANG D H, et al. Research on the mechanism of Chinese herbal medicine Radix Paeoniae Rubra in improving chronic pelvic inflammation disease by regulating PTGS2 in the arachidonic acid pathway[J]. Biomedecine Pharmacother, 2020, 129:110052. DOI: 10.1016/j.biopha.2020.110052 .
73	陈民利, 苗明三. 实验动物学[M]. 北京: 中国中医药出版社, 2020: 99-103.
	CHEN M L, MIAO M S. Laboratory zoology[M]. Beijing: China Press of Traditional Chinese Medicine, 2020: 99-103.
74	邓亚胜, 黄慧, 梁天薇, 等. 基于数据挖掘的慢性支气管炎动物模型应用分析[J]. 中国实验动物学报, 2023, 31(3):327-336. DOI: 10.3969/j.issn.1005-4847.2023.03.007 .
	DENG Y S, HUANG H, LIANG T W, et al. Analysis of the application of animal models of chronic bronchitis by data mining[J]. Acta Lab Animalis Sci Sin, 2023, 31(3):327-336. DOI: 10.3969/j.issn.1005-4847.2023.03.007 .
75	覃倩, 李彤, 尤剑鹏, 等. 盆腔炎鼠类模型构建与评价[J]. 上海交通大学学报(医学版), 2019, 39(10):1218-1222. DOI: 10.3969/j.issn.1674-8115.2019.10.021 .
	QIN Q, LI T, YOU J P, et al. Rat model construction and evaluation of pelvic inflammatory disease[J]. J Shanghai Jiaotong Univ Med Sci, 2019, 39(10):1218-1222. DOI: 10.3969/j.issn.1674-8115.2019.10.021 .
76	李莎莎, 李军. 盆腔炎性疾病动物实验研究进展[J]. 河南中医, 2015, 35(3):606-608. DOI: 10.16367/j.issn.1003-5028.2015.03.0258 .
	LI S S, LI J. Experimental research progress of the animals with pelvic inflammatory diseases[J]. Henan Tradit Chin Med, 2015, 35(3):606-608. DOI: 10.16367/j.issn.1003-5028.2015.03.0258 .
77	YANG M X, LIU S Y, CAI J X, et al. Bile acids ameliorates lipopolysaccharide-induced endometritis in mice by inhibiting NLRP3 inflammasome activation[J]. Life Sci, 2023, 331:122062. DOI: 10.1016/j.lfs.2023.122062 .
78	陈华彪. 左氧氟沙星联合阿奇霉素治疗宫颈炎的临床效果[J]. 临床合理用药, 2024, 17(2):109-112. DOI: 10.15887/j.cnki.13-1389/r.2024.02.032 .
	CHEN H B. Clinical effect of levofloxacin combined with azithromycin in the treatment of cervicitis[J]. Chin J Clin Ration Drug Use, 2024, 17(2):109-112. DOI: 10.15887/j.cnki.13-1389/r.2024.02.032 .
79	吴凡, 王保贵, 胡文, 等. 多西环素联合左氧氟沙星对耐药肺炎克雷伯菌的体内外抗菌活性研究[J]. 中国新药杂志, 2023, 32(23):2425-2433. DOI: 10.3969/j.issn.1003-3734.2023.23.014 .
	WU F, WANG B G, HU W, et al. In vitro and in vivo antibacterial activity of doxycycline combined with levofloxacin against carbapenem-resistant Klebsiella pneumoniae [J]. Chin J N Drugs, 2023, 32(23):2425-2433. DOI: 10.3969/j.issn.1003-3734.2023.23.014 .
80	杨秀伟, 张鹏. 妇科千金方质量标志物研究[J]. 中国现代中药, 2023, 25(5):1105-1112. DOI: 10.13313/j.issn.1673-4890.20220920002 .
	YANG X W, ZHANG P. Quality markers in fuke Qianjin formula[J]. Mod Chin Med, 2023, 25(5):1105-1112. DOI: 10.13313/j.issn.1673-4890.20220920002 .
81	郭颖. 妇炎康治疗慢性盆腔炎的临床疗效[J]. 中国实用医药, 2021, 16(24):169-171. DOI: 10.14163/j.cnki.11-5547/r.2021.24.060 .
	GUO Y. Clinical efficacy of Fuyankang on chronic pelvic inflammatory disease[J]. China Pract Med, 2021, 16(24):169-171. DOI: 10.14163/j.cnki.11-5547/r.2021.24.060 .
82	凌娜, 陈莹. 妇炎宁汤对慢性盆腔炎大鼠模型体内IFN-γ、IL-10的影响[J]. 中华中医药学刊, 2013, 31(5):1142-1144. DOI: 10.13193/j.archtcm.2013.05.184.lingn.068 .
	LING N, CHEN Y. Influence of fuyanning decoction on IFN-γ and IL-10 in rats with chronic pelvic inflammation[J]. Chin Arch Tradit Chin Med, 2013, 31(5):1142-1144. DOI: 10.13193/j.archtcm.2013.05.184.lingn.068 .

造模动物 Modeling animals	频数（百分比/%） Frequency (percentage/%)	体重/g Body weight/g
SD 大鼠 SD rats	155 (71.76)	以（200±20）g为主
Wistar 大鼠 Wistar rats	43 (19.91)	以（200±20）g为主
BALB/c 小鼠 BALB/c mice	8 (3.70)	以（20±1）g为主
新西兰白兔 New Zealand white rabbits	3 (1.39)	文献未提及
C57BL/6 小鼠 C57BL/6 mice	4 (1.85)	以（19±1）g为主
ICR 小鼠 ICR mice	2 (0.93)	以（21±1）g为主
FVB野生型小鼠 FVB wild-type mice	1 (0.46)	文献未提及

造模动物 Modeling animals	频数（百分比/%） Frequency (percentage/%)	体重/g Body weight/g
SD 大鼠 SD rats	155 (71.76)	以（200±20）g为主
Wistar 大鼠 Wistar rats	43 (19.91)	以（200±20）g为主
BALB/c 小鼠 BALB/c mice	8 (3.70)	以（20±1）g为主
新西兰白兔 New Zealand white rabbits	3 (1.39)	文献未提及
C57BL/6 小鼠 C57BL/6 mice	4 (1.85)	以（19±1）g为主
ICR 小鼠 ICR mice	2 (0.93)	以（21±1）g为主
FVB野生型小鼠 FVB wild-type mice	1 (0.46)	文献未提及

造模因素 Modelling factors	造模方法 Modelling methods	频数（百分比/%） Frequency (percentage/%)
化学因素造模 Chemical factor modelling	苯酚胶浆法	59 (27.19)
化学因素造模 Chemical factor modelling	盐酸和脂多糖法	6 (2.77)
物理因素造模 Physical factor modelling	宫腔无菌异物法	4 (1.84)
生物因素造模 Biological factor modelling	单一细菌或微生物法	9 (4.15)
生物因素造模 Biological factor modelling	混合细菌感染法	42 (19.35)
双重因素造模 Dual-factor modelling	机械损伤+混合细菌感染法	80 (36.87)
	苯酚凝胶+机械损伤法	12 (5.53)
	苯酚凝胶+异物法	1 (0.46)
	苯酚凝胶+细菌感染法	1 (0.46)
多因素造模 Multi-factor modelling	饥饿+疲劳干预联合细菌感染法	3 (1.38)

造模因素 Modelling factors	造模方法 Modelling methods	频数（百分比/%） Frequency (percentage/%)
化学因素造模 Chemical factor modelling	苯酚胶浆法	59 (27.19)
化学因素造模 Chemical factor modelling	盐酸和脂多糖法	6 (2.77)
物理因素造模 Physical factor modelling	宫腔无菌异物法	4 (1.84)
生物因素造模 Biological factor modelling	单一细菌或微生物法	9 (4.15)
生物因素造模 Biological factor modelling	混合细菌感染法	42 (19.35)
双重因素造模 Dual-factor modelling	机械损伤+混合细菌感染法	80 (36.87)
	苯酚凝胶+机械损伤法	12 (5.53)
	苯酚凝胶+异物法	1 (0.46)
	苯酚凝胶+细菌感染法	1 (0.46)
多因素造模 Multi-factor modelling	饥饿+疲劳干预联合细菌感染法	3 (1.38)

疾病模型类型 Disease model type	造模周期/d Modelling cycle/d	频数（百分比/%） Frequency (percentage/%)	给药时间/d Administration time/d	频数（百分比/%） Frequency (percentage/%)
急性盆腔炎 Acute pelvic inflammatory disease（APID）	≤7	9 （4.09）	≤7	11 （4.89）
	8~14	10 （4.55）	8~14	4 （1.78）
	15~21	2 （0.91）	15~21	7 （3.11）
	＞28	2 （0.91）	22~28	1 （0.45）
慢性盆腔炎/盆腔炎性疾病后遗症 Chronic pelvic inflammatory disease （CPID）/ sequelae of pelvic inflammatory disease（SPID）	≤7	45 （20.46）	≤7	17 （7.56）
	8~14	47 （21.36）	8~14	45 （20.00）
	15~21	24 （10.45）	15~21	74 （32.89）
	22~28	4 （1.82）	22~28	20 （8.89）
	＞28	27 （12.27）	＞28	10 （4.44）
不明确分期的盆腔炎 Pelvic inflammatory disease with unclear staging	≤7	26 （11.81）	≤7	4 （1.78）
	8~14	10 （4.55）	8~14	10 （4.44）
	15~21	10 （4.55）	15~21	16 （7.11）
	22~28	1 （0.45）	22~28	3 （1.33）
	＞28	4 （1.82）	＞28	3 （1.33）