Effects of Storage Time on Electrolyte Content and pH Value in Rat Serum Samples

doi:10.12300/j.issn.1674-5817.2022.023

Abstract

Abstract:

Objective To observe the effects of serum sample placement time on the detection results of K⁺, Na⁺, Ca²⁺, Cl ^-and pH in sera of SD rats. Methods The sera of 20 male and 20 female SD rats were separated after blood collection from abdominal vein. The samples were covered and stored in a refrigerator at 2-8 ℃. The electrolyte indexes including K⁺, Na⁺, Ca²⁺, Cl^- and pH in serum were measured at 7 time points of 0 h, 2 h, 4 h, 6 h, 24 h, 48 h and 120 h after storage. Results There were no significant differences in the detection results of K⁺ and Na⁺ at each time point (P > 0.05). Compared with the test results at 0 h, the content of Cl^- increased significantly at 48 h and 120 h (P < 0.05); the content of Ca²⁺ decreased significantly at 48 h and 120 hours, and the pH increased significantly at 24 h, 48 h and 120 h (P < 0.05). There was no significant difference in these three indexes within 6 h (P > 0.05). Conclusion The storage time of serum samples of SD rats has a significant impact on the detection results of Ca²⁺, Cl^-and pH in serum. The samples should be covered and stored in a refrigerator at 2-8 ℃ and completed within 6 h after sample preparation.

Key words: Serum, Placement time, K⁺, Na⁺, Ca²⁺, pH, Sprague-Dawley rats

CLC Number:

R-332

Dingshan FENG,Yeyu HUANG,Xiaoxin ZHANG,et al. Effects of Storage Time on Electrolyte Content and pH Value in Rat Serum Samples[J]. Laboratory Animal and Comparative Medicine, 2022, 42(4): 301-305. DOI: 10.12300/j.issn.1674-5817.2022.023.

Figures/Tables 2

References 16

1	樊林花, 李丹, 刘茂林, 等. 清洁级SD大鼠血液生理及生化指标正常参考值的观察[J]. 中国医疗前沿, 2011, 6(19):33-34, 63.
	FAN L H, LI D, LIU M L, et al. Investingation on normal reference range and values of blood physiological and biochemical indexes in clean SD rat[J]. Natl Med Front China, 2011, 6(19):33-34, 63.
2	嘉莉莎. 高血脂对间接离子选择电极法检测电解质的影响[J]. 中国当代医药, 2018, 25(3):148-150. DOI:10.3969/j.issn.1674-4721.2018.03.048 .
	JIA L S. Influence of hyperlipidemia on the detection of electrolyte by indirect ion selective electrode[J]. China Mod Med, 2018, 25(3):148-150. DOI:10.3969/j.issn.1674-4721.2018.03.048 .
3	房玉珠, 姚冬明, 顾红兵, 等. 三种检测系统测定血清电解质的方法学对比与评估[J]. 江苏大学学报(医学版), 2008, 18(1):73-76. DOI:10.13312/j.issn.1671-7783.2008.01.010 .
	FANG Y Z, YAO D M, GU H B, et al. Comparative evaluation of the serum electrolyte determination with three different detection systems[J]. J Jiangsu Univ Med Ed, 2008, 18(1):73-76. DOI:10.13312/j.issn.1671-7783.2008.01.010 .
4	吴芳兰, 应燕萍, 吴卓媚. 中心静脉血与动脉血血浆电解质检测比较的临床研究[J]. 护士进修杂志, 2007, 22(6):494-495. DOI:10.16821/j.cnki.hsjx.2007.06.008 .
	WU F L, YING Y P, WU Z M. Clinical observation on comparing results of plasma electrolyte between blood from central vein and artery[J]. J Nurses Train, 2007, 22(6):494-495. DOI:10.16821/j.cnki.hsjx.2007.06.008 .
5	董树芬. 血样本放置时间和方式对血清电解质测定的影响[J]. 检验医学与临床, 2013, 10(8):1007-1008. DOI:10.3969/j.issn.1672-9455.2013.08.051 .
	DONG S F. Effect of blood sample placement time and method on serum electrolyte determination[J]. Lab Med Clin, 2013, 10(8):1007-1008. DOI:10.3969/j.issn.1672-9455.2013.08.051 .
6	中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准 28天经口毒性试验: [S]. 北京: 中国标准出版社, 2015.
	National Health and Family Planning Commission of the People's Republic of China. National food safety standard, 28 day oral toxicity test: [S]. Beijing: China Standards Press, 2015.
7	陈坚, 黄嘉骅, 黄仲义. 血样处理方法对体内电解质钾、钠、氯测定结果评价的影响研究[J]. 中国药房, 2012, 23(46):4401-4403. DOI:10.6039/j.issn.1001-0408.2012.46.31 .
	CHEN J, HUANG J H, HUANG Z Y. Effect of treatment methods of blood sample on determined results evaluation of K⁺, Na⁺ and Cl^- in vivo [J]. China Pharm, 2012, 23(46):4401-4403. DOI:10.6039/j.issn.1001-0408.2012.46.31 .
8	王晓刚, 农乐关, 刘旭, 等. 不同类型外周血标本电解质检测结果比较[J]. 国际检验医学杂志, 2015, 36(10):1356-1357. DOI:10.3969/j.issn.1673-4130.2015.10.015 .
	WANG X G, NONG L G, LIU X, et al. Comparison of electrolyte levels between different peripheral blood samples[J]. Int J Lab Med, 2015, 36(10):1356-1357. DOI:10.3969/j.issn.1673-4130.2015.10.015 .
9	李情操, 穆银玉, 卢雯君, 等. 不同真空采血管和标本放置时间对电解质检测结果的影响[J]. 上海预防医学, 2014, 26(3):149-151. DOI:10.19428/j.cnki.sjpm.2014.03.016 .
	LI Q C, MU Y Y, LU W J, et al. Effect of different placed time for vacutainer and specimen on electrolyte test results[J]. Shanghai J Prev Med, 2014, 26(3):149-151. DOI:10.19428/j.cnki.sjpm.2014.03.016 .
10	吴琰. 不同存储条件对血样电解质及凝血功能指标检测结果的影响[J]. 医疗装备, 2021, 34(9):57-59. DOI:10.3969/j.issn.1002-2376.2021.09.026 .
	WU Y. Influences of different storage conditions on the detection results of blood electrolyte and coagulation function[J]. Med Equip, 2021, 34(9):57-59. DOI:10.3969/j.issn.1002-2376.2021.09.026 .
11	杨利红, 彭小燕, 刘鹏. 样品再定位对电解质分析仪检测结果的影响[J]. 中国城乡企业卫生, 2015, 30(2):115-116. DOI:10.16286/j.1003-5052.2015.02.050 .
	YANG L H, PENG X Y, LIU P. Influence of sample repositioning on the detection results of electrolyte analyzer[J]. Chin J Urban Rural Enterp Hyg, 2015, 30(2):115-116. DOI:10.16286/j.1003-5052.2015.02.050 .
12	钟昆华. 血清电解质钾、钠、氯检测方法比较[J]. 四川解剖学杂志, 2014, 22(4):12-14. DOI:10.3969/j.issn.1005-1457.2014.070 .
	ZHONG K H. Comparison on methods analysis of serum electrolyte of K, Na and Cl[J]. Sichuan J Anat, 2014, 22(4):12-14. DOI:10.3969/j.issn.1005-1457.2014.070 .
13	张耀东. 血清K⁺、Na⁺、Cl^-、HCO₃ ^-检测结果与放置时间相关性的研究[J]. 中外医疗, 2011, 30(3):38, 40. DOI:10.16662/j.cnki.1674-0742.2011.03.050 .
	ZHANG Y D. Study on the correlation between the detection results of serum K⁺, Na⁺, Cl^- and HCO₃ ^- and storage time[J]. China Foreign Med Treat, 2011, 30(3):38, 40. DOI:10.16662/j.cnki.1674-0742.2011.03.050 .
14	晏文强, 邓涛, 刘杨, 等. 血液标本不同保存时间及条件对生化检测结果的影响[J]. 湖北医药学院学报, 2017, 36(4):334-337. DOI:10.13819/j.issn.1006-9674.2017.04.011 .
	YAN W Q, DENG T, LIU Y, et al. The effect of preservation time and condition of blood samples on biochemical tests[J]. J Hubei Univ Med, 2017, 36(4):334-337. DOI:10.13819/j.issn.1006-9674.2017.04.011 .
15	杨惠元. 3种真空采血管不同时间检测电解质的结果比较[J]. 国际检验医学杂志, 2015, 36(19):2893-2894. DOI:10.3969/j.issn.1673-4130.2015.19.053 .
	YANG H Y. Comparison of electrolyte detection results of three kinds of vacuum blood collection tubes at different time[J]. Int J Lab Med, 2015, 36(19):2893-2894. DOI:10.3969/j.issn.1673-4130.2015.19.053 .
16	李红, 敬海明, 林长缨, 等. 大鼠血清葡萄糖测定分析的影响因素[J]. 实验动物与比较医学, 2021, 41(5): 466-468. DOI:10.12300/j.issn.1674-5817.2020.097 .
	LI H, JING H M, LIN C Y, et al. Influence factors of serum glucose measurement and analysis in rats[J]. Lab Animal Comp Med, 2021, 41(5):466-468. DOI:10.12300/j.issn.1674-5817.2020.097 .

实验时间 Test time	大鼠性别 Rat sex
实验时间 Test time	雄性Male (n = 20)	雌性Female (n = 20)
起始 Initial	95.7±3.5	92.0±4.0
第1周 1^st week	169.0±6.9	152.0±7.1
第2周 2^nd week	246.5±7.4	197.7±13.8
第3周 3^rd week	313.8±12.6	228.1±16.4
第4周 4^th week	350.5±15.0	244.1±18.5

实验时间 Test time	大鼠性别 Rat sex
实验时间 Test time	雄性Male (n = 20)	雌性Female (n = 20)
起始 Initial	95.7±3.5	92.0±4.0
第1周 1^st week	169.0±6.9	152.0±7.1
第2周 2^nd week	246.5±7.4	197.7±13.8
第3周 3^rd week	313.8±12.6	228.1±16.4
第4周 4^th week	350.5±15.0	244.1±18.5

性别 Rat sex	放置时间/h Placement time /h	K⁺ c/(mmol·L^-1)	Na⁺ c/(mmol·L^-1)	Cl^- c/(mmol·L^-1)	Ca²⁺ c/(mmol·L^-1)	pH
雄性Male (n = 20)	0	5.27±1.97	142.96±1.09	99.21±1.14	2.24±0.13	7.53±0.11
	2	5.33±2.22	143.43±1.35	99.81±1.06	2.28±0.07	7.44±0.07
	4	5.31±2.25	142.93±1.08	99.33±1.37	2.31±0.08	7.49±0.10
	6	5.35±2.25	142.59±1.22	99.75±1.07	2.32±0.08	7.54±0.08
	24	5.37±2.23	143.43±1.39	99.42±1.34	2.23±0.16	7.65±0.07*
	48	5.35±2.21	142.57±0.94	100.30±1.47*	2.02±0.20*	7.68±0.08*
	120	5.34±2.16	142.62±1.23	100.19±1.57*	1.87±0.16*	7.72±0.07*
雌性Female (n = 19)	0	5.26±1.36	143.34±0.92	99.66±1.59	2.10±0.18	7.61±0.08
	2	5.23±1.47	144.29±1.26	99.50±1.58	2.29±0.06	7.26±0.10
	4	5.38±1.43	143.24±1.09	100.12±1.61	2.34±0.07	7.44±0.09
	6	5.19±1.41	142.98±1.14	100.19±2.04	2.32±0.08	7.50±0.09
	24	5.29±1.38	143.88±1.01	99.37±1.84	2.03±0.20	7.66±0.08*
	48	5.33±1.43	142.95±0.92	101.00±1.89*	1.86±0.15*	7.69±0.08*
	120	5.28±1.39	143.62±1.55	102.23±2.23*	1.71±0.09*	7.76±0.08*

性别 Rat sex	放置时间/h Placement time /h	K⁺ c/(mmol·L^-1)	Na⁺ c/(mmol·L^-1)	Cl^- c/(mmol·L^-1)	Ca²⁺ c/(mmol·L^-1)	pH
雄性Male (n = 20)	0	5.27±1.97	142.96±1.09	99.21±1.14	2.24±0.13	7.53±0.11
	2	5.33±2.22	143.43±1.35	99.81±1.06	2.28±0.07	7.44±0.07
	4	5.31±2.25	142.93±1.08	99.33±1.37	2.31±0.08	7.49±0.10
	6	5.35±2.25	142.59±1.22	99.75±1.07	2.32±0.08	7.54±0.08
	24	5.37±2.23	143.43±1.39	99.42±1.34	2.23±0.16	7.65±0.07*
	48	5.35±2.21	142.57±0.94	100.30±1.47*	2.02±0.20*	7.68±0.08*
	120	5.34±2.16	142.62±1.23	100.19±1.57*	1.87±0.16*	7.72±0.07*
雌性Female (n = 19)	0	5.26±1.36	143.34±0.92	99.66±1.59	2.10±0.18	7.61±0.08
	2	5.23±1.47	144.29±1.26	99.50±1.58	2.29±0.06	7.26±0.10
	4	5.38±1.43	143.24±1.09	100.12±1.61	2.34±0.07	7.44±0.09
	6	5.19±1.41	142.98±1.14	100.19±2.04	2.32±0.08	7.50±0.09
	24	5.29±1.38	143.88±1.01	99.37±1.84	2.03±0.20	7.66±0.08*
	48	5.33±1.43	142.95±0.92	101.00±1.89*	1.86±0.15*	7.69±0.08*
	120	5.28±1.39	143.62±1.55	102.23±2.23*	1.71±0.09*	7.76±0.08*

[1]	GONG Leilei, WANG Xiaoxia, FENG Xuewei, LI Xinlei, ZHAO Han, ZHANG Xueyan, FENG Xin. A Mouse Model and Mechanism Study of Premature Ovarian Insufficiency Induced by Different Concentrations of Cyclophosphamide [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 403-410.
[2]	QIN Chao, LI Shuangxing, ZHAO Tingting, JIANG Chenchen, ZHAO Jing, YANG Yanwei, LIN Zhi, WANG Sanlong, WEN Hairuo. Study on the 90-day Feeding Experimental Background Data of SD Rats for Drug Safety Evaluation [J]. Laboratory Animal and Comparative Medicine, 2025, 45(4): 439-448.
[3]	PAN Yicong, JIANG Wenhong, HU Ming, QIN Xiao. Optimization of Surgical Procedure and Efficacy Evaluation of Aortic Calcification Model in Rats with Chronic Kidney Disease [J]. Laboratory Animal and Comparative Medicine, 2025, 45(3): 279-289.
[4]	WANG Chenjuan, YANG Lingyan, WANG Lipeng, SUN Xueping, LI Jingwen, GUO Lianxiang, RONG Rong, SHI Changjun. Diagnosis of an Outbreak of Canine Distemper in Cynomolgus Monkeys in an Experimental Monkey Farm in 2019 [J]. Laboratory Animal and Comparative Medicine, 2025, 45(3): 360-367.
[5]	TONG Xiyang, QUE Changtian, ZHANG Feng, ZHAO Lu, WANG Hongping. Analysis of Common Causes of Out-of-Specification Results in the Test for Depressor Substances [J]. Laboratory Animal and Comparative Medicine, 2025, 45(3): 331-339.
[6]	LUO Lianlian, YUAN Yanchun, WANG Junling, SHI Guangsen. Advances in Mouse Models of Amyotrophic Lateral Sclerosis [J]. Laboratory Animal and Comparative Medicine, 2025, 45(3): 290-299.
[7]	JIANG Meng, HAO Shulan, TONG Liguo, ZHONG Qiming, GAO Zhenfei, WANG Yonghui, WANG Xixing, JI Haijie. Dynamic Evaluation of Vinorelbine-Induced Phlebitis of Dorsalis Pedis Vein in a Rat Model [J]. Laboratory Animal and Comparative Medicine, 2025, 45(3): 251-258.
[8]	KONG Zhihao, WEI Xiaofeng, YU Lingzhi, FENG Liping, ZHU Qi, SHI Guojun, WANG Chen. Isolation and Identification of Staphylococcus xylosus in Nude Mice with Squamous Skin Scurfs [J]. Laboratory Animal and Comparative Medicine, 2025, 45(3): 368-375.
[9]	WANG Biying, LU Jiashuo, ZAN Guiying, CHEN Ruosong, CHAI Jingrui, LIU Jinggen, WANG Yujun. Establishment Methods and Application Progress of Rodent Models for Drug Addiction [J]. Laboratory Animal and Comparative Medicine, 2025, 45(2): 158-166.
[10]	TANG Yingen, FENG Yaxian, ZHONG Min, WEI Zhen, WANG Lie, LIU Diwen. Polymorphism and Tissue Expression Analysis of TYR and MC1R Genes in Guinea Pigs with Different Coat-Color Phenotypes [J]. Laboratory Animal and Comparative Medicine, 2025, 45(1): 21-29.
[11]	TIAN Fang, PAN Bin, SHI Jiayi, XU Yanyi, LI Weihua. Advances in Development of PM_2.5-Exposed Animal Models and Their Application in Reproductive Toxicity Research [J]. Laboratory Animal and Comparative Medicine, 2024, 44(6): 626-635.
[12]	LIU Siyu, LAI Yuezhao, GUO Wenting, CHEN Xuejin. Advances in Nucleic Acid Drugs and Gene Therapies based on Animal Models of Duchenne Muscular Dystrophy [J]. Laboratory Animal and Comparative Medicine, 2024, 44(6): 613-625.
[13]	LIN Qingqing, DAI Jinlong, CHEN Zhisen, GUO Jianmin, YANG Wei. Pathological Diagnosis of Systemic Amyloidosis in a New Zealand White Rabbit [J]. Laboratory Animal and Comparative Medicine, 2024, 44(6): 695-699.
[14]	YANG Jin, YU Shiya, LIN Nan, FANG Yongchao, ZHAO Hu, QIU Jinwei, LIN Hongming, CHEN Huiyan, WANG Yu, WU Weihang. Effect of Modified Duodenal Exclusion Surgery on Glucose Metabolism in Rats with Type 2 Diabetes Mellitus [J]. Laboratory Animal and Comparative Medicine, 2024, 44(5): 523-530.
[15]	QI Longju, CHEN Shiyuan, LIAO Zehua, SHI Yuanhu, SUN Yuyu, WANG Qinghua. Transcriptomic Analysis of Menstrual Blood-Derived Stem Cells Transplantation Combined with Exercise Training in Promoting Spinal Cord Injury Recovery in Rats [J]. Laboratory Animal and Comparative Medicine, 2024, 44(5): 531-542.