Effects of Storage Time on Electrolyte Content and pH Value in Rat Serum Samples

doi:10.12300/j.issn.1674-5817.2022.023

Abstract

Abstract:

Objective To observe the effects of serum sample placement time on the detection results of K⁺, Na⁺, Ca²⁺, Cl ^-and pH in sera of SD rats. Methods The sera of 20 male and 20 female SD rats were separated after blood collection from abdominal vein. The samples were covered and stored in a refrigerator at 2-8 ℃. The electrolyte indexes including K⁺, Na⁺, Ca²⁺, Cl^- and pH in serum were measured at 7 time points of 0 h, 2 h, 4 h, 6 h, 24 h, 48 h and 120 h after storage. Results There were no significant differences in the detection results of K⁺ and Na⁺ at each time point (P > 0.05). Compared with the test results at 0 h, the content of Cl^- increased significantly at 48 h and 120 h (P < 0.05); the content of Ca²⁺ decreased significantly at 48 h and 120 hours, and the pH increased significantly at 24 h, 48 h and 120 h (P < 0.05). There was no significant difference in these three indexes within 6 h (P > 0.05). Conclusion The storage time of serum samples of SD rats has a significant impact on the detection results of Ca²⁺, Cl^-and pH in serum. The samples should be covered and stored in a refrigerator at 2-8 ℃ and completed within 6 h after sample preparation.

Key words: Serum, Placement time, K⁺, Na⁺, Ca²⁺, pH, Sprague-Dawley rats

CLC Number:

R-332

Dingshan FENG,Yeyu HUANG,Xiaoxin ZHANG,et al. Effects of Storage Time on Electrolyte Content and pH Value in Rat Serum Samples[J]. Laboratory Animal and Comparative Medicine, 2022, 42(4): 301-305. DOI: 10.12300/j.issn.1674-5817.2022.023.

Figures/Tables 2

References 16

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实验时间 Test time	大鼠性别 Rat sex
实验时间 Test time	雄性Male (n = 20)	雌性Female (n = 20)
起始 Initial	95.7±3.5	92.0±4.0
第1周 1^st week	169.0±6.9	152.0±7.1
第2周 2^nd week	246.5±7.4	197.7±13.8
第3周 3^rd week	313.8±12.6	228.1±16.4
第4周 4^th week	350.5±15.0	244.1±18.5

实验时间 Test time	大鼠性别 Rat sex
实验时间 Test time	雄性Male (n = 20)	雌性Female (n = 20)
起始 Initial	95.7±3.5	92.0±4.0
第1周 1^st week	169.0±6.9	152.0±7.1
第2周 2^nd week	246.5±7.4	197.7±13.8
第3周 3^rd week	313.8±12.6	228.1±16.4
第4周 4^th week	350.5±15.0	244.1±18.5

性别 Rat sex	放置时间/h Placement time /h	K⁺ c/(mmol·L^-1)	Na⁺ c/(mmol·L^-1)	Cl^- c/(mmol·L^-1)	Ca²⁺ c/(mmol·L^-1)	pH
雄性Male (n = 20)	0	5.27±1.97	142.96±1.09	99.21±1.14	2.24±0.13	7.53±0.11
	2	5.33±2.22	143.43±1.35	99.81±1.06	2.28±0.07	7.44±0.07
	4	5.31±2.25	142.93±1.08	99.33±1.37	2.31±0.08	7.49±0.10
	6	5.35±2.25	142.59±1.22	99.75±1.07	2.32±0.08	7.54±0.08
	24	5.37±2.23	143.43±1.39	99.42±1.34	2.23±0.16	7.65±0.07*
	48	5.35±2.21	142.57±0.94	100.30±1.47*	2.02±0.20*	7.68±0.08*
	120	5.34±2.16	142.62±1.23	100.19±1.57*	1.87±0.16*	7.72±0.07*
雌性Female (n = 19)	0	5.26±1.36	143.34±0.92	99.66±1.59	2.10±0.18	7.61±0.08
	2	5.23±1.47	144.29±1.26	99.50±1.58	2.29±0.06	7.26±0.10
	4	5.38±1.43	143.24±1.09	100.12±1.61	2.34±0.07	7.44±0.09
	6	5.19±1.41	142.98±1.14	100.19±2.04	2.32±0.08	7.50±0.09
	24	5.29±1.38	143.88±1.01	99.37±1.84	2.03±0.20	7.66±0.08*
	48	5.33±1.43	142.95±0.92	101.00±1.89*	1.86±0.15*	7.69±0.08*
	120	5.28±1.39	143.62±1.55	102.23±2.23*	1.71±0.09*	7.76±0.08*

性别 Rat sex	放置时间/h Placement time /h	K⁺ c/(mmol·L^-1)	Na⁺ c/(mmol·L^-1)	Cl^- c/(mmol·L^-1)	Ca²⁺ c/(mmol·L^-1)	pH
雄性Male (n = 20)	0	5.27±1.97	142.96±1.09	99.21±1.14	2.24±0.13	7.53±0.11
	2	5.33±2.22	143.43±1.35	99.81±1.06	2.28±0.07	7.44±0.07
	4	5.31±2.25	142.93±1.08	99.33±1.37	2.31±0.08	7.49±0.10
	6	5.35±2.25	142.59±1.22	99.75±1.07	2.32±0.08	7.54±0.08
	24	5.37±2.23	143.43±1.39	99.42±1.34	2.23±0.16	7.65±0.07*
	48	5.35±2.21	142.57±0.94	100.30±1.47*	2.02±0.20*	7.68±0.08*
	120	5.34±2.16	142.62±1.23	100.19±1.57*	1.87±0.16*	7.72±0.07*
雌性Female (n = 19)	0	5.26±1.36	143.34±0.92	99.66±1.59	2.10±0.18	7.61±0.08
	2	5.23±1.47	144.29±1.26	99.50±1.58	2.29±0.06	7.26±0.10
	4	5.38±1.43	143.24±1.09	100.12±1.61	2.34±0.07	7.44±0.09
	6	5.19±1.41	142.98±1.14	100.19±2.04	2.32±0.08	7.50±0.09
	24	5.29±1.38	143.88±1.01	99.37±1.84	2.03±0.20	7.66±0.08*
	48	5.33±1.43	142.95±0.92	101.00±1.89*	1.86±0.15*	7.69±0.08*
	120	5.28±1.39	143.62±1.55	102.23±2.23*	1.71±0.09*	7.76±0.08*

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