Population Genetic Quality Analysis of Microsatellite DNA in Wistar Rats Based on T/CALAS 21—2017

doi:10.12300/j.issn.1674-5817.2021.073

Abstract

Abstract: Objective To analyze the genetic quality of an outbred stock of Wistar rats in different periods by T/CALAS 21—2017 method, and to evaluate the applicability of the association standard. Methods Wistar rats selected from the same outbred stock in 2015 and 2019 were named as group A and group B, respectively. Twenty-five pairs of microsatellite primers were used to get genetic parameters for quality analysis of the outbred stock Wistar rats according to the association standard T/CALAS 21—2017. Polymorphism information content (PIC) was used to analyze the polymorphism of the loci. Results One hundred alleles in group A and 69 alleles in group B were obtained. The average heterozygosity of the two groups was 0.574 and 0.447, while the average PIC was 0.541 and 0.393, respectively. Conclusion The association standard T/CALAS 21—2017 has good applicability in the genetic quality analysis of outbred stock rats, and the genetic diversity of group A is better than that of group B.

Key words: Wistar rats, T/CALAS 21—2017, Outbred stock, Microsatellite DNA, Genetic diversity

CLC Number:

Q95-33
R-332

WEI Jie, ZUO Qin, WANG Hong, LI Huan, ZHOU Jiaqi, GUANG Jiaona, FAN Tao, LIU Zuomin, FU Rui, YUE Bingfei. Population Genetic Quality Analysis of Microsatellite DNA in Wistar Rats Based on T/CALAS 21—2017[J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 528-534.

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