Establishment of Otitis Media with Effusion Induced by Lipopolysaccharides in Rat

doi:10.3969/j.issn.1674-5817.2011.06.002

Abstract

Abstract: Objective To establishing the rat model of otitis media with effusion (OME). Methods The rats were inoculated with lipopolysaccharides (LPS) into the middle ear cavity through tympanic membrane. The pathologic changes of middle ear cavity and mucocilliary transporting system on the tympanic ostium eustachian tube were observed by light microscope and scan electrical microscope on days 1, 3, 7 after injecting LPS, respectively. Results Effusion were observed on day 1 after injecting LPS through tympanic membrane and disappeared after 7 days through otoscope.The pathologic changes were observed most severe on day 1 and returned to normal at 7 days under light microscope, and the impairment of mucociliary transport system such as lodging, shedding of cilias were observed on day 1 after injecting LPS and returned to normal after 7 days with scan electrical microscope. Conclusions Lipopolysaccharides (LPS) inoculated into the middle ear cavity through tympanic membrane can induce the inflammatory response and effusion of the middle ear, which can effectively establish the rat model of otitis media with effusion.

Key words: Otitis media with effusion, Models, Rat, Lipopolysaccharide

CLC Number:

Q95-33

LI Xiang-cui, ZHANG Yi, LI Ji-ping, WANG Jia-dong. Establishment of Otitis Media with Effusion Induced by Lipopolysaccharides in Rat[J]. Laboratory Animal and Comparative Medicine, 2011, 31(6): 410-413.

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