Effect of Implantable Vagus Nerve Stimulator on Epilepsy Models Induced by Kainic Acid in Rabbit

doi:10.3969/j.issn.1674-5817.2015.06.007

Abstract

Abstract: Objectives To study the intervention effect of implantable vagus nerve-stimulator (VNS) on epileptic rabbit model induced by kainic acid (KA). Methods The epileptic model of adult rabbit was established through the micro-injection of kainic acid into the cerebral cortex of animal. To relieve the epilepsy by VNS, the spiral electrode was placed on the vagus nerve, while the stimulator was implanted in subcutaneous tissue of the left animal back. Then 18 rabbits were randomly divided into 3 groups including epilepsy model group (group A, n=6), VNS pretreatment group (group B, n=6) and the VNS treatment group (group C, n=6). In group A, none VNS was implanted in the epileptic animal. In group B and C, VNSs were implanted 7 days before the KA injection for the epilepsy induction. The electricity stimulation was performed 2 hours before epilepsy in group B. However, stimulation carried out immediately when epilepsy emerges in group C. Epileptic behaviors and electroencephalography were observed and tested subsequently in all groups. The subcutaneous tissues around VNS were also observed via H.E stain to show the biocompatibility of VNS. Results All the animals showed stable epileptic seizure induced by KA and survived after the VNS plantation without obvious anomaly. The epileptiform electrocorticogram were ameliorated and the epileptic seizure remarkably suppressed in group B and were slightly restrained in group C. The tissues around the implanted VNS showed no obvious distinctions comparing with normal animal tissues in H.E stain. Conclusion VNS could alleviate the seizure of epileptic animal and show better effect when the vagus nerve is stimulated ahead of time. The implantable VNS is also biocompatible with animal subcutaneous tissues.

Key words: Vagus nerve-stimulator(VNS), Rabbit, Epileptic model

CLC Number:

Q95-33

YIN Yin, ZHOU Hui-ying, XIAO Chun-lan, ZHANG Xin-guo, ZHOU Zheng-yu, WANG Jing. Effect of Implantable Vagus Nerve Stimulator on Epilepsy Models Induced by Kainic Acid in Rabbit[J]. Laboratory Animal and Comparative Medicine, 2015, 35(6): 462-466.

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