Laboratory Animal and Comparative Medicine ›› 2014, Vol. 34 ›› Issue (2): 126-130.DOI: 10.3969/j.issn.1674-5817.2014.02.009

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Effects of High Fat Diet on Arterial Ring's Tension in SD Rats

ZHANG Yi1, ZHAN Wei-wei1, WU Yong-ji2, ZHOU Wu-gang2, CHEN Dong-rui2, JIANG Wei-min3, ZHENG Lin3   

  1. 1. Department of Ultrasound, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;
    2. Shanghai Institute of Hypertention,Shanghai 200025, China;
    3. Department of Pathology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China
  • Received:2013-09-09 Online:2014-04-25 Published:2014-04-25

Abstract: Objective To observe the changes of arterial ring's tension in SD rats with hypercholesterolemia and atherosclerosis. Methods In vitro isometric tention experiment was used to measure arterial ring's maximum contractile tension, arterial ring'maximum relaxation pencentage of the aorta in cholesterol-fed SD Rats (group T1, n=10, for 4 weeks; group T2, n=10, for 12 weeks) and normal control rats(group C1, n=10; group C2, n =10). All parameters and blood biochemical markers(total cholesterol, triglycerides, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol) among groups were analyzed with ANOVE factor analysis.Correlation was analyzed with Pearson analysis.Light microscopic evaluation was used to demonstrate atherosclerotic changes in the aorta. Result The maximum contractile tension value to KCl and phenephrine and the maximum relaxation pencentage of the aorta in group T1 were significantly lower than those of group C1 controls (P<0.05). The maximum relaxation pencentage of the aorta in group T2 rats were significantly lower than those of group C2 controls (P<0.05). The maximum contractile tension value to KCl and phenephrine in group T2 were significantly higher than those of group T1(P=0.001, P<0.05). However, no significances were found with the maximum relaxation percentage in group T1 and T2 (P>0.05). Correlation analysis shows: the maximum contractile tension value of the aorta was positively correlated with triglycerides (r=0.435, P<0.05). The maximum relaxation pencentage of the aorta was negatively correlated with systolic pressure, total cholesterol and low-density lipoprotein cholesterol (r=-0.318, P=0.046, r=-0.449, P=0.004; r=-0.411, P=0.008), also it was negatively correlated with high-density lipoprotein cholesterol(r=0.324, P=0.041). Light microscopy confirmed morphologic typical changes of aortic atherosclerosis in group T1 and T2. Conclution High fat diet will lower the ability of rats's arterial contraction and relaxation in earlier state. The maximum relaxation percentage was much lower associated with the increasement of total cholesterol and low density lipoprotein.

Key words: Hypercholesterolemia, Arterial ring's tension;, Atherosclerosis, SD rats

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