Mechanism of Circulating Endothelial MicroRNA Related to Atherosclerosis Induced by Inflammatory Response of Macrophages in Mouse

doi:10.3969/j.issn.1674-5817.2019.03.005

Abstract

Abstract: Objective To study the mechanism of microRNA related to circulating endothelial particulates (EMPs) on atherosclerosis (AS) induced by inflammatory response of macrophages. Methods There were 206 male ApoE^-/- mice of SPF grade and 50 control groups. The experimental group was randomly divided into three groups: AS model (AS) group, NC-miRNA group and miRNA-19b inhibitor group with 52 in each group, using oil red staining of atherosclerotic lesions in the histology were observed, calculated plaque area ratio, and detect the serum cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) biochemical index expression level of TNF alpha ELISA to detect vascular inflammation factors, IL-1, the change of IL-6 and IL-10, bcl-2, cleaved-PARP and Bax expression were detected by Western blot, mir-19b expression was detected by real-time quantitative PCR, and the apoptosis rate of macrophages was detected by flow cytometry. Results Micrornas-19b inhibitor group can decrease due to the increase in the thickness of the blood vessels of AS, reduce atherosclerotic plaque area percentage, the blood lipid level of TC, TG and LDL-C in the lower, HDL-C level has increased, promoting apoptosis related proteins Bax, cleaved - PARP expression decreased, and resistance to apoptosis related proteins increased the expression of Bcl-2 and proinflammatory factor in vascular IL-1, IL-6 and TNF-〈 level will be significantly lower, anti-inflammatory factor of IL-10 level is higher, At the same time, the apoptosis rate of endovascular macrophages decreased. Conclusion The presence of circulating endothelial microcore-related mirna-19b can reduce the apoptosis of macrophages, and promote the development of peripheral inflammation by up-regulating pro-inflammatory factors, thus promoting the development of AS.

Key words: Cyclic endothelial particles(EMPs), Micrornas-19b, Macrophages, Inflammat-ory factors, Atherosclerosisi (AS)

CLC Number:

R562.2+1Q95-33

SUN Li-hua, ZHANG Ying, CAO Gui-qiu, LI Peng, HU Qiang, ZHANG Ya-ling, XING Shi-feng. Mechanism of Circulating Endothelial MicroRNA Related to Atherosclerosis Induced by Inflammatory Response of Macrophages in Mouse[J]. Laboratory Animal and Comparative Medicine, 2019, 39(3): 201-207.

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