Intragastric Estrogen Administration Induced Guinea Pig Model of Uterine Leiomyoma

doi:10.3969/j.issn.1674-5817.2016.03.007

Abstract

Abstract: Objective To establish the guinea pig model of uterine leiomyoma by intragastric administration of estrogen and to discuss the effect of different cycles of administration on formation of uterine leiomyoma. Method Mature female guinea pigs were divided into short term group and long term group, and intragastric administrated with estradiol valerate at 0.1 mg/100 g body weight twice a week for 6 weeks and 8 weeks respectively. The control was intragastric administrated with normal saline with same volume. After 6 or 8 weeks, the guinea pigs were sacrificed. The serum levels of estrogen and progesterone were measured by ELISA and the leiomyoma forming rate was measured. Histological changes were compared between treatment group and control group with HE staining. Results The leiomyoma forming rate in short term group was 30% and long term group was 40%. The average total rate was 35%. The serum level of estrogen in model animals was increased significantly and the progesterone decreased in long term group. Histopathological examination confirmed that the guinea pig model of uterine leiomyoma were established successfully. Conclusions Intragastric estrogen administration was a simple and effective method for establishing uterine leiomyoma guinea pigs model.

Key words: Uterine leiomyoma, Guinea pigs, Estrogen, Intragastric administration

CLC Number:

Q95-33

ZHANG Yue-han, WEN Jing, ZHU Hai-tao, ZHAO Guang-zong, LI Xiao-yu. Intragastric Estrogen Administration Induced Guinea Pig Model of Uterine Leiomyoma[J]. Laboratory Animal and Comparative Medicine, 2016, 36(3): 195-199.

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