Remifentanil Combined with Cord Blood Mesenchymal Stem Cells Transplantation on Hindlimb Function and Electrophysiology in Spinal Cord Injured Rats

doi:10.3969/j.issn.1674-5817.2017.05.005

Abstract

Abstract: Objective To determine the effects of cord blood mesenchymal stem cells (UCMSC) transplantation combined with remifentanil on electrophysiology and hindlimb functionon in spinal cord injured rats. Methods The spinal cord injury model was established in 83 adult Wistar rats, the model rats were randomly divided into four groups. ①UCMSC transplantation group, via the tail vein infusion of equal volumes UCMSC cell sap. ② control group, refers to tail vein injection medium group. ③ remifentanil group, remifentanil injection (2 mL·kg·h^-1) via tail vein infusion of 4 h. ④ combined group, UCMSC cells injected intravenously via the tail vein and combined with infusion of remifentanil injection solution (2 ml·kg·h^-1) continued 4 h. The spinal cord injury (BBB) score was evaluated before and 1 week, 2 weeks, 4 weeks and 6 weeks after transplantation. The modified Tarlov score and the slant plate test were used to evaluate the motor function. The survival and pathology of PKH-26-labeled UCMSC were observed by fluorescence microscopy at 4 weeks after transplantation. HRP (Horse Reddish Peroxidase) was used to analyze the regeneration of nerve fibers in the fourth week. MEP motor evoked potential and SEP somatosensory evoked potential were used to analyze the nerve Physiological recovery. Results Compared with UCMSC transplantation group and remifentanil group, UCMSC transplantation group and remifentanil group were better than the control group. There was a small amount of axonal-like structure in the injury area of Rufentini group and UCMSC transplantation group. The syndromes of the syringes were relatively small, and the ganglion-like structure was seen in the combined group. No syringomyelia was found. HE staining, the control group can be seen spinal cord loss and syringomyelia formation, no nerve axon through. The number of HRP-positive nerve fibers and PKH-26 positive cells were the lowest in the control group and the most in the control group, UCMSC transplantation group and remifentanil group were significantly different between the two groups at 4 weeks after transplantation (P<0.05). MEP incubation group> remifentanil group and UCMSC transplantation group> and the difference between the groups were statistically significant (P<0.05); SEP incubation period, control group, remifentanil group and UCMSC group, and the difference was statistically significant (P<0.05). Conclusion Remifentanil may play a role in neuroprotective effects on spinal cord injury. At the same time, UCMSC transplanted with remifentanil can promote the regeneration of neuronal synapses in rats with spinal cord injury and improve the electrophysiological function and limb motor function of rats.

Key words: Cord Blood Mesenchymal Stem Cells (UCMSC), Remifentanil, Rat, Spinal cord injury

CLC Number:

Q95-33

LI Min, YANG Lei, YE Kui. Remifentanil Combined with Cord Blood Mesenchymal Stem Cells Transplantation on Hindlimb Function and Electrophysiology in Spinal Cord Injured Rats[J]. Laboratory Animal and Comparative Medicine, 2017, 37(5): 363-371.

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