Construction of Afp-cre-lacZ Transgenic Mouse Model

doi:10.3969/j.issn.1674-5817.2017.02.002

Abstract

Abstract: Objective To study the function of alpha-fetoprotein (Afp) positive cells during tissues growth and repair or in tumorigenesis, Afp-cre-lacZ linage tracing mouse model was established. Methods Afp-CreERT mice were established by microinjection and identified by real time PCR. Afp-CreERT mice were crossed with Rosa26-lacZ mice. Cre/lacZ both positive mice were kept as Afp-cre-lacZ mice. Results Fifty-six transgenic mice were born after microinjection. Four of them genotyped Cre positive were kept as founder. Each founder was established a strain. Cre expression of each strain was identified via real time PCR. Thus Afp-CreERT transgenic mouse was established. The Cre/lacZ both positive offspring of Afp-CreERT mice were crossed with Rosa26-lacZ mice were kept as Afp-cre-lacZ mice. Identified by real time PCR, X-gal staining and immunofluorescence, Afp-cre-lacZ mice were observed that they could be used in lineage tracing and further studies. Conclusions The Afp-cre-lacZ mice established may be used as a tool for lineage tracing studies.

Key words: Alpha-fetoprotein (Afp), Transgenic mouse model, Lineage tracing

CLC Number:

Q95-33

GU Xiao-wen,SUN Rei-lin,FEI Jian. Construction of Afp-cre-lacZ Transgenic Mouse Model[J]. Laboratory Animal and Comparative Medicine, 2017, 37(2): 89-93. DOI: 10.3969/j.issn.1674-5817.2017.02.002.

References

[1] Sun W, Liu Y, Shou D, et al.AFP (alpha fetoprotein): who are you in gastrology?[J]. Cancer Lett, 2015, 357(1):43-46.
[2] Terentiev AA, Moldogazieva NT.Alpha-fetoprotein: a renaissance[J]. Tumour Biol, 2013, 34(4):2075-2091.
[3] Kajiyama Y, Tian J, Locker J.Regulation of alpha-fetoprotein expression by Nkx2.8[J]. Mol Cell Biol, 2002, 22(17):6122-6130.
[4] Peterson ML, Ma C, Spear BT.Zhx2 and Zbtb20: novel regulators of postnatal alpha-fetoprotein repression and their potential role in gene reactivation during liver cancer[J]. Semin Cancer Biol, 2011, 21(1):21-27.
[5] Nakao K, Ichikawa T.Recent topics on alpha-fetoprotein[J]. Hepatol Res, 2013, 43(8):820-825.
[6] Forner A, Llovet JM, Bruix J.Hepatocellular carcinoma[J]. Lancet, 2012, 379(9822):1245-1255.
[7] Song PP, Xia JF, Inagaki Y, et al.Controversies regarding and perspectives on clinical utility of biomarkers in hepatocellular carcinoma[J]. World J Gastroenterol, 2016, 22(1):262-274.
[8] Tang H, Tang XY, Liu M, et al.Targeting alpha-fetoprotein represses the proliferation of hepatoma cells via regulation of the cell cycle[J]. Clin Chim Acta, 2008, 394(1):81-88.
[9] Parpart S, Roessler S, Dong F, et al.Modulation of miR-29 expression by alpha-fetoprotein is linked to the hepatocellular carcinoma epigenome[J]. Hepatology, 2014, 60(3):872-883.
[10] Chen L, Zhang W, Liang HF, et al.Activin A induces growth arrest through a SMAD-dependent pathway in hepatic progenitor cells[J]. Cell Commun Signal, 2014, 12:18.
[11] Zhao BB, Li HM, Gao X, et al.The herbal compound "diwu yanggan" modulates liver regeneration by affecting the hepatic stem cell microenvironment in 2-acetylaminofluorene/partial hepatectomy rats[J]. Evid Based Complement Alternat Med, 2015, 2015:468303.
[12] Sauzay C, Petit A, Bourgeois AM, et al.Alpha-foetoprotein (AFP): A multi-purpose marker in hepatocellular carcinoma[J]. Clin Chim Acta, 2016, 463:39-44.
[13] Wang B, Zhao L, Fish M, et al.Self-renewing diploid Axin2(+) cells fuel homeostatic renewal of the liver[J]. Nature, 2015, 524(7564):180-185.
[14] Tarlow BD, Finegold MJ, Grompe M.Clonal tracing of Sox9+ liver progenitors in mouse oval cell injury[J]. Hepatology, 2014, 60(1):278-289.
[15] Malato Y, Naqvi S, Schurmann N, et al.Fate tracing of mature hepatocytes in mouse liver homeostasis and regeneration[J]. J Clin Invest, 2011, 121(12):4850-4860.
[16] Shin S, Upadhyay N, Greenbaum LE, et al.Ablation of Foxl1-Cre-labeled hepatic progenitor cells and their descendants impairs recovery of mice from liver injury[J]. Gastroenterology, 2015, 148(1):192-202.