Mechanism of Protective Effect of Raddeanin A on Hepatic Ischemia-reperfusion in Rats

doi:10.12300/j.issn.1674-5817.2020.077

Abstract

Abstract: Objective To explore the protective mechanism of raddeanin A (RA) on hepatic ischemia-reperfusion injury in rats. Methods Thirty rats were randomly assigned into three groups including sham, model and RA groups (n=10). The rats in the RA group were injected with RA (10 mg/kg) in the tail vein 24 h and 1 h before model construction. The rats in the sham and model groups were injected with normal saline of equal volume. After the rats were anesthetized, the blood vessels entering the liver were blocked for 60 min, causing 70% of the liver to be ischemia. The contents of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) in serum were detected 6 h after liver blood flow was restored, the superoxide dismutase (SOD), malondialdehyde (MDA), interleukin-1? (IL-1?), interleukin-6 (IL-6) and tumor necrosis factor-〈 (TNF-〈) levels in liver were detected by ELISA. Liver specimens were stained with hematoxylin-eosin (HE) and TUNEL for observation of pathologic and apoptotic changes. Bcl2-associated X protein (Bax), Bcl-2 and Caspase-3 protein levels in liver were detected by Western blotting. Results The levels of ALT, AST and LDH in the RA groups were lower than those in the model group (P<0.01). The levels of MDA, IL-1b, IL-6, TNF-a, Bax and Caspase-3 in the RA groups were lower than those in the model group (P<0.05), the levels of SOD and Bcl-2 were higher (P<0.05). The structure of hepatic lobules was complete in the RA group. The apoptotic cells and the apoptosis rate in the RA group were lower than those in the model group (P<0.01). Conclusion Preoperative injection of RA can protect the liver by improving the liver function of rats after ischemia-reperfusion, reducing the release of oxidative and inflammatory factors, and reducing the apoptosis of hepatocytes via down-regulating the expressions of Bax and Caspase-3 proteins.

Key words: Raddeanin A, Hepatic ischemia-reperfusion, Apoptosis, Oxidative stress, in vivo activity, Active ingredients of Chinese medicine, Rats

LI Hongbo, HUANG Rui, DAI Jiang, ZHONG Zhendong. Mechanism of Protective Effect of Raddeanin A on Hepatic Ischemia-reperfusion in Rats[J]. Laboratory Animal and Comparative Medicine, 2021, 41(1): 55-60.

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References

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