Pathological Mechanism of Conjunctival Injury in Diabetic Model Rabbits

doi:10.3969/j.issn.1674-5817.2020.02.008

Abstract

Abstract: Objective To analyze matrix metalloproteinases (MMPs) and tissue inhibitor of matrix metalloproteinases (TMPs) in the conjunctival tissue cells of diabetic rabbits, and to analyze the relationship between MMPs and TMPs. Methods Twenty Japanese white rabbits were randomly divided into 2 groups, the rabbits in control group were fed with the normal experimental rabbit diet, while the diabetic experiment group was feed with high fat and high sugar diet for 8 weeks, and then injected with streptozourease by auricular vein for establishing diabetic model rabbits. Two weeks after the model was made, two groups of rabbits were anesthetized with general anesthesia, and one piece of 2 mm×3 mm bulbar conjunctiva tissue of each eye was sampled respectively. The right conjunctiva was used for pathological observation, and left eye conjunctiva was used for detecting the MMP-1, MMP-3, MMP-9 and TMP-1, TMP-3. Results The expressions of MMP-1, MMP-3, MMP-9 in the experiment group were high compared with those of the control group (P<0.05), and the expression of TMP-1 in the experimental group was slightly higher compared with that of the control group (P<0.05), but TMP-3 was significantly lower (P>0.05). In the diabetic experimental group rabbits, the conjunctival epithelium was arranged in order, subepithelial tissue was obviously reduced, conjunctival tissue was loose, atrophic fascia tissue was observed, the cells of the lamina propria of conjunctiva were loose, and the inflammatory cells between the cells were increased. Discussion Break down of the balance between MMPs and TMPs expression may produce elastic fibers in bulbar conjunctiva, and pathological changes of hypoconjunctiva tissue, the fascia atrophy changed the microenvironment of diabetic rabbit.

Key words: Matrix metalloproteinase, Tissue inhibitor of matrix metalloproteinase, Model of diabetic, Rabbit, Pathological examination of conjunctiva

CLC Number:

Q95-33

GE Jian, ZHU Caihong, WU Yanlin, WU Yongjie. Pathological Mechanism of Conjunctival Injury in Diabetic Model Rabbits[J]. Laboratory Animal and Comparative Medicine, 2020, 40(2): 136-140.

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