Abstract: Objective To express the glycoproteins of simian B virus, use them to detect the BV antibodies in serum,and search for the better antigens or their combination which have best sensitivity and specificity. Methods Express B virus glycoprotein C，D and G and check their antigenicity by Western Blot after purification and renaturation.The ELISA was established and evaluated. Results The three antigens react well with simian serum which BV antibody is positive. The sensitivity of the antigens is BVgC-ELISA 84.6%, BVgD-ELISA 88.5%，BVgC+gD-ELISA 96.2%, specificity is BVgC-ELISA 93%, BVgD-ELISA 85.7%, BVgC+gD-ELISA 64.3% according to the results of BV-ELISA. Conclusion The three antigens react well with simian serum and can be used as surrogate antigens of BV.

Key words: B vims, ELISA, Sensitivity, Specificity, Prokaryotic expression