Abstract: To study the morphological and quantitative changes of mast cell （MC） in scalding wound healing，the full-thickness scalding model were established in rats.The HE and Toluidine blue stain were used to detect the changes of MC in morphology and quantity, granulation tissue, and collagen fibers.The results showed that MC was degranulated in different degree after scalding. The quantity of MC decreased 24 hours later and reached the lowest level on the 3rd day. Then the quantity of MC increased gradually and reached the highest level on the 28th day. After that the quantity of MC decreased again.A little granulation tissue grew in the bottom and edge of the wound at 3 days after scalding. On the 14th day,the collagen fibers were found in the area of granulation tissue formed in the earlier stage.These findings suggest that there are three stages in the quantitative change of MC after scalding in rats，and the development of granulation tissue and collagen fibers is correlated with the changes of MC in the wound healing.

Key words: Mast cell, Scalding, Wound healing, Granulation tissue, Collagen fiber