Abstract: Objective To study the role of potential functional genes in the growth of mouse nails, and to establish and optimize mouse nail regeneration model of nail stem cells based on foreign research. Methods The 8-week-old C57BL/6 mice were selected as the experimental object, and precise position amputation surgery was performed after anesthesia under the microscope. The exploration and practice of a series of research methods such as animal modeling, real-time observation and recording, sampling measurement and section staining were carried out, and the research procedures were optimized as appropriate. Results A systematic study method for mouse nail regeneration animal model was developed. Conclusion This model can evaluate the growth of mouse nails at the animal level and tissue level, and it can be an effective means to study nail stem cells and related functional genes.

Key words: Nail regeneration, Length measurement, HE staining