The Immunity Effect Evluation on Tree Shrew by Hepatitis A Virus Attenuated Live Vaccine, Hepatitis B Vaccine Independently or Combined Adjuvant Heparan Sulfate

doi:10.3969/j.issn.1674-5817.2017.02.006

Abstract

Abstract: Objective To investigate the humoral immune response of tree shrew after the immunization of Hepatitis A attenuated live vaccine (HepA-l)、Hepatitis B vaccine (Hbv) independently and combined with adjuvant heparan sulfate (HS). Methods The tree shrews were randomly divided into 10 groups by the type of vaccine, adjuvant and schedules. The specific IgG levels in sera of tree shrew were determined by ELISA at 4, 8, 12, 16 and 20 weeks after the last immunization respectively. Results Except the blank control group, the anti - hepatitis A virus (HAV) and anti - hepatitis B virus (HBV) antibody was detected in all the experimental group at 4 weeks after last immunization, the level of immune antibody were gradually increased with time and reached the peak at the 12 weeks, then decreased gradually. At the same time, the humoral immunity effect of HS adjuvant group were better than that of vaccine group. There is a significant difference on immunity among tree shrews with different antigens and adjuvant by different immunization schedules. Conclusion The immune effect of vaccine immunogenicity and immune enhancing effect on tree shrew confirms the feasibility of the tree shrew as an animal model.

Key words: Tree shrew, Hepatitis A virus attenuated live vaccine (HepA-l), Hepatitis B vaccine (Hbv), Heparan sulfate (HS), Humoral immunity

CLC Number:

Q95-33

WANG Dong-bao, HU Yun-zhang, HU Ning-zhu, HAN Yuan-yuan, KUANG De-xuan, LI Yan-han. The Immunity Effect Evluation on Tree Shrew by Hepatitis A Virus Attenuated Live Vaccine, Hepatitis B Vaccine Independently or Combined Adjuvant Heparan Sulfate[J]. Laboratory Animal and Comparative Medicine, 2017, 37(2): 113-117.

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