Effect of β-carboline derivative on Subcutaneous Transplant Hepatocellular Carcinoma Growth in BALB/c Nude Mice

doi:10.3969/j.issn.1674-5817.2013.04.009

Abstract

Abstract: Objective To investigate the effect of â-carboline derivative HMD on subcutaneous transplant hepatocellular carcinoma in BALB/c nude mice. Methods A tumor model of human HCC was created by subcutaneous inoculation of 2×10⁷ (cells/ml,0.2ml) HepG2 cells into BALB/c male nude mice. The mice were randomized to low-(0.22 mg/kg), medium-(0.44 mg/kg), and high-dose HMD(0.87 mg/kg)group, saline group and 5-Fu group. The mice in each group received iv injection of the drug for 15 days. The effects of HMD on the growth of human hepatocellular carcinoma, the weight and pathological changes of nude miceŁ§S main organs were observed. Results Compared with saline group, the mice in HMD groups and 5-Fu group showed significant inhibition in the growth of the transplanted tumor (P<0.01).The inhibitory rate of HMD on the mouse weight. Compared to the saline group, the inhibitory effects of HMD(0.44, 0.87 mg/kg) were of statistic significance (P<0.01). Contrast to the negative control, there were no significant difference to the weight of kidney and kidney index in HMD groups and 5-Fu group, but there were significant differeces in the weight of liver and liver index (P<0.05). Conclusion HMD exhibit an inhibitory effect on the growth of transplanted HepG2 tumor, and the mice don’t appear toxicity symptom.

Key words: β- carboline derivatives(HMD), BALB/c nude mice, Subcutaneous transplanted liver tumor, Acute toxicity

CLC Number:

Q95-33

ZHANG Yan, FAN Wen-xi, XU Yi-mei, SHI Sheng, GUI You-jun, YAN Shun-sheng. Effect of β-carboline derivative on Subcutaneous Transplant Hepatocellular Carcinoma Growth in BALB/c Nude Mice[J]. Laboratory Animal and Comparative Medicine, 2013, 33(4): 290-295.

References

[1] 曹日晖. 去氢骆驼蓬碱结构优化与抗肿瘤构效关系研究[J/OL](2007-11-10) [2012-7-8]. http://ww.doc88.com/p-31079861158.html.
[2] 贾鹏飞. 新型β-咔啉类生物碱dichotomine和天然产物1,7-二芳基庚烷类化合物的全合成研究 [D]. 兰州: 兰州大学,2007, 4-9.
[3] 费洪新, 孙丽慧, 张涛, 等. 人肝癌HepG2细胞培养的体会[J]. 卫生职业教育, 2007, 25(8):138-139.
[4] 叶翩, 张淑玲, 揭盛华, 等. 人肝癌裸鼠移植模型的研究进展[J]. 世界华人消化杂志, 2006, 14(36):3500-3503.
[5] 田庆愕, 李玛琳, 孙汉董. 旱生香茶菜总二萜对荷人肝癌裸鼠皮下移植瘤生长的影响及其毒性研究[J]. 药品评价,2005, 2(4):268-272.
[6] 吴永忠, 任庆兰, 李少林. 脂质体c-erbB-2反义寡核苷酸对人卵巢癌裸鼠皮下移植瘤的治疗作用[J]. 实用癌症杂志, 2003, 18:6-8.
[7] Liu XY, Hu YX, Hu QM, et al.To compare the model of hepatic fibrosis between rat by CCl4 and pig by serum[J]. World Chinese Journal of Digestology, 2002, 10(8):975-977.
[8] 刘建文, 季光, 魏东芝. 药理实验方法学[M]. 北京: 化学工业出版社, 2003:21.
[9] Xu S Y, Bian R L, Chen X.Methodology in Pharmacological Experiment[M]. The third edition, Beijing:People’s Medical Publishing House, 2002,202-204.
[10] 李幼平. 医学实验技术的原理与选择[M]. 北京: 人民卫生出版社, 2008.28.
[11] Dykes DJ, Abbott BJ, Mago JG, et al. Development of evaluation of human tumor xenography modes for in vivo evalution of new antitumor drugs[C]. Feibig H H,Bergerp,eds.Immunodeficant mice in encology, Basel Kanger,1992, 2:l-22.
[12] 卢永刚, 谭晶, 张洁, 等. 茉莉酸甲酯对人肝癌细胞HepG-2裸鼠皮下移植瘤生长抑制作用的研究[J]. 昆明医学院学报, 2007, (6):24-28.
[13] 张东兴, 颜登国, 赵丙波. 人大肠癌裸鼠皮下移植瘤模型的建立[J]. 贵阳医学院学报, 2010, 35(3):247-250.
[14] 国家食品药品监督管理局药品评审中心. 抗肿瘤药物药效学指导原则[S/OL]. (2012-3-1). http://www.doc88.com/p-443541074498.html.