Abstract: Objective To observe the oxygen free radical change in sub-healthy rat induced by restraint stress and to explore the mechanism of Traditional Chinese Medicine alters the stress.Methods Fourty Male SD rats, weighted 320～360g, were divided randomly into 4 groups, which including normal control group, model control group, anti-aging tablets group and Liuweidihuang pills group, To simulate human physiological stress, the sub-healthy rat model was induced by restraint，meanwhile, the rats in anti-aging tablets group were administrated by anti-aging tablets and Liuweidihuang pills group were treated by Liuweidihuang pills separately. Fourteen and 21 days after experiment，5 rats were selected randomly in each group to collect vein blood in order to test the activities of SOD,GSH-Px and the content MDA、LD. Results The activity of SOD and GSH-Px in blood serum in model rats lowered obviously (P<0.05, P<0.01) while the MDA、LD ascended significantly (P<0.05). The activity of SOD and GSH-Px in serum increased and the content of serum MDA、LD decreased significantly in anti-aging tablets group (P<0.05, P<0.01). Conclusion The restraint stress for 3 hours everyday which could initiate the sub-healthy syndrome, and the antioxidation of model rat was decreased.The treatment of anti-aging tablets and Liuweidihuang pills could improve the sub-healthy state and the mechanism may be related to the effects on antioxidation.

Key words: Restraint stress, Sub-health rats, Anti-aging tablet, Liuweidihuang pills;Free radicals metabolism