Abstract: Objective To improve embryo collecting method for rat microinjection. Methods PMSG-HCG(ip) were injected into 5 weeks SD rats. Embryos were collected by tearing intumescences of oviduct or clysising fimbriae tubae. The amount of embryos, rates of fertilization, and survival rate of embryos after injection were analyzed to compare the two methods. Results The amount of embryos, rates of fertilization, and survival rates of embryos after injection of the embryos collected by clysising fimbriae tubae were higher than those collected by tearing intumescence of oviduct. There are significant differences between the two methods. Conclusion Clysising fimbriae tubae is a better embryo collecting method for rat microinjection.

Key words: Rat, Microinjection, Embryo collecting