Abstract: Objective To Clone the gene of TNNT2 and analyze its expression. Methods Total RNA was extracted from the heart of adult SD rat and first strand cDNA was obtained by reverse transcription. Primers was designed according to the offered sequence in Genebank. TNNT2 cDNA was cloned by PCR and confirmed by sequencing，RT-PCR was applied to examine the expression pattern of TNNT2 in rat tissues. Results The TNNT2 cDNA sequence was cloned and submitted it to Genebank (Genebank acc.No.EU295527), the TNNT2 was expressed in many tissues and had the highest expression level in heart. Conclusions Rat TNNT2 cDNA sequence was first cloned(Genebank Acc.No.EU295527), TNNT2 was expressed in many tissue and had the highest expression level in heart.

Key words: Rat, TNNT2, Hypertrophic, Gardiomyopathy