Effect of Kappa-selenocarrageenan on Humoral Immune Response Induced by Hepatitis a Virus Attenuated Live Vaccine in Mice

doi:10.3969/j.issn.1674-5817.2018.02.006

Abstract

Abstract: Objective To investigate the effect of kappa-selenocarrageenan (KSC) on humoral immune response induced by hepatitis A virus attenuated live vaccine (HepA-1) in mice.Methods Seven experiment groups were set up,with 7 ICR mouse per group randomly,negative control group (saline),pure antigen group (18EU HepA-1),Aluminium adjuvant group [Al(OH)₃ 0.3 mg+18 EU HepA-1],KSC experimental groups (KSC 1 mg+18 EU HepA-1,KSC 0.5 mg+18 EU HepA-1,and KSC 0.25 mg +18 EU HepA-1,KSC 0.1 mg+18 EU HepA-1),in which mice were injected subcutaneously with 300 µL,immunizing one time.The anti-HAV specific IgG antibody levels were tested by indirect ELISA method in the serum of mice after the first 4th,8th,12th,16th week of injection.The healthy status of mice were observed during the study.The kidney,spleen,liver,lung and heart of mice immunized with KSC+HepA-l at the optimal dosage and those in negative control group were taken out at 16th week after immunization,and prepared into sections for pathological observation.Results HAV-specific IgG antibody of all mice were detected at four setting time point except negative control group.The antibody level of all groups increased first and then decreased.The peak of Aluminium adjuvant group at the 12th week and others at the 8th week.The level of antibody in KSC 0.5 mg+18EU HepA-1 group at 8th,12th week was higher than that in simple antigen group (t=3.103,t=2.573,P<0.05).The level of antibody in KSC 0.25 mg +18EU HepA-1 group at week 12 was higher than that in pure antigen group(t=2.602,P<0.05).The optimal dosage of mice in this test was 0.5 mg.No abnormality was observed in the physiological indexes of mice in various groups,while there was no pathological changes in the kidney,spleen,liver,lung and heart of mice immunized with HepA-l +0.5 mg KSC.Conclusion KSC can be used as an adjuvant to enhance humoral immune response induced by HepA-1 in mice.

Key words: Kappa-selenocarrageenan(KSC), Adjuvant, Hepatitis A virus attenuated live vaccine (HepA-1), Humoral immune

CLC Number:

Q95-33

JIA Sen-quan, JIN Xiao, LI Xiao-hong, WANG Hai-xuan, HU Ning-zhu, HU Yun-zhang. Effect of Kappa-selenocarrageenan on Humoral Immune Response Induced by Hepatitis a Virus Attenuated Live Vaccine in Mice[J]. Laboratory Animal and Comparative Medicine, 2018, 38(2): 111-116.

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