Effects of Indoleamine-2,3-dioxygenase Inhibitor Combined with Aluminum Adjuvant on Humoral Immune Response Induced by Hepatitis A Virus Attenuated Live Vaccine in Mice

doi:10.3969/j.issn.1674-5817.2017.02.005

Abstract

Abstract: Objective To investigate the compound effect of indoleamine-2,3-dioxygenase (IDO)inhibitor INCB024360 analogue combined with aluminum adjuvant on humoral immune response induced by hepatitis A virus (HAV) attenuated live vaccine(HepA-l) in mice. Methods The ICR mice were injected respectively with HepA-l (18EU) and Al(OH)₃ (300 μg) and three different doses (150 μg, 100 μg, 50 μg) of INCB024360 analogue as experimental group, 200 μL for each and one immunization. At the same time, set up the blank group, pure vaccine group, aluminum adjuvant control group and single INCB024360 analogue (100 μg) group. The sera from tail vein blood of mice were collected at the end of 4, 8, 12 and 16 weeks after immunization and the specific IgG antibody against HAV were detected by indirect ELISA method. Results In addition to the blank group, mice in various group generated anti-HAV IgG at 4, 8, 12, 16 weeks after the immunization. The antibody levels increased as time went on and reached peak at 12 weeks, then decreased gradually. The antibody level of compound adjuvant 2 group is the highest. At 8, 12 weeks, the antibody level was significantly higher than pure vaccine group, aluminum adjuvant control group and single INCB024360 analogue group (P<0.05). At 4, 8, 12, 16 weeks, the difference between the single adjuvant group and the simple vaccine group was not statistically significant. Conclusions The compound adjuvant of IDO inhibitor INCB024360 analogue combined with aluminum adjuvant can obviously enhance the HepA-l induced humoral immune response in mice, and the immunity enhancement effect is better than aluminum adjuvant control group. But the single INCB024360 analogue does not have significant adjuvant effect.

Key words: Indoleamine-2,3-dioxygenase (IDO), INCB024360 analogue, Vaccine adjuvant, Hepatitis A virus attenuated live vaccine (HepA-l), Humoral immunity, Adjuvant effect

CLC Number:

Q95-33

HE Hui, LI Yan-han, LI Jian-fang, MA Jing, HU Yun-zhang, HU Ning-zhu. Effects of Indoleamine-2,3-dioxygenase Inhibitor Combined with Aluminum Adjuvant on Humoral Immune Response Induced by Hepatitis A Virus Attenuated Live Vaccine in Mice[J]. Laboratory Animal and Comparative Medicine, 2017, 37(2): 108-112.

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